Anatomia e histoquímica da lâmina foliar de espécies conhecidas por quebra-pedra (Euphorbia prostrata Aiton, Euphorbia hyssopifolia L., Phyllanthus amarus Schumach. &amp; Thonn e Phyllanthus tenellus Roxb.)

Conceição, Leda; Aoyama, Elisa Mitsuko

doi:10.1590/1983-084x/15_226

Cited by 1 publication

(2 citation statements)

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“…In samples of plants grown in a greenhouse, it was possible to identify 5-demethoxy-niranthin lignans (Rt: 16.403 min, M: 402 g/mol), phyllanthin (Rt: 16.908 min, M: 418 g/mol), 5-demethoxy-nirtetralin (Rt: 17.336 min, M: 400 g/mol), nirtetralin (Rt: 18.202, M: 430 g/mol), niranthin (Rt: 19.854 min, M: 432 g/mol), and filtetralin (Rt: 16,91, M: 416 g/mol), as well as other unidentified molecules. Niranthin and nirtetralin, similar to phyllanthin and hypophyllanthin, also have an antiviral effect [ 3 , 19 , 20 ].…”

Section: Discussionmentioning

confidence: 99%

“…It is a cosmopolitan plant with a wide distribution in the tropics and subtropics, as well as in the American continent, occurring mainly in Brazil and the Caribbean [ 2 ]. It is a medicinal plant popularly known as stonebreaker, as it has historically been used in the elimination of kidney stones, commonly used as an herbal medicine [ 3 ]. Its clinical effects include antihepatotoxic, antilytic, antihypertensive, anti-inflamatory, anti-human immunodeficiency virus (HIV), and anti-hepatitis B activities [ 4 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

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In Vitro Calli Production Resulted in Different Profiles of Plant-Derived Medicinal Compounds in Phyllanthus amarus

2020

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The efficient production of plant-derived medicinal compounds (PDMCs) from in vitro plants requires improvements in knowledge about control of plant or organ development and factors affecting the biosynthesis pathway of specific PDMCs under in vitro conditions, leading to a realistic large-scale tool for in vitro secondary metabolite production. Thus, this study aimed to develop an in vitro technique, through the induction and proliferation of calli, for production of plant fresh weight, and to compare the PDMC profile obtained from the plants versus in vitro calli of Phyllanthus amarus. It was successfully possible to obtain and proliferate two types of calli, one with a beige color and a friable appearance, obtained in the dark using Murashige and Skoog (MS) medium plus 2,4-dichlorophenoxyacetic acid (2,4-D), and a second type with a green color, rigid consistency, and nonfriable appearance obtained under light conditions and MS medium plus 6-benzyladenine (6-BA). In vitro micropropagated plants that gave rise to calli were also acclimatized in a greenhouse and cultivated until obtaining the mass for PDMC analysis and used as a control. While the micropropagated-derived plants concentrated the lignans niranthin, nirtetralin, and phyllanthin, the Phyllanthus amarus calli proliferated in vitro concentrated a completely different biochemical profile and synthesis of compounds, such as betulone, squalene, stigmasterol, and β-sitosterol, in addition to others not identified by GC-MS database. These results demonstrate the possibility of applying the calli in vitro from Phyllanthus amarus for production of important PDMCs unlike those obtained in cultures of differentiated tissues from field plants.

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Section: Discussionmentioning

confidence: 99%