Ação antiproliferativado flavonoide morina e do extrato da folha de oliveira (Oleaeuropaea L.) contra a linhagem de célula H460

Abstract

RESUMO:O presente estudo investigou a indução de morte celular por apoptose pelo flavonóide morina e pelo extrato da folha de oliveira (Oleaeuropaea L.) em linhagens de células de câncer de pulmão do tipo não pequenas (H460). O tratamento com morina e o extrato de oliveira em células H460 resultou na redução do crescimento tumoral e indução de morte celular avaliados pelos ensaios de MTT e lactato desidrogenase (LDH) e a morte celular por apoptose confirmada por microscopia de fluorescência e análise por citometria de fluxo. Os dados indicaram que o flavonóide morina e o extrato de oliveira diminuíram a viabilidade celular para taxas percentuais de 7,22± 1,54% e 62,37± 2,85% nas concentrações de 800µM e µg/mL, respectivamente. As maiores taxas percentuais de morte celular por apoptose foram100% para morina na concentração de 800µM e 70,49 ± 5,91% para o extrato de oliveira na concentração de 800 µg/ mL. Estes resultados foram associados com a alteração do potencial de membrana mitocondrial, cujos valores são de 54,91% para morina na concentração de 400µM e 42,2% para o extrato de oliveira na concentração de 800 µg/mL sugerindo envolvimento da via intrínseca da morte celular por apoptose. Portanto, morina e o extrato de oliveira afetaram a viabilidade celular da linhagem H460 induzindo morte celular por apoptose. Palavras-chave:Linhagens de células H460, morina, extrato de oliveira, atividade antitumoral. ABSTRACT:The antiproliferative action of morin flavonoid and olive leaf extract (Olea europaea L.) against the cell line H460. This study investigates possible apoptosis induction mechanism by the flavonoid morin and the olive leaf extract (Olea europaea L.) in non-small lung cancer cells (H460). The treatment with morin and olive leaves extract resulted in growth inhibition and induction of apoptosis in H460 cells lines measured by the MTT assay methods and confirmed by fluorescence microscopy, flow cytometry analysis. The data indicated that themurin and the extract of olive decreased the cell viability percentage rates of 7.22 ± 1,54% and 62.37 ± 2,85% in the concentrations of 800 µM and µg/mL, respectively. The highest percentage rates of cell death by apoptosis were 100% for themorin in a concentration of 800 µM and 70.49 ± 5.91% for the olive extract in a concentration of 800 µg/mL. These findings were associated with altered mitochondrial membrane potential, whose value is 54.91% for the murin concentration of 400 µM and 42.2% for the olive extract in a concentration of 800 mg / mL, suggesting involvement of the intrinsic pathway of cell death by apoptosis. Therefore, the morin and the olive extract affect the cell viability of H460 cell lines inducing cell death by apoptosis.

Comparative evaluation of plant extract effects on peritoneal, medullary and J774 cells. G8 macrophages

“…Nevertheless, natural products could cause only a transient and nonlethal effect, requiring evaluation by the LDH release assay. The identification of this enzyme in the medium is proportional to the number of cells killed by an in vitro necrosis process, where there is rupture of the cell membrane and LDH extravasation (Pereira et al, 2015).…”

Study of the Biological Activity and Phenolic Content of the Ethanol Extract of Phyllogonium viride Brid. (Phyllogoniaceae, Bryophyta)

“…The results obtained from the antioxidant capacity by the DPPH method has been presented in different ways by different authors and the lack of standardization of the results makes it difficult to compare the antioxidant capacity of the same sample, or different samples. [49] Although many of the existing antioxidant results are expressed as EC 50 , the experimental studies carried out by Oliveira [50] indicate a non-linear relationship between the antioxidant concentration and the DPPH radical scavenging activity, and therefore, the interpretation of the antioxidant results by EC 50 value may not be suitable.…”

“…The ethanolic extract was obtained by cold static maceration according to the methodology of Oliveira [50] Ethyl alcohol at 96°GL was used as extractor liquid. The plant material (100 g) was washed in abundant water from the municipal supply network and, later, in distilled water; drying took place at room temperature.…”

Use of microstructural characteristics of pollen and pistil to identify olive cultivars in Southern Brazil

“…The olive cultivation has spread throughout the world by clonal replicas of a diverse group of cultivars, whose main origin is the Mediterranean Sea Basin (Mora, Tapia, Ibacache, Nunes-Martins, & Alberto-Scapim, 2008). However, there are in the olive tree classification, more than 3000 synonyms and homonyms still to be reclassified (Özkaya, Ergülen, Ülger, & Özilbey, 2004;Rallo et al, 2005;Pereira, Oliveira, Kanashiro, & Costa, 2015;Adakalic & Lazovic, 2018), generating confusion and uncertainty about their correct identity (Özkaya et al, 2004;Alba, Montemurro, Sabetta, Pasqualone, & Blanco, 2009). This identity problem is caused because the characters used to classify them are usually influenced by local environmental fluctuations (Levin & Lavee, 2005;Mora et al, 2008;Besnard, Rubio de Casas, Christin, &Vargas, 2009;Matías, Toro, Montalván, & Molina, 2010;Lubna et al, 2019;Ali et al, 2021).…”