Avaliação fitoquímica e atividade antioxidan e, antimutagênica e toxicológica do extrato aquoso das folhas de Ocimum gratissimum L.

Gontijo, Douglas Costa; Fietto, L.C.; Leite, João Paulo Viana

doi:10.1590/1983-084x/12_002

Cited by 14 publications

(8 citation statements)

References 30 publications

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“…Its antinociceptive, intestinal motility antagonistic, antibacterial, and antifungal activities have been proven in previous study (Gontijo et al, 2014).…”

Section: Introductionmentioning

confidence: 69%

“…In addition to EO, the plant also produces saponins, flavonoids, tannins, and cardiac glycosides, as products of their secondary metabolism (Gontijo et al, 2014). Recent studies have demonstrated that clove basil EO has inhibitory effect on the growth of some fungi such as Penicillium and Rhizoctonia (Philippe et al, 2012;Sethi et al, 2013;Rizvi et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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Antifungal activity, yield, and composition of Ocimum gratissimum essential oil

et al. 2017

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ABSTRACT. Ocimum gratissimum L. or clove basil, belongs to the Lamiaceae family, has various desirable uses and applications. Beyond its aromatic, seasoning, and medicinal applications, this plant also has antimicrobial activity. This study was aimed at assessing the antifungal activity, yield, and composition of the essential oil (EO) of O. gratissimum. The species was cultivated in garden beds with dystrophic red latosol soil type containing high organic-matter content. The EO was obtained by hydrodistillation of dried leaves in a modified Clevenger apparatus, followed by determination of its content. Chemical characterization was carried out by gas chromatographymass spectrometry (GC-MS). Microbial activity was assessed using the broth microdilution method, by determining the minimum inhibitory concentration (MIC), in order to compare the antimicrobial effect of EO in 10 isolates-Fusarium oxysporum f. sp tracheiphilum (CMM-0033), F. oxysporum f. sp. cubense (CMM-0813 and CMM-2819), F. oxysporum f. sp lycopersici (CMM-1104), F. solani (CMM-3828), Rhizoctonia solani (CMM-3274), and Macrophomina phaseolina (CMM-2715, CMM-3875, CMM-3615, and CMM-3650). The EO was a highly effective inhibitor of the studied phytopathogenic fungi, with MICs varying from 31.25 to 125 µg/mL. F. oxysporum f. sp lycopersici and R. solani were the most sensitive; both were inhibited at an MIC of 31.25 µg/mL. The EO content in the plant extract was 0.18%. Thirty chemical compounds were detected via GC-MS, with linalool (32.9%) being the major compound followed by 1,8-cineole (21.9%), both oxygenated monoterpenes. It can be concluded that clove basil EO is a highly effective antifungal agent, and therefore, a potential alternative for the control of plant pathogenic diseases.

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“…Its antinociceptive, intestinal motility antagonistic, antibacterial, and antifungal activities have been proven in previous study (Gontijo et al, 2014).…”

Section: Introductionmentioning

confidence: 69%

Section: Introductionmentioning

confidence: 99%

Antifungal activity, yield, and composition of Ocimum gratissimum essential oil

et al. 2017

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“…For Silva et al (2012), the concentration of total phenols, demonstrated a significant result, measured in mg of gallic acid equivalent per gram of sample with 323.95 having +/-1.36 standard deviation, whereas in Kanumfre et al (2017) the value obtained for the species Croton antisyphiliticus Martius was 113.9 ± 1.49 (mg EAG/g), both respectively lower than the present study. In studies on plant extracts, there are statistical correlations between the content of total polyphenols measured by the Folin-Ciocalteu method and antioxidant activity determined by the DPPH test, showing the importance of these secondary metabolites for antioxidant activity (Gontijo et al, 2014).…”

Section: Phenolics Compounds Determinationmentioning

confidence: 99%

Phytochemical characterization, antioxidant potential and antibacterial activity of the Croton argyrophylloides Muell. Arg. (Euphorbiaceae)

et al. 2023

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Croton argyrophylloides Muell. Arg., from the Euphorbiaceae family, popularly known as marmeleiro prateado or sacatinga, is a plant from the Caatinga biome commonly found in Brazil’s northeastern region. The present study aimed to evaluate the antioxidant activity of the species. The phytochemical study was performed through qualitative analysis of chemical constituents and quantitative determination of the total phenol content through the Folin-Ciocalteu test. The qualitative and quantitative antioxidant tests were performed using the DPPH method (2.2 diphenyl-1-picryl hydrazil) and ferric reducing antioxidant power (FRAP). The minimum inhibitory concentration (MIC) was determined by microdilution in 96-well plates. The ethanolic extract of the leaves of C. argyrophylloides manifested antioxidant action in the quantitative DPPH test with a significant bioactivity of 84.70 AAO% in 500 µg/mL, with an EC50 of 236.79. The content of total phenolic compounds was 946.06 mg of gallic acid equivalents/g of sample, and total flavonoids was 58.11 mg of quercetin equivalents/g of sample, the result obtained for FRAP was 15294.44 µM Trolox/g of sample and ABTS was 718 μM Trolox of sample. The prospecting of the chemical constituents of the leaves of C. argyrophylloides revealed the presence of the main compounds that manifests the antioxidant activity and it was proven by the DPPH method that there is antioxidant activity in the analyzed sample, in addition to demonstrating a significant content of phenolic compounds and total flavonoid content in the species, which corroborates the antioxidant activity of the plant sample. The leaf extracts presented growth inhibition halos of 10 and 12 mm upon Staphylococcus aureus ATCC 25923.

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“…This finding fits in the red propolis analyzed in this study since in the evaluation of the chemical composition of the extract, high levels of phenolic compounds and flavonoids, and high antioxidant activity was found. Phenolic compounds have been reported to have genoprotective activity because of their antioxidant activity (Gontijo et al 2014). Thus, the antioxidant activity of red propolis could be the possible explanation for genoprotective effect.…”

Section: Genoprotective Effectmentioning

confidence: 99%

Hepatoprotective and antineoplastic potencial of red propolis produced by the bees Apis mellifera in the semiarid of Rio Grande do Norte, Brazil

et al. 2019

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The objective of this study was to evaluate the hepatoprotective effect of the honey bee Apis mellifera ethanolic extract of the red propolis, obtained in four municipalities of the Rio Grande do Norte semi-arid region, through an in vitro evaluation of the antineoplastic potential in human hepatic carcinoma (HepG2) and normal cell lines (L929), and from the comet assay in hepatic cell lines (ZF-L hepatocytes) to evaluate the genoprotective potential of the extract. The hepatoprotective effect was also evaluated in vivo by the induction of chronic experimental hepatic lesions in rodents (Rattus norvegicus Berkenhout, 1769), Wistar line, by intraperitoneal administration of thioacetamide (TAA) at the dose of 0.2g/kg. The animals were distributed in the following experimental groups: G1 (control), G2 (treated with 500mg/kg ethanolic extract of propolis), G3 (treated with 500mg/kg of ethanolic extract and TAA) and G4 (treated with TAA). All rats were submitted to serum biochemical, macroscopic, histological and stereological biochemical exams of the liver. It was verified the genoprotective effect of red propolis since the mean damages promoted to DNA in cells tested with the extract were significantly lower than the mean of the positive control damage (hydrogen peroxide). The red propolis extract did not present cytotoxic activity to the tumor cells of human liver cancer, as well as to normal ones. The absence of cytotoxicity in normal cells may indicate safety in the use of the propolis extract. The results of the serum biochemical evaluation showed that the serum levels of the aminotransferase enzymes (AST) did not differ significantly between G1, G2 and G3 when compared to each other. G4 showed significant increase in levels compared to the other groups, indicating that the administration of the extract did not cause liver toxicity, as well as exerted hepatoprotective effect against the hepatic damage induced by TAA. The G3 and G4 animals developed cirrhosis, but in G3 the livers were characterized by the presence of small regenerative nodules and level with the surface of the organ, whereas in G4 the livers showed large regenerative nodules. The livers of the G1 and G2 animals presented normal histological appearance, whereas the livers of the G3 animals showed regenerative nodules surrounded by thin septa of connective tissue, and in G4 the regenerative nodules were surrounded by thick septa fibrous connective tissue. The analysis of the hepatic tissues by means of stereology showed that there was no statistical difference between the percentage of hepatocytes, sinusoids, and collagens in G1 and G2. In G3 the percentage of hepatocytes, sinusoids, and collagen did not differ significantly from the other groups. It was concluded that the ethanolic extract of the red propolis exerted a hepatoprotective effect, because it promoted in vitro reduction of the damage to the DNA of liver cells, antineoplastic activity in human hepatocellular carcinoma cell line (HepG2) and did not exert cytotoxic effect in normal cells or was able to reduce liver enzyme activity and the severity of cirrhosis induced by TAA in vivo.

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Avaliação fitoquímica e atividade antioxidan e, antimutagênica e toxicológica do extrato aquoso das folhas de Ocimum gratissimum L.

Cited by 14 publications

References 30 publications

Antifungal activity, yield, and composition of Ocimum gratissimum essential oil

Antifungal activity, yield, and composition of Ocimum gratissimum essential oil

Phytochemical characterization, antioxidant potential and antibacterial activity of the Croton argyrophylloides Muell. Arg. (Euphorbiaceae)

Hepatoprotective and antineoplastic potencial of red propolis produced by the bees Apis mellifera in the semiarid of Rio Grande do Norte, Brazil

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