Micropropagation of Pluchea sagittalis (Lam.) Cabrera

Rossato, Liana Verônica; Canto-Dorow, Thais Scotti do; Nicoloso, Fernando Teixeira

doi:10.1590/1983-084x/13_106

Cited by 4 publications

(3 citation statements)

References 19 publications

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“…Vem sendo aplicada em diversos países, inclusive no Brasil, em inúmeras espécies de plantas. Esta técnica permite a produção de uma grande quantidade de plantas fisiologicamente uniformes e a sua rápida multiplicação, a fim de extrair substâncias de interesse (ROSSATO et al, 2015), evitando assim a exploração e degradação das populações selvagens e visando a sua conservação (COSTA et al, 2019).…”

Section: Resultsunclassified

Micropropagação de plantas medicinais no contexto brasileiro: revisão de literatura

Rojas¹,

Malosso²

2022

Revista Científica Multidisciplinar Núcleo do Conhecimento

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As plantas medicinais são utilizadas pelo homem desde os primórdios, contudo, a extração destas plantas tem sido em excesso e de forma irracional, colocando em risco a sua existência. Assim, torna-se importante buscar algumas alternativas que sejam capazes de auxiliar no aumento da produção e reprodução destas plantas. Este estudo busca responder à seguinte pergunta: o que é micropropagação de plantas medicinais no contexto brasileiro? Portanto, este estudo tem como objetivo, discutir sobre a micropropagação de plantas medicinais no contexto brasileiro. Para isso, conduziu-se uma revisão narrativa, considerando artigos e relatórios técnicos publicados entre 2014 a 2021, em português e inglês, em bases de dados brasileiras. Como resultado, observou-se que a micropropagação é uma técnica da Biotecnologia, para propagar plantas dentro de tubos de ensaios, em um curto período, livres de vírus e doenças, sendo cada vez mais empregadas para fins comerciais, agrícola, farmacêutico, cosmético e alimentar. Alguns laboratórios – “biofábricas”, estão se dedicando à essa técnica de multiplicação. Concluindo, a micropropagação de plantas medicinais permite a produção em larga escala, podendo ser usadas na indústria farmacêutica, gerando economia para o país, sem que ocorra a extinção das espécies vegetais na natureza.

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Section: Resultsunclassified

Micropropagação de plantas medicinais no contexto brasileiro: revisão de literatura

Rojas¹,

Malosso²

2022

Revista Científica Multidisciplinar Núcleo do Conhecimento

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“…Micropropagation of members of Asteraceae family has been reported from various explants such as flower stalk, nodal segment, cotyledon, shoot, and leaf explants (Rossato et al 2015;Lucchesini et al 2009;Hristova et al 2013;Subhan & Agrawal 2011). Our observation that the best survival of explants occurred at 4.0 and 6.0 mg•dm -3 BAP confirmed the reports of Lucchesini et al (2009) on Echinacea angustifolia.…”

Section: Resultsmentioning

confidence: 99%

In vitro Propagation of Solanecio biafrae and Determination of Genetic Stability of Plantlets Using RAPD and ISSR Markers

Opabode

Akinyemiju

2016

Journal of Horticultural Research

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An efficient and reproducible micropropagation protocol of Solanecio biafrae (Oliv. & Hiern) C. Jeffrey has been developed from nodal stem segments. Shoot development was obtained on Murashige and Skoog (MS) medium supplemented with benzylaminopurine (BAP) alone and in combination with zeatin and 1-naphthaleneacetic acid (NAA). Elongated shoots were rooted in the presence of zeatin or 3-indole-butyric acid (IBA) alone or in combinations. The highest number of explants forming shoots (100%) as well as the highest number of shoots per explant (3.4) and the longest shoots (22 mm) were recorded on medium containing 4.0 mg·dm−3 BAP, 2.0 mg·dm−3 NAA, and 1.0 mg·dm−3 zeatin. About 76% of shoots formed roots on half-strength MS medium free of plant growth regulators. The best root formation (approximately 88%) was recorded on the medium containing 1.0-1.5 mg·dm−3 IBA. The micropropagated shoots with well-developed roots were efficiently acclimatized under greenhouse conditions. The random amplified polymorphic DNA (RAPD) and intersimple sequence repeat (ISSR) amplification products were monomorphic in micropropagated plants and similar to those of mother plant showing their genetic uniformity. This is the first report of micropropagation of S. biafrae, which will facilitate in vitro mass propagation, conservation, and germplasm exchange of this endangered African vegetable.

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“…Reducing the amount of salts in the nutrient medium has been a strategy that is frequently used to induce in vitro rooting (DINIZ, 2014). For example, the use of MS/4 culture medium has provided promising responses for obtaining in vitro adventitious roots in some species, as observed in studies conducted with Azadirachta indica, Lychnophora pinaster, and Pluchea sagittalis (CHATURVEDI et al, 2004;SOUZA et al, 2004;ROSSATO et al, 2015). According to WAREING & PHILLIPS (1982), rooting is favored in more diluted culture media, which may be explained by the cumulative synthesis of endogenous auxins due to the low availability of salts in the culture medium, increasing the metabolic activity of the tissue and, consequently, root induction.…”

Section: In Vitro Rooting and Acclimatizationmentioning

confidence: 99%

Vernonia condensata Baker: an alternative for large-scale seedling production

Almeida¹,

Oliveira²,

Jacobi³

et al. 2020

Cienc. Rural

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The increasing use of Vernonia condensata Baker highlights the importance of developing strategies to reduce the impact of exploitation on nature reserves. The aim of this study was to establish a micropropagation protocol to produce homogenous plants with high phytosanitary quality. Apical, nodal, and internodal segments of plants grown in the field were used for in vitro growth. The segments were disinfected in sodium hypochlorite solution (1.0 and 2.0%) for 15 and 30 minutes and then transferred to Petri dishes containing MS culture medium for 30 days. A completely randomized factorial experiment (3 x 2 x 2) with five replicates was designed. After this period, a completely randomized in vitro multiplication experiment was carried out with six treatments (BAP - 0.0; 0.5; 1.0; 1.5; 2.0; 2.5 mg L-1) and six replicates. The shoots obtained in the best treatment were transferred to flasks with rooting medium (MS, MS/2 or MS/4). The experiment was completely randomized with 12 replicates. Microplants were acclimatized in polyethylene terephthalate (PET) bottles filled with autoclaved topsoil. Our results showed that 40.0% of the nodal segments (immersed in 1.0% sodium hypochlorite for 30 minutes) were adequately disinfected and survived. In the in vitro multiplication experiment, the 0.5 mg L-1 concentration of BAP yielded the highest number of shoots and the best vegetative growth. With regard to the assessed characteristics, MS/4 was the best rooting medium, with 100% survival during acclimatization. This study showed that V. condensata in vitro culture might produce 32,000 seedlings in 7 months.

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Micropropagation of Pluchea sagittalis (Lam.) Cabrera

Cited by 4 publications

References 19 publications

Micropropagação de plantas medicinais no contexto brasileiro: revisão de literatura

Micropropagação de plantas medicinais no contexto brasileiro: revisão de literatura

In vitro Propagation of Solanecio biafrae and Determination of Genetic Stability of Plantlets Using RAPD and ISSR Markers

Vernonia condensata Baker: an alternative for large-scale seedling production

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