Gonadotropin inhibitory hormone downregulates steroid hormone secretion and genes expressions in duck granulosa cells

Chen, Shijian; Liu, Wenjun; Yang, Chen; Li, Xiujin; Shen, Xu; Jiang, Danli; Huang, Yunmao; Tian, Yunbo

doi:10.1590/1984-3143-ar2021-0036

Cited by 7 publications

(5 citation statements)

References 38 publications

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“…To further validate the important role of GnIH at the gonadal level in the regulation of gonadotropin receptor expression, we conducted in vitro cellular experiments. Isolating quail granulosa cells in vitro and adding GnIH treatment, we found a significant decrease in key factors of the steroid synthesis pathway, and previous studies showed that GnIH treatment dose-dependently inhibited the levels of 3β-HSD, CYP19A1 and StAR in duck granulosa cells ( Chen et al, 2021 ), similar results were found in mammalian studies, where GnIH treatment dose-dependently downregulated the expression levels of 3β-HSD and StAR to inhibit steroid hormone synthesis ( Singh et al., 2011b ). At the same time, GnIH treatment significantly down-regulated LHR expression levels, indicating a direct inhibitory effect of GnIH on follicle development and ovulation, further verifying that the effect of photoperiod alteration on follicle development and ovulation in vivo was caused by changes in GnIH expression levels.…”

Section: Discussionsupporting

confidence: 86%

“…After centrifuging the filtrate at 1,000 rpm for 5 min at 4°C, it was resuspended in M199 medium containing 10% FBS and 100 U/mL of penicillin or streptomycin. Next, the cells were plated at a density of 5 × 10 5 /mL cells in 6-well plates (Corning Costar) and cultured with 5% CO 2 at 39°C ( Yu et al, 2017 ; Wu et al, 2019 ; Chen et al, 2021 ). When cells had reached confluence, the medium was replaced with M199 containing different concentrations of GnIH (0, 1, 10, and 100 ng/mL, GnIH: SIKPSAYLPLRF, Phoenix pharmaceuticals, inc., Beijing, China) for 24 h, each treatment was repeated three times.…”

Section: Methodsmentioning

confidence: 99%

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Expression of GnIH and its effects on follicle development and steroidogenesis in quail ovaries under different photoperiods

et al. 2022

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Section: Discussionsupporting

confidence: 86%

Section: Methodsmentioning

confidence: 99%

Expression of GnIH and its effects on follicle development and steroidogenesis in quail ovaries under different photoperiods

et al. 2022

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“…Thus, we speculated that RFRP-3 inhibited follicular growth by increasing the GnIH concentration in vivo and blocked the crucial pathway of steroidogenesis mostly via reducing the mRNA abundances of LHR , 3β-HSD , and StAR . Similarly, granulosa cells of quail and duck showed a considerable dose-dependent reduction of the expression levels of the core factors in the steroidogenic pathway [ 30 , 47 ]. Consistent with our findings, treatment with GnIH dose-dependently decreased the mRNA abundances of StAR and 3β-HSD and repressed the synthesis of the steroid hormone in mice [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

RFRP-3 Influences Apoptosis and Steroidogenesis of Yak Cumulus Cells and Compromises Oocyte Meiotic Maturation and Subsequent Developmental Competence

Xiong

Pan

et al. 2023

IJMS

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RF amide-related peptide 3 (RFRP-3), a mammalian ortholog of gonadotropin-inhibitory hormone (GnIH), is identified to be a novel inhibitory endogenous neurohormonal peptide that regulates mammalian reproduction by binding with specific G protein-coupled receptors (GPRs) in various species. Herein, our objectives were to explore the biological functions of exogenous RFRP-3 on the apoptosis and steroidogenesis of yak cumulus cells (CCs) and the developmental potential of yak oocytes. The spatiotemporal expression pattern and localization of GnIH/RFRP-3 and its receptor GPR147 were determined in follicles and CCs. The effects of RFRP-3 on the proliferation and apoptosis of yak CCs were initially estimated by EdU assay and TUNEL staining. We confirmed that high-dose (10−6 mol/L) RFRP-3 suppressed viability and increased the apoptotic rates, implying that RFRP-3 could repress proliferation and induce apoptosis. Subsequently, the concentrations of E2 and P4 were significantly lower with 10−6 mol/L RFRP-3 treatment than that of the control counterparts, which indicated that the steroidogenesis of CCs was impaired after RFRP-3 treatment. Compared with the control group, 10−6 mol/L RFRP-3 treatment decreased the maturation of yak oocytes efficiently and subsequent developmental potential. We sought to explore the potential mechanism of RFRP-3-induced apoptosis and steroidogenesis, so we observed the levels of apoptotic regulatory factors and hormone synthesis-related factors in yak CCs after RFRP-3 treatment. Our results indicated that RFRP-3 dose-dependently elevated the expression of apoptosis markers (Caspase and Bax), whereas the expression levels of steroidogenesis-related factors (LHR, StAR, 3β-HSD) were downregulated in a dose-dependent manner. However, all these effects were moderated by cotreatment with inhibitory RF9 of GPR147. These results demonstrated that RFRP-3 adjusted the expression of apoptotic and steroidogenic regulatory factors to induce apoptosis of CCs, probably through binding with its receptor GPR147, as well as compromised oocyte maturation and developmental potential. This research revealed the expression profiles of GnIH/RFRP-3 and GPR147 in yak CCs and supported a conserved inhibitory action on oocyte developmental competence.

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“…26 Both estrone and progesterone were significantly downregulated in duck granulosa cells treated with gonadotropin inhibitory hormone compared to the control. 27 Additionally, miRNAs also have a regulatory effect on steroidogenesis in granulosa cells, which is achieved by direct binding to their target genes. miR-202−5p restrained lipid deposition and steroidogenesis in goose granulosa cells through targeting the acyl-CoA synthetase long-chain family member 3 (ACSL3) gene.…”

Section: Discussionmentioning

confidence: 99%

“…Heat stress inhibited estradiol synthesis in duck granulosa cells by downregulating the expression of CYP11A1 and CYP19A1 . Both estrone and progesterone were significantly downregulated in duck granulosa cells treated with gonadotropin inhibitory hormone compared to the control . Additionally, miRNAs also have a regulatory effect on steroidogenesis in granulosa cells, which is achieved by direct binding to their target genes.…”

Section: Discussionmentioning

confidence: 99%

Lipid Deposition and Progesterone Synthesis Are Increased by miR-181b-5p through RAP1B/ERK1/2 Pathway in Chicken Granulosa Cells

Lin,

Gong,

Sun

et al. 2023

J. Agric. Food Chem.

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Steroid hormones secreted by granulosa cells are essential for maintaining normal development of chicken follicles. Our previous sequencing data indicated that miR-181b-5p and RAS-related protein 1B (RAP1B) appeared to function in chicken granulosa cells, which was further explored in this study. The results suggested that miR-181b-5p facilitated the aggregation of lipid droplets and the synthesis of progesterone. In contrast, RAP1B astricted lipid deposition and progesterone secretion. Cotransfection of the RAP1B overexpression vector with miR-181b-5p mimic eliminated the promoting effect of miR-181b-5p. Dual-luciferase reporter assay confirmed that miR-181b-5p bound directly to the 3′ untranslated region (3′ UTR) of RAP1B. We also found that miR-181b-5p and RAP1B reduced and enhanced the phosphorylation levels of extracellular signal-regulated kinases 1 and 2 (ERK1/ 2), respectively. The application of ERK1/2 activators and inhibitors demonstrated that ERK1/2 is a negative regulator of lipid deposition and progesterone synthesis. In conclusion, we revealed that miR-181b-5p accelerated lipid deposition and progesterone synthesis through the RAP1B/ERK1/2 pathway in chicken granulosa cells. miR-181b-5p and RAP1B may serve as new biomarkers in breeding to improve chicken reproductive performance and prevent ovary-related diseases.

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Gonadotropin inhibitory hormone downregulates steroid hormone secretion and genes expressions in duck granulosa cells

Abstract: inhibitory hormone downregulates steroid hormone secretion and genes expressions in duck granulosa cells. Anim Reprod. 2021;18(2):e20210036.

Cited by 7 publications

References 38 publications

Expression of GnIH and its effects on follicle development and steroidogenesis in quail ovaries under different photoperiods

Expression of GnIH and its effects on follicle development and steroidogenesis in quail ovaries under different photoperiods

RFRP-3 Influences Apoptosis and Steroidogenesis of Yak Cumulus Cells and Compromises Oocyte Meiotic Maturation and Subsequent Developmental Competence

Lipid Deposition and Progesterone Synthesis Are Increased by miR-181b-5p through RAP1B/ERK1/2 Pathway in Chicken Granulosa Cells

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