Fish Bone as a Source of Raw Material for Synthesis of Calcium Phosphate

Abstract

Fish bone is rich in calcium carbonate, which makes it an alternative source of low cost calcium carbonate for the synthesis of calcium phosphate bioceramic for use in bone regeneration. The calcium phosphate bioceramic was prepared by a wet precipitation method with acid and base reactions. The synthesized bioceramic was characterized in terms of X-ray diffraction (XRD), scanning electron microscopy (SEM/EDS), thermogravimetric analysis (TGA), Fourier transform infrared spectroscopy (FTIR), average crystallite size and refinement by the Rietveld method for the quantification of crystalline phases. The results indicated the formation of a biphasic calcium phosphate bioceramic comprising 67.6 % of β-calcium pyrophosphate (β-CPP) and 32.1 % of β-tricalcium phosphate (β-TCP). This biphasic calcium phosphate bioceramic synthesized using fish bone waste presented nanostructured nature with an average crystallite size of 69.58 nm, which is very promising for biomedical applications.

Formulation, characterisation and sensory evaluation of Bio‐Cal instant jelly fortified with chicken bone‐based nano‐biogenic hydroxyapatite

“…Logically, the biological sources of natural HAp commonly exhibit inconsistency and irregularity in their shape. Corrêa & Holanda (2019) reported that the morphological SEM-based images of nano particles, extracted from fish bone HAp, highlighted the agglomerated and irregular particle shape. Another scientific research reported by Barua et al (2019) demonstrated that the SEM images of HAp extracted from chicken bone underlined the agglomerated and irregular shape of particles of crystals.…”

Histopathological Analysis and Comparison between Experimentally Fabricated Hydroxyapatite from Nile Tilapia Bone and Mineral Trioxide Aggregate as A Direct Pulp Capping Agents on Dog Pulp (In Vivo Study)

“…Fish waste such as bone, skin, head and scale contain high biological components that can be turned into useful products [25]. The extraction process of hydroxyapatite (HA) from natural resources are better than synthetic for its biocompatibility, using uncomplicated method on production, and low cost [26]. It has been demonstrated that fish sources are safe and present low risks of disease transmission [27].…”

Autoclaving and Alkaline Hydrolysis Effects on the Particle Size and Solubility of Grouper (<I>Epinephelus</I> sp.) Nano-calcium Powder in <I>In Vitro</I> Gastrointestinal Tract Simulation

“…The chemical structure of the powder samples was determined using an FTIR instrument (Nicolet type iS10, Thermo Fisher Scientific, Massachusetts, USA). All samples were prepared using KBr pellets, and spectra were acquired in the 400-1400 cm -1 middle infrared range (Corrêa and Holanda, 2019).…”