In vitro evaluation of gingival fibroblasts proliferation and smear layer formation in pre-conditioned root surfaces

Lima, Nayce Melinda Ferreira; Peruzzo, Daiane Cristina; Passador-Santos, Fabrício; Saba-Chufji, Eduardo; Martinez, Elizabeth Ferreira

doi:10.1590/1981-863720160003000043027

Cited by 2 publications

(4 citation statements)

References 16 publications

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“…The results of the current study showed that the cell viability of the fibroblasts attached to roots treated with scaling only was the highest compared with all other groups, and that scaled teeth treated with EDTA or CHX did not enhance cell attachment. This is in agreement with other studies which demonstrated that SRP is the standard requirement for attachment of fibroblasts and that root treatment with EDTA did not enhance cell viability or attachment over SRP alone when measured using either stereomicroscope or scanning electron microscope ( Girotra and Vandana, 2014 , Lima et al, 2016 ). Additionally, other researchers stated that the exposure of fibroblasts to ≥0.02% CHX reduced the survival rate of the cells in vitro ( Liu et al, 2018 ).…”

Section: Discussionsupporting

confidence: 92%

Effect of root conditioning agents hyaluronic acid, EDTA and chlorhexidine on the attachment of human gingival fibroblasts to healthy root surface

Babgi

Alhajaji

Al-Mehmadi

et al. 2021

The Saudi Dental Journal

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Background The gold standard treatment of periodontal diseases is scaling and root planing (SRP). Different adjunctive root conditioning agents such as hyaluronic acid (HA), ethylenediaminetetraacetic acid (EDTA), and chlorhexidine (CHX) have been used with SRP to improve the smear layer removal and the healing of periodontal tissues. The aim of this study was to compare the effect of manual scaling with or without HA, EDTA, or CHX root conditioning on the attachment and viability of human gingival fibroblasts (GF). Methods Fifteen healthy single rooted teeth were co llected and divided randomly into a scaled (n = 12) and non-scaled control group (n = 3). The scaled roots were subdivided equally into four groups; the first group did not receive any chemical treatment, while the remaining groups were treated with the conditioning agents HA or 17% EDTA or 0.2% CHX gel. Gingival fibroblasts were seeded on the top of each root and incubated for 48 h to allow attachment to the roots. The viability of fibroblasts attached to the root surface was assessed using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) cell viability assay. Results The cell viability was the highest in the scaled only group (p = 0.0001) while the lowest was in the scaled with EDTA group (p > 0.05). The scaled group was the highest followed by the HA and CHX groups, while the EDTA group showed the lowest mean value. Conclusion SRP remains the superior method for regaining cell attachment to the root surface, leading to better periodontal health, and adjunctive therapies did not enhance the GF attachment to the root surface beyond the effect of SRP. Further studies are needed to investigate the effect of root conditioning agents on periodontally diseased teeth in vitro and compare them in vivo.

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Section: Discussionsupporting

confidence: 92%

Effect of root conditioning agents hyaluronic acid, EDTA and chlorhexidine on the attachment of human gingival fibroblasts to healthy root surface

Babgi

Alhajaji

Al-Mehmadi

et al. 2021

The Saudi Dental Journal

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“…To the authors’ best knowledge, the effect of antiseptics or antibiotics commonly used in local delivery of a therapeutic agent in the management of peri-implant infections on the migration ability of HGF on zirconia surfaces remains unexplored. However, as noted previously, cellular proliferation was negatively affected as a result of the cytotoxic nature of other common antiseptics, thus implying the inability of cells to migrate in the presence of these antiseptics [ 24 , 25 , 26 , 29 ]. The current study was able to demonstrate an overall positive effect of bergenin on migration in the context of zirconia.…”

Section: Discussionmentioning

confidence: 79%

“…The resazaurin assay for cellular proliferation revealed that bergenin also did not have a significant effect on the rate of cellular proliferation. To date, there is no literature that explores the effect of antiseptics and antibiotics on HGF proliferation in the context of zirconia surfaces; however, several studies demonstrate the negative impact of commonly used oral antiseptics (i.e., CHX, Povidone-Iodine and Hydrogen peroxide) on cellular proliferation and the negative impact of antibiotics that are commonly prescribed for the management of peri-implant infections [ 24 , 25 , 26 , 27 , 28 , 29 ]. Emmadi et al (2008) noted the dose-dependent effect of oral antiseptics, with CHX (0.2%) being more cytotoxic than Povidone-Iodine (1%) [ 24 ].…”

Section: Discussionmentioning

confidence: 99%

“…Adjunct therapies using chemical and pharmacological agents have been employed to aid in the management of mucositis and peri-implantitis through the decontamination of the implant surface and may include antibiotics and antiseptics [ 21 , 22 , 23 ]. Several studies have demonstrated a negative impact on the cellular proliferation of commonly used oral antiseptics (i.e., CHX, Povidone-Iodine and Hydrogen peroxide) and antibiotics that are commonly prescribed for management of peri-implant infections [ 24 , 25 , 26 , 27 , 28 , 29 ]. These results, coupled with the rise of resistance to commonly used antiseptics and antibiotics, makes it imperative that alternative treatments be identified.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Bergenin on Human Gingival Fibroblast Response on Zirconia Implant Surfaces: An In Vitro Study

Xiong,

Miller,

Sharma

2023

JFB

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The poor quality of life associated with the loss of teeth can be improved by the placing of dental implants. However, successful implantation relies on integration with soft tissues or peri-implant inflammatory disease that can lead to the loss of the implant. Pharmacological agents, such as antibiotics and antiseptics, can be used as adjunct therapies to facilitate osseointegration; however, they can have a detrimental effect on cells, and resistance is an issue. Alternative treatments are needed. Hence, this study aimed to examine the safety profile of bergenin (at 2.5 μM and 5 μM), a traditional medicine, towards human gingival fibroblasts cultured on acid-etched zirconia implant surfaces. Cellular responses were analysed using SEM, resazurin assay, and scratch wound healing assay. Qualitative assessment was conducted for morphology (day 1) and attachment (early and delayed), and quantitative evaluation for proliferation (day 1, 3, 5 and 7), and migration (0 h, 6 h and 24 h). The concentrations of bergenin at 2.5 μM and 5 μM did not demonstrate a statistically significant effect with regard to any of the cellular responses (p > 0.05) tested. In conclusion, bergenin is non-cytotoxic and is potentially safe to be used as a local pharmacological agent for the management of peri-implant inflammatory diseases.

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In vitro evaluation of gingival fibroblasts proliferation and smear layer formation in pre-conditioned root surfaces

Cited by 2 publications

References 16 publications

Effect of root conditioning agents hyaluronic acid, EDTA and chlorhexidine on the attachment of human gingival fibroblasts to healthy root surface

Effect of root conditioning agents hyaluronic acid, EDTA and chlorhexidine on the attachment of human gingival fibroblasts to healthy root surface

Effect of Bergenin on Human Gingival Fibroblast Response on Zirconia Implant Surfaces: An In Vitro Study

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