Taxa de contaminação de testes hematológicos e seus fatores determinantes

Abstract

Objective: Determining the contamination rate of blood cultures and its determining factors. Methods: During a period of six months, were analyzed 564 blood culture samples requested at hospital emergency wards and 46 nurses were inquired. Results: In a period of six months, among a total of 564 requests, 92 blood cultures were contaminated, which corresponds to a contamination rate of 16.31%. The determining factor was the use of low-level sterile technique. Conclusion: The contamination rate of blood cultures is directly related to the procedures used by the nursing staff, and the workload is directly related to errors in the sterile technique of collection. ResumoObjetivo: Conhecer a taxa de contaminação de hemoculturas e os seus fatores determinantes. Métodos: Foram analisadas 564 amostras de hemoculturas requisitadas num serviço hospitalar de urgências e questionados 46 enfermeiras(os) durante um período de seis meses. Resultados: Produziram-se 92 contaminações de hemoculturas de um total de 564 requisições num período de seis meses, o que corresponde a uma taxa de contaminação de 16,31%. O fator determinante foi a utilização de técnica pouco estéril. Conclusão: A taxa de contaminação das hemoculturas está diretamente relacionada aos procedimentos utilizados pelos profissionais de enfermagem e a carga de trabalho está diretamente associada a erros na técnica estéril de coleta.

Characterization of cardiopathic patients in a thoracic pain unit: admission profile

“…contamination were analyzed, as follows: handwashing frequency, use of sterile techniques, contact with the venipuncture zone, number of needles used, respect for drying time, cleaning during the procedure, antiseptic used to clean the vials and skin, compression before or after needle extraction, volume of blood extracted per vial and extraction from existing catheters, when not intended to investigate this site. 13 Using this same protocol, 564 blood cultures were analyzed, where contamination was observed in 92 samples, then representing a percentage of 16.31% of all requested blood cultures. Besides, there was variation in the months studied, with the month of October showing the highest number of contamination (23.85%) and January the month with the lowest number of contamination (9.85%).…”

“…This same study points out that the main factor that causes sample contamination is the workload of the emergency department in which many blood cultures are prescribed, which possibly favors the use of an insufficient sterile technique. 13 Among the most common pathogens found in SSI are CoNS, among them S. epidermidis which, although naturally found in human skin and being the main contaminant of blood cultures, is also capable of promoting major infections, as it is an infectious agent often isolated in ICUs. 14 Regarding the positivity of samples for blood culture collection, studies show that one vial detects 65 to 91% of cases, while two vials show 80 to 99%, and three vials 93% or more.…”

Demand for microbiological cultures and prevalence of microorganisms in a university hospital from the pernambuco state

“…contamination were analyzed, as follows: handwashing frequency, use of sterile techniques, contact with the venipuncture zone, number of needles used, respect for drying time, cleaning during the procedure, antiseptic used to clean the vials and skin, compression before or after needle extraction, volume of blood extracted per vial and extraction from existing catheters, when not intended to investigate this site. 13 Using this same protocol, 564 blood cultures were analyzed, where contamination was observed in 92 samples, then representing a percentage of 16.31% of all requested blood cultures. Besides, there was variation in the months studied, with the month of October showing the highest number of contamination (23.85%) and January the month with the lowest number of contamination (9.85%).…”

“…This same study points out that the main factor that causes sample contamination is the workload of the emergency department in which many blood cultures are prescribed, which possibly favors the use of an insufficient sterile technique. 13 Among the most common pathogens found in SSI are CoNS, among them S. epidermidis which, although naturally found in human skin and being the main contaminant of blood cultures, is also capable of promoting major infections, as it is an infectious agent often isolated in ICUs. 14 Regarding the positivity of samples for blood culture collection, studies show that one vial detects 65 to 91% of cases, while two vials show 80 to 99%, and three vials 93% or more.…”