The Brazilian Amazon rainforest is rich in natural resources, which are widely used in areas such as food and health, especially for local populations. Couepia edulis Prance is a tree from the Amazon, consisting of fruits called agouti nut, which its use as a herbal medicine is widespread in the Amazon region. That said, the aim of this article was to verify the cytotoxicity of Agouti nut oil. For this, the nutritional characteristics, physical-chemical and bioactive properties of agouti nut oil were verified, for subsequent cytotoxicity bioassay. In the centesimal composition it was identified: Caloric Value 658.84± 0.02; Proteins 10.01 ± 0.03; Ash 2.34 ± 0.04; Lipids 61.20 ± 0.15; Carbohydrates 17.00 ± 0.20; Fibers 29.74 ± 0.64. In the fatty acid profile: arachidic (0.42%), palmitoleic (1.02%), stearic (7.11%), linoleic (14%), palmitic (29.20%), oleic (35.01%). In the cytotoxicity assay at different extract concentrations (100; 50; 6.25 and 1.56 µg.mL-1) cell viability of 78.60 to 90.20% was identified. We conclude that the almond and the oil of the Agouti nut at the nutritional and physical-chemical level were within the food standards for consumption, with a high content of total fibers.