Effect of water temperature and pH on the concentration and time of ozone saturation

Galdeano, Melícia Cíntia; Wilhelm, Allan Eduardo; Goulart, Isabella Borges; Tonon, Renata V.; Freitas-Silva, Otniel; Germani, Rogério; Chávez, Davy William Hidalgo

doi:10.1590/1981-6723.15617

Cited by 21 publications

(14 citation statements)

References 17 publications

(20 reference statements)

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“…The evidence of the great instability of ozone is provided by the large difference between the injected gas content and the saturation concentration. Galdeano et al 64 investigated the effect of temperature and pH of water on the saturation time and gas concentration. At 13.3 mg L −1 bubbled in water, only 26% to 34% of ozone were quantified in the water, and the lowest value occurred with the highest temperature (25°C) and the highest pH (6.0) tested.…”

Section: Discussionmentioning

confidence: 99%

Antibiofilm effect of ozonized physiological saline solution on peri‐implant–related biofilm

Tonon

Panariello

Spolidório

et al. 2020

Journal of Periodontology

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Background Removal of dental plaque and local application of local chemical adjuncts, such as chlorhexidine (CHX), have been used to control and treat peri‐implant disease. However, these methods can damage the surface properties of the implants or promote bacterial resistance. The application of ozone as an adjunctive treatment represents a new approach in the management of peri‐implantitis. Thus, the purpose of this study was to evaluate the antimicrobial effect of ozonized physiological saline solution in different concentrations against oral biofilms developed on titanium surface. Methods Single and multi‐species biofilms of Porphyromonas gingivalis, Fusobacterium nucleatum, and Streptococcus oralis were formed on titanium specimens for 5 days in anaerobic conditions. Biofilms were treated with ozonized saline solution at different concentrations (25, 50, and 80 μg/NmL), for 30 seconds and 1 minute. CHX (0.12%) and saline solution (0.89% NaCl) were used as positive and negative controls, respectively. Bacterial viability was quantified by colony forming units (CFU mL−1), and biofilm images were acquired by confocal laser scanning microscopy (CLSM). Data were analyzed by parametric test (ANOVA) with Tukey post‐hoc test (P < 0.05). Results Ozonized saline solution showed antibiofilm activity at a concentration of 80 μg/NmL for 30 seconds and 1 minute, reducing, mainly, Porphyromonas gingivalis viability, with 2.78 and 1.7 log10 CFU mL−1 of reduction in both single and multi‐species biofilms, respectively, when compared to the control (saline), whereas CHX reduced 1.4 and 1.2 log10 CFU mL−1. Conclusion Ozonized saline solution has antibiofilm activity, with better effect when applied for 1 minute at 80 μg/NmL, being a promising candidate therapy for the treatment of peri‐implant diseases.

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Section: Discussionmentioning

confidence: 99%

Antibiofilm effect of ozonized physiological saline solution on peri‐implant–related biofilm

Tonon

Panariello

Spolidório

et al. 2020

Journal of Periodontology

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“…The treated solution showed near-saturation with ozone after one minute of treatment. However, the ozone treatment in subsequent tests was set at an average temperature of 25 °C with pH = 7 [ 34 , 35 , 36 ]. In order to evaluate the sanitation kinetics, the concentration–time concept was applied, i.e., ozone concentration (C) in mg/L was multiplied by contact-time ( t ) in minutes (C t ) [ 34 , 35 ].…”

Section: Methodsmentioning

confidence: 99%

In Vitro Evaluation of Ozonated Water Treatment on the Viability of Eimeria Oocysts and Giardia Cysts from Water Buffaloes: A Proof-of-Concept Study

Morgoglione

Bosco

Ciucă

et al. 2021

Veterinary Sciences

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The aim of this proof-of-concept study was to evaluate the in vitro effects of ozonated water treatment on the viability of Eimeria oocysts and Giardia cysts isolated from naturally infected water buffaloes. Eimeria oocysts were divided into seven groups of six replicates that were treated with ozonated water at three ozone concentrations (0.5, 1, and 2 mg/L) and two contact times (five and ten minutes), and one group (negative control) that was exposed to non-treated water. Giardia cysts were divided into nine groups of six replicates and were treated with ozonated water at four ozone concentrations (0.1, 0.3, 0.5, and 1 mg/L) and two contact times (one and two minutes), while one group (negative control) was exposed to non-treated water. The results of the ozonated water treatment gave a 33% inhibition of the sporulation of Eimeria oocysts and rendered 96.3% of Giardia cysts non-viable, suggesting that ozonated water treatment could be a promising alternative sanitation technology to common conventional disinfectants for reducing intestinal protozoa infections in water buffaloes; though further in vitro and in vivo tests are needed.

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“…Ozon dapat digunakan dalam bentuk gas atau dilarutkan dalam air dan keberadaan air dapat meningkatkan reaktivitas dari ozon. 19 Penggunaan ozon dalam prosedur asepsis pada praktik dokter gigi khususnya di Indonesia, belum pernah dilakukan. Hal ini dapat disebabkan karena minimnya ketersediaan generator ozon di Indonesia.…”

Section: Pendahuluanunclassified

Pengaruh Paparan Gas Ozon terhadap Jumlah Koloni Jamur Candida albicans

Chusaeni

Wibisono

Skripsa

2021

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Candida albicans is one of the pathogenic fungal species which causes oral infections. One of the methods to minimize the risk of fungal infection is by application of asepsis procedure through gargling with antiseptics. Ozone has high oxidative properties, therefore, it is considered as an antiseptic agent. Plasma Study Center of Diponegoro University has developed an ozone generator machine which opens up an opportunity to identify ozone properties as an antiseptic in controlling the number of fungal colonies, especially Candida albicans. This study was aimed to identify the effects of ozone exposure on the number of fungal colonies of Candida albicans. This was a laboratory experimental study with one-group pretest-posttest design. A total of 32 samples of Candida albicans suspensions were divided into two groups (16 each). Group 1 was not treated with ozonation, while group 2 were ozonated. Then, the two groups were cultured in SDA media using the spread plate method and were incubated for 48 hours. The data were obtained by calculating the number of fungal colonies growing in SDA media. The Wilcoxon test obtained a p-value <0.05, meaning that there were differences in the colony number of Candida albicans before and after ozonation. In conclusion, ozone exposure has a significant effect on the number of fungal colonies of Candida albicans. Further studies are needed adding variables or parameters. Keywords: ozone; fungal colony; Candida albicans Abstrak: Candida albicans merupakan salah satu spesies jamur patogen yang menyebabkan infeksi oral. Salah satu metode untuk meminimalkan risiko infeksi jamur ialah melalui penerapan prosedur asepsis dengan berkumur menggunakan antiseptik. Ozon memiliki kemampuan oksidasi tinggi sehingga dipertimbangkan untuk menjadi bahan antiseptik. Pusat Penelitian Plasma Universitas Diponegoro telah mengembangkan mesin generator ozon yang membuka peluang untuk mengetahui kemampuan ozon sebagai antiseptik dalam mengendalikan jumlah koloni jamur terutama Candida albicans. Penelitian ini bertujuan untuk mengetahui adanya pengaruh paparan gas ozon terhadap jumlah koloni jamur (Candida albicans). Jenis penelitian ialah eksperimental laboratorik dengan one group pretest-posttest design. Sebanyak 32 sampel suspensi jamur Candida albicans dibagi menjadi dua kelompok (masing-masing 16 sampel). Kelompok 1 tidak dilakukan ozonasi dan kelompok 2 dilakukan ozonasi kemudian kedua kelompok dikultur dalam media SDA dengan metode spread plate dan diinkubasi 48 jam. Data diperoleh dengan menghitung jumlah koloni jamur yang tumbuh pada media SDA. Hasil uji statistik Wilcoxon mendapatkan nilai p<0,05 yang berarti terdapat perbedaan jumlah koloni jamur (Candida albicans) sebelum dan setelah pemberian gas ozon. Simpuolan penelitian ini ialah paparan gas ozon berpengaruh secara bermakna terhadap jumlah koloni jamur (Candida albicans). Penelitian lanjutan diperlukan dengan penambahan variabel atau parameter.Kata kunci: gas ozon; koloni jamur; Candida albicans

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Effect of water temperature and pH on the concentration and time of ozone saturation

Cited by 21 publications

References 17 publications

Antibiofilm effect of ozonized physiological saline solution on peri‐implant–related biofilm

Antibiofilm effect of ozonized physiological saline solution on peri‐implant–related biofilm

In Vitro Evaluation of Ozonated Water Treatment on the Viability of Eimeria Oocysts and Giardia Cysts from Water Buffaloes: A Proof-of-Concept Study

Pengaruh Paparan Gas Ozon terhadap Jumlah Koloni Jamur Candida albicans

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