Genotypes distribution of human papillomavirus in cervical samples of Ecuadorian women

Muentes, Gustavo David García; Rodriguez, Lindsay Karen García; Galárraga, Ramiro Israel Burgos; Carpio, Franklin Almeida; Cabezas, J.

doi:10.1590/1980-5497201600010014

Cited by 16 publications

(14 citation statements)

References 16 publications

(11 reference statements)

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“…We found HPV16 in 19 (41.30%), HPV18 in 9 (19.57%), and HPV45 in 1 (2.17%) of HPV positive samples. Human papillomavirus16 and HPV18 had the highest frequencies in cervical cancer (22,41), which is similar to our result. Another result showed that the frequencies of HPV35 and HPV31 were the highest (32), which is inconsistent with our result.…”

Section: Discussionsupporting

confidence: 92%

The Prevalence and Genotype Distribution of Cervical Human Papillomavirus DNA in Women with Normal Cytology in North of Iran

Daneshvar

Haghshenas

Majleci

et al. 2016

Jundishapur J Microbiol

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Background: Cervical cancer is a leading cause of mortality in women. Infection with high-risk human papillomavirus (HPV) is recognized as an important etiological factor for the development of cervical cancer. On the other hand, cervical cancer screening can detect abnormal cervical cells and diagnose cervical cancer in the early stages. Objectives: This study was performed to determine and analyse the distribution of HPV genotypes in normal cervical tissues, using polymerase chain reaction in Mazandaran province in North of Iran. Methods: This cross-sectional study was performed during 2012 -2015. Tissue samples from the cervix were collected from 450 women, referring to a healthcare centre in Mazandaran province, Iran. Pap smear was used to monitor any abnormalities or unusual findings. Also, GP5 + / GP6 + primers were applied for the detection of HPV DNA in the specimens. Afterwards, positive samples were selected to perform high-risk HPV genotyping, using the HPV HCR DNA genotyping kit. Results: In this study, 431 (95.78%) samples were found to be normal on the Pap smear test, while 19 (4.22%) samples were abnormal. We found that the rate of positive HPV DNA was 10.22% in the tissue cervix samples. Human papillomavirus DNA was detected in 8.12% (35 of 431) of the normal tissues and 57.89% (11 of 19) of the abnormal tissues. From the 46 HPV DNA positive samples, 19 (41.30%) were HPV16, 9 (19.57%) of which were HPV18 and 1 (2.17%) was HPV45. In this study, the highest prevalence of HPV DNA was found in women within the age range of 20-24 years, which constitutes 20 (28.17%) of the HPV DNA positive samples. Conclusions: Cervical cancer screening, comprising of both Pap test and HPV testing, is an essential part of the routine health care for women as it can facilitate the detection of abnormal cervical cells. HPV infection can produce abnormal Pap test results; also, certain types of HPV are associated with cancer. Therefore, it is important to diagnose abnormal cervical tissues before the development of cervical cancer.

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Section: Discussionsupporting

confidence: 92%

The Prevalence and Genotype Distribution of Cervical Human Papillomavirus DNA in Women with Normal Cytology in North of Iran

Daneshvar

Haghshenas

Majleci

et al. 2016

Jundishapur J Microbiol

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“…However, Salehi-Vaziri et al reported that the most common HPV genotypes in Iranian women were HPV 16 and 53 [16]. García Muentes et al reported that the most common HPV genotypes in Ecuadorian women were HPV 16 and HPV 33 [17].…”

Section: Discussionmentioning

confidence: 99%

HPV Genotypes of Senegalese Women Attending Radiotherapy

Soumboundou¹,

Thiam²,

Doh³

et al. 2018

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Objectives: This work was carried out in order to determine the profile of different HPV genotypes in a population of women attending radiotherapy (RT). Material and Methods: This is a retrospective study. Sixty paraffin blocks were chosen by simple random method upon 1015 blocks of cervix cancer diagnosed in Senegal's public hospitals laboratories of anatomopathology. The strains of HPV genotyping were done with polymerase chain reaction (PCR) multiplex using primers MY09-MY11, GP5+ and GP6+. Results: Among the sixty examined blocks, DNA extraction was unsatisfactory in three cases. All women were infected with HPV high risk (HR). The different HPV genotypes isolated were HPV 16 (49/96%), 18 (8/16%), 35 (7/14%), 45 (4/8%), and 58 (2/4%). It was a single infection in 36 cases (70, 60%), co-infection in 11 cases (21, 60%) and a multi-infection in 4 cases (7, 80%). Conclusion: The prevalence of HPV found in our series is comparable to that found in the world. To complete this study, a large population of Senegalese patients with uterine cervix cancer (CC) and HPV genotype, is needed to determine if HPV genotypes could be considered as a prognostic marker in patients with uterine CC after RT.

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“…Some authors suggest that the presence of these genotypes (high-grade lesions) would be more likely to progress to cervical cancer than other HPV types; this is due to the oncogenic potential of these genotypes [41]. …”

Section: Discussionmentioning

confidence: 99%

Prevalence of High-Risk Genotypes of Human Papillomavirus: Women Diagnosed with Premalignant and Malignant Pap Smear Tests in Southern Ecuador

Aguilar

González

Rodriguez

et al. 2017

Infectious Diseases in Obstetrics and Gynecology

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Human papillomavirus (HPV) is the primary infectious agent for the development of cervical cancer, although the presence of the virus alone is insufficient for viral development and proliferation; this can be attributed to the increase in potential oncogenic risk, along with other risk factors. In the present investigation, the prevalence of high-risk HPV was determined from samples of premalignant or malignant cervical cytology in women from the southern region of Ecuador. The kit we used was able to detect genotypes 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, and 59. In addition, 64.5% of the analyzed samples were positive for HPV, with genotypes 16 and 18 being the most prevalent (16 was detected in 148 samples and 18 in 108). Genotypes 58 and 51 were the third most frequent simple and multiple infections, respectively. The data are very similar to those obtained worldwide, suggesting that the strategy of sex education, and the use of vaccines as primary prevention agents, could significantly decrease the incidence and mortality rate of cervical cancer in the southern region of Ecuador.

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Genotypes distribution of human papillomavirus in cervical samples of Ecuadorian women

Abstract: Viral genotypes different from HPV 16 and HPV 18 are frequently detected among Ecuadorian women. The overall prevalence of HPV resulted higher than the one reported in other South American countries with a greater burden in the second and third decades of life.

Cited by 16 publications

References 16 publications

The Prevalence and Genotype Distribution of Cervical Human Papillomavirus DNA in Women with Normal Cytology in North of Iran

The Prevalence and Genotype Distribution of Cervical Human Papillomavirus DNA in Women with Normal Cytology in North of Iran

HPV Genotypes of Senegalese Women Attending Radiotherapy

Prevalence of High-Risk Genotypes of Human Papillomavirus: Women Diagnosed with Premalignant and Malignant Pap Smear Tests in Southern Ecuador

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