Control of contaminants in the in vitro establishment of Guadua latifolia

Abstract

The Amazonian bamboo forests are located in an important region of high biodiversity in Brazil, Peru and Bolivia, forming the largest native bamboo forest reserve in the world. However, the bamboos from these forests have characteristics that hinder their propagation. This study aimed to evaluate the biocide action of a plant preservative mixture for controling contaminants, during the in vitro establishment of Guadua latifolia (Bonpl.) Kunth, a species native to the region. Nodal segments were cultured in a semi-solid medium containing Plant Preservative Mixture (PPMTM), at the concentrations of 0; 1; 2; and 3 mL L-1, and supplemented with 2 mg L-1 of 6-benzylaminopurine. The analyzed variables were number of shoots, percentage of bacterial and fungal contamination, and shoot survival. The treatments with the synthetic biocide were efficient in controlling the in vitro contamination caused by bacteria and fungi (Fusarium sp.), also presenting the highest survival rate of regenerated shoots. For the in vitro establishment of this native bamboo species, the use of 2 mL L-1 of PPMTM is recommended.

Indução da calogênese em segmentos foliares de seringueira (Hevea spp.) na Amazônia Sul-Ocidental

“…Vale ressaltar, que muitos contaminantes são observados somente após algumas semanas de cultivo, o que indica a possibilidade de microrganismos endofíticos existentes nos tecidos (Palu, 2011). Afim de contornar essa barreira, estudos tem apontado o uso satisfatório do PPM ® na eliminação dos contaminantes em várias espécies na cultura de tecidos vegetais (Dutra et al, 2008;Leão et al, 2020). Compton e Koch (2001), ao investigar os efeitos de diferentes concentrações de PPM ® na organogênese in vitro de melão (Cucumis melo L.), petúnia (Petunia Juss) e tabaco (Nicotiniana L.), constataram que resposta morfogenética referente ao uso de diferentes concentrações do biocida depende de cada espécie, podendo ou não afetar a regeneração in vitro.…”

In vitro multiplication of woody bamboo in the Southwestern Amazon, Acre State, Brazil

“…Following selection of the optimal sterilization treatment (Leão et al, 2020), the explants were inoculated in glass tubes containing 10 mL of MS liquid medium (Murashige & Skoog, 1962), supplemented with sucrose (30 g L -1 ), 2 mL L -1 of Plant Preservative Mixture -PPM ® and 6-benzylaminopurine (BA) concentrations of 0, 2, 4, 6 and 8 mg L -1 . The analyzed variables after 2 subcultures were number of shoots, shoot height, multiplication rate and the presence of callus and roots.…”

“…Further research is needed to determine the most appropriate propagation method and a seedling production system with high survival rates for G. latifolia in this region (Leão et al, 2020).…”

“…The production of bamboo seedlings on an industrial scale using micropropagation is recent. However, microbial contamination is still the main problem when establishing in vitro cultures (Nadha et al, 2012;Leão et al, 2020;Silva et al, 2021).…”

In vitro establishment from mature nodal segments of Persian Lime (Citrus latifolia Tan.)

“…PPM has been used to disinfect in vitro explants of species such as bamboo, strawberry, red ginger, etc. [11][12][13][14]. Likewise, Chlorothalonil is a broad-spectrum fungicide with a polychlorinated aromatic component that delays mycelial growth and inhibits spore germination.…”