Polyphenol analysis in human milk by a rapid, cost-effective, and accurate micromethod: translational development
Mariela Valentina Cortez,
Ana Veronica Scotta,
Agustín Ramiro Miranda
et al.
Abstract:Objective: To develop a rapid method for analysing polyphenols, which are potentially active antioxidants against neonatal oxidative stress, from small human milk (HM) volumes. Methods: Acid and alkaline extractions were compared using two dyes: Folin-Ciocalteu and Fast Blue BB. Linearity, sensitivity, recovery percentage, polyphenol content, precision, and stability were assessed in 14 HM samples and compared using the Kruskal-Wallis H test (p<0.05). The best technique was applied to 284 HM samples to det… Show more
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