Isolating the Effects of Mg2+, Mn2+ and Sr2+ Ions on Osteoblast Behavior from those Caused by Hydroxyapatite Transformation

Silva, Leila Melo da; Tavares, Débora dos Santos; Santos, Euler Araújo dos

doi:10.1590/1980-5373-mr-2020-0083

Cited by 15 publications

(6 citation statements)

References 55 publications

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“…Magnesium plays a key role in bone mineralization and bone as it improves the osteointegration and osteoblasts activity and accelerates bone ingrowth, enhancing integrin ligand binding and protecting cells by oxidative stress [ 105 , 106 ]. The presence of Mg 2+ ions on the apatite surface results in an increase in the amount of water molecules adsorbed on the surface, affecting cell attachment and differentiation [ 107 , 108 ].…”

Section: Translation Of the Biomimetic Concept To 3d Scaffold Develop...mentioning

confidence: 99%

“…In this context, some studies have investigated the solubility behavior of ion-doped and multi-doped apatites, considering that each ion has limited solubility in the apatite lattice, thus resulting only in partial substitution. Although the insertion of foreign bioactive ions, such as Mg 2+ , Sr 2+ , and CO 3 2− ions in the apatitic structure causes a general enhancement of the overall solubility in synthetic biological fluids or in cell culture media [ 86 , 90 , 105 , 129 , 130 , 131 , 132 , 133 , 134 ], the solubility behavior of doped and multi-doped apatites can be quite difficult to predict. In fact, the extent of ion substitution in apatites is subjected to various and interlacing key aspects and the co-existence of different foreign anions and cations can influence substantially the solubility behavior of the resulting matrix.…”

Section: Translation Of the Biomimetic Concept To 3d Scaffold Develop...mentioning

confidence: 99%

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Design Strategies and Biomimetic Approaches for Calcium Phosphate Scaffolds in Bone Tissue Regeneration

et al. 2022

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Bone is a complex biologic tissue, which is extremely relevant for various physiological functions, in addition to movement, organ protection, and weight bearing. The repair of critical size bone defects is a still unmet clinical need, and over the past decades, material scientists have been expending efforts to find effective technological solutions, based on the use of scaffolds. In this context, biomimetics which is intended as the ability of a scaffold to reproduce compositional and structural features of the host tissues, is increasingly considered as a guide for this purpose. However, the achievement of implants that mimic the very complex bone composition, multi-scale structure, and mechanics is still an open challenge. Indeed, despite the fact that calcium phosphates are widely recognized as elective biomaterials to fabricate regenerative bone scaffolds, their processing into 3D devices with suitable cell-instructing features is still prevented by insurmountable drawbacks. With respect to biomaterials science, new approaches maybe conceived to gain ground and promise for a substantial leap forward in this field. The present review provides an overview of physicochemical and structural features of bone tissue that are responsible for its biologic behavior. Moreover, relevant and recent technological approaches, also inspired by natural processes and structures, are described, which can be considered as a leverage for future development of next generation bioactive medical devices.

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Section: Translation Of the Biomimetic Concept To 3d Scaffold Develop...mentioning

confidence: 99%

Section: Translation Of the Biomimetic Concept To 3d Scaffold Develop...mentioning

confidence: 99%

Design Strategies and Biomimetic Approaches for Calcium Phosphate Scaffolds in Bone Tissue Regeneration

et al. 2022

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“…Magnesium-doped HA (MgHA) enhances the solubility and biodegradability in physiological media and affects positively the biological effect by improving the osteointegration and osteoblasts activity, and accelerates bone ingrowth [ 12 ]. Mg doping enhances integrin-ligand binding and appears to protect the cells by oxidative stress [ 13 ].…”

Section: Bioactive Ion-doped Nanohydroxyapatitesmentioning

confidence: 99%

Nature-Inspired Unconventional Approaches to Develop 3D Bioceramic Scaffolds with Enhanced Regenerative Ability

et al. 2021

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Material science is a relevant discipline in support of regenerative medicine. Indeed, tissue regeneration requires the use of scaffolds able to guide and sustain the natural cell metabolism towards tissue regrowth. This need is particularly important in musculoskeletal regeneration, such as in the case of diseased bone or osteocartilaginous regions for which calcium phosphate-based scaffolds are considered as the golden solution. However, various technological barriers related to conventional ceramic processing have thus far hampered the achievement of biomimetic and bioactive scaffolds as effective solutions for still unmet clinical needs in orthopaedics. Driven by such highly impacting socioeconomic needs, new nature-inspired approaches promise to make a technological leap forward in the development of advanced biomaterials. The present review illustrates ion-doped apatites as biomimetic materials whose bioactivity resides in their unstable chemical composition and nanocrystallinity, both of which are, however, destroyed by the classical sintering treatment. In the following, recent nature-inspired methods preventing the use of high-temperature treatments, based on (i) chemically hardening bioceramics, (ii) biomineralisation process, and (iii) biomorphic transformations, are illustrated. These methods can generate products with advanced biofunctional properties, particularly biomorphic transformations represent an emerging approach that could pave the way to a technological leap forward in medicine and also in various other application fields.

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“…The positive effects of Mg 2+ in terms of bone and dentin generation are witnessed by their diffused presence as dopants in hydroxyapatite and other calciumphosphate based systems. [23,24]. Magnesium oxide (MgO), another kind of inorganic filler, with enhanced biocompatibility and non-toxic biological activity, became a research hotspot of polymer modification [25].…”

Section: Introductionmentioning

confidence: 99%

Part I: Dentinogenic Effect of Magnesium Oxide on Normal Human Dental Pulp Cells: An In Vitro Study

Salem¹

2021

Madridge J Dent Oral Surg

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Due to a limited ability to achieve self-repair and regeneration as well as lack of effective therapeutic options, degeneration and injury of the pulp-dentin complex may result in severe consequences. Magnesium-based biomaterials might provide an innovative therapeutic potential to substantially enhance regeneration of dental tissues. Magnesium (Mg 2+ ) has been considered for its potential ability to accelerate proliferation and differentiation of human osteoblasts. However, to date, magnesium oxide (MgO) and its dentinogenic effects on human dental pulp cells (HDPCs) has not been investigated. This study was designed to evaluate the stimulatory effect of different concentrations of MgO on dentinogenesis of HDPCs. HDPCs were cultured with 0.5 mM, 1 mM, 2 mM, 4 mM, 8 mM concentrations of supplemental MgO, 0 mM as the negative control group, lignin sulfonic acid sodium salt and xanthan gum as the vehicle control groups. Cell attachment efficiency was assessed at 16 h. Proliferation rate was evaluated at 3, 7, 10, 14 days. Both attachment efficiency and proliferation rate were assessed by crystal violet staining. Cell viability was determined by activity of mitochondrial dehydrogenase enzyme. Alkaline phosphatase (ALP) activity was assessed using fluorometric assay at 7, 10, and 14 days. Mineralization of cultures was measured by Alizarin Red staining. Statistical analysis was performed using multi-way ANOVA with Wilks' lambda test. Higher cell attachment efficiency was shown with 0.5 mM at 16 hours compared to negative control (P<0.001). Cells with 0.5 mM supplemental MgO showed significantly higher proliferation rates than negative and vehicle controls at 7, 10, and 14 days (P<0.001). Higher levels of ALP activity and mineralization were also observed in 0.5 mM supplemental MgO at 10, and 14 days (P<0.001). In conclusion, optimal MgO (0.5 mM) group significantly upregulated HDPCs attachment, proliferation, ALP activity, odontogenic differentiation and mineralization. Magnesium oxide containing biomaterials could be a potential novel material for pulp and dentin repair in regenerative endodontics.

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Isolating the Effects of Mg2+, Mn2+ and Sr2+ Ions on Osteoblast Behavior from those Caused by Hydroxyapatite Transformation

Cited by 15 publications

References 55 publications

Design Strategies and Biomimetic Approaches for Calcium Phosphate Scaffolds in Bone Tissue Regeneration

Design Strategies and Biomimetic Approaches for Calcium Phosphate Scaffolds in Bone Tissue Regeneration

Nature-Inspired Unconventional Approaches to Develop 3D Bioceramic Scaffolds with Enhanced Regenerative Ability

Part I: Dentinogenic Effect of Magnesium Oxide on Normal Human Dental Pulp Cells: An In Vitro Study

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