PROPAGATION OF ANTIMICROBIAL RESISTANT Salmonella spp. IN BIVALVE MOLLUSKS FROM ESTUARY AREAS OF BAHIA, BRAZIL

Abstract

-In recent years, the emergence of resistant pathogens has complicated the treatment of bacterial infections in livestock production as well as in the medical field, due to the development of resistance mechanisms by microorganisms. The objective of this study was to delineate the antimicrobial resistance profile of Salmonella spp. strains isolated from bivalve mollusks (oysters and mussels) and from estuarine environment water of two regions of Bahia, Brazil. Twenty-seven strains, 12 isolated from bivalve mollusks and 15 from estuarine water, were tested. Eight antimicrobial agents (phenicol, beta-lactams, tetracyclines, quinolones and fluoroquinolones classes) were used for a susceptibility test, Minimum Inhibitory Concentration (MIC) and extended-spectrum beta-lactamases (ESBLs) production. Isolates showed high susceptibility to the classes of antimicrobial agents tested, with resistance only to nalidixic acid (27%), ampicillin (25%) and tetracycline (25%). Bacterial resistance was of chromosomal origin and the multidrug resistance index (MAR) among isolates of shellfish (mussels in natura) was 0.25. The MIC was found to be 100 µg/mL, 500 µg/mL and 350 µg/mL to nalidixic acid, ampicillin and tetracycline, respectively. None of the isolates presented ESBLs production. The presence of multidrug-resistant and high MIC Salmonella spp. is being conveyed in extraction areas of bivalve mollusks in the State of Bahia, Brazil.

Antimicrobial Susceptibility Testing for Salmonella Serovars Isolated from Food Samples: Five-Year Monitoring (2015–2019)

“…As expected, the occurrence of resistant Salmonella strains isolated from molluscan shellfish was significantly lower compared with the occurrence reported for poultry, pork, and bovine. However, the level of resistance reported in the present work was higher than that reported by [ 20 ]. Molluscan shellfish are filter-feeding animals, and the presence of resistant Salmonella serovars in them may indicate an alteration of water ecosystems by human action [ 21 ].…”

Qualidade microbiológica da água e dos mexilhões Perna perna (Linnaeus, 1758) cultivados em Piúma, Espírito Santo, Brasil

“…Observou-se que a quantidade de CT na água do cultivo oscilou de < 2 até > 1.600 NMP/100 mL, já o Ctt variou de < 2 até 375 NMP/100 mL, ambos os grupos com os maiores níveis no mês de janeiro, conforme apresentado na (Tabela 1). SILVEIRA et al, 2016).…”

Assessing the impacts of native freshwater mussels on nitrogen cycling microbial communities using metagenomics

“…Furthermore, our detected biomarkers for increased Phd-Doc toxin-antitoxin genes, are attributed with biochemical processes including antibiotic resistance [274]. One explanation for these findings may be related to mussels hosting antibiotic resistant symbionts [275,276], multidrug-resistant pathogens, [277], and enhanced horizontal gene transfer within their gut microbial community [278]. Previous research has also shown that mussels bioaccumulate various metals [279,280], including elements found in this biomarker study, copper and zinc [281].…”