ADIÇÃO DE HIDROXITOLUENO BUTILADO (BHT) NO DILUIDOR ACP-106c PARA CONGELAÇÃO DE SÊMEN CANINO

Silva, Herlon Victor Rodrigues; Nunes, Thalles Gothardo Pereira; Filho, Antônio Cavalcante Mota; Pinto, José Nicodemos; Silva, Alexandre Rodrigues; Silva, Lúcia Daniel Machado da

doi:10.1590/1809-6891v19e-45896

Cited by 2 publications

(5 citation statements)

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“…The effects of cryopreservation on sperm head morphometry have been reported in human (Maree et al, 2010), bovine (Valverde et al, 2016), ovine (Ramon et al, 2013), caprine (Hidalgo et al, 2007), equine (Arruda et al, 2002), canine (Silva et al, 2018) species and in wild boars (García-Herreros et al, 2008). All of these studies have shown that the sperm submitted to cryopreservation undergoes a significant decrease in the morphometric dimensions of the head, which are justified by possible damage or loss of the acrosome (Rijsselaere et al, 2004;Núñez-Martinez et al, 2007b); super condensation of chromatin (Núñez-Martinez et al, 2007b) and osmotic changes associated with excessive cell dehydration (Arruda et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

“…After, the samples were packaged in French straws of 0.25 mL and placed horizontally on a freezing ramp at a height of 5 cm from the liquid nitrogen slide, for 5 minutes. The straws containing the samples were stored in cryogenic cylinders (Silva et al, 2018).…”

Section: Semen Collection Freezing and Thawingmentioning

confidence: 99%

“…The thawing process was carried out after a minimum of 7 days of freezing. The protocol consisted of removing the frozen straws from the cylinder and immersing them in a water bath programmed at 37 ºC for 1 minute (Silva et al, 2018). The sperm samples were transferred to 2 mL plastic tubes, heated to 37 ºC and, immediately submitted to sperm evaluation.…”

Section: Semen Collection Freezing and Thawingmentioning

confidence: 99%

“…Semen cryopreservation is advantageous because it protects the genetic potential of males, maintaining the fertilizing capacity of gametes even after the death of the animal. Besides that, it enables the rapid spread of gametes to different regions of the planet (Silva et al, 2018). However, it is emphasized that in the cryopreservation process, gametes can pass through structural and functional changes, such as the rupture of plasma membranes and DNA fragmentation (Sieme et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Sperm quality and morphometry characterization of cryopreserved canine sperm in ACP-106c or TRIS

et al. 2022

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Morphological sperm evaluation supported by the morphometry can be used in the determination of the seminal quality and in the investigation of potential extenders. Although there are studies comparing TRIS and ACP extenders, there are no comparative studies between them for the computerized assisted semen analysis (CASA), sperm viability, membrane functionality and sperm morphometry parameters of cryopreserved canine semen. Hence, we aimed to evaluate the effects of ACP-106c and TRIS on post-freezing canine sperm quality. Five dogs were submitted to semen collection twice with one-week interval. The semen was evaluated within the parameters: total motility, vigor, concentration, viability, plasma membrane functionality, morphology and morphometry. In the morphometric evaluation, the morphologically normal sperm was measured as: length, width, area and perimeter of the head and the midpiece, tail length and total length. The parameters of ellipticity, elongation, regularity and roughness were determined. Then, the semen was divided into two aliquots that were diluted in TRIS or ACP-106c, with the addition of egg yolk and glycerol. The diluted semen was refrigerated and frozen. The thawed samples were evaluated. Total motility, viability, sperm membrane functionality and normal morphology reduced after thawing in both extenders (morphology reduced from 89.60 ± 1.3% to 84.40 ± 1.8 and 84.60 ± 1.1% in TRIS and ACP-106c, respectively). However, it did not differ between TRIS and ACP-106c. In the ACP-106c the sperm head defects in cryopreserved semen were higher compared to fresh semen (P < 0.05). For all the morphometric parameters evaluated, there were no differences between fresh and cryopreserved samples (3.70 ± 0.4% vs. 2.30 ± 0.5%). In kinetics, with an interval of one week statistical differences between the extenders were found only in the parameters ALH and LIN (P < 0.05). Regardless of the extender, there were no changes in the morphometric parameters of sperm after thawing.

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Section: Discussionmentioning

confidence: 99%

Section: Semen Collection Freezing and Thawingmentioning

confidence: 99%

Section: Semen Collection Freezing and Thawingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Sperm quality and morphometry characterization of cryopreserved canine sperm in ACP-106c or TRIS

et al. 2022

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“…Segundo Silva et al (2018), a microscopia eletrônica de transmissão é uma ferramenta utilizada para determinar o tamanho e a forma das estruturas orgânicas (biológicas) e inorgânicas. Baseia-se na interação dos elétrons incidentes sobre a matéria.…”

Section: Discussionunclassified

Avaliação da viabilidade espermática de sêmen caprino criopreservado em meio ACP-101c e TRIS acrescido de gema de ovo de Numida meleagris

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Um meio diluente ideal para a criopreservação seminal deve suprir as células espermáticas com energia, proteção e manutenção de um ambiente adequado à sua sobrevivência. Objetivou-se avaliar a integridade in vitro do sêmen caprino criopreservado em meio ACP-101c® associado à gema de ovo de Numida meleagris, por meio de duas técnicas de análise individual da célula espermática. Foram colhidos 15 ejaculados de cinco caprinos, com auxílio de uma vagina artificial. Os ejaculados foram reunidos em um pool, e dividido em 12 grupos, sendo dois grupos controles: GC1 TRIS, com adição 2,5% da gema de ovo de Gallus gallus domesticus GOGD, GC2 ACP-101c®, com adição 2,5% da gema de ovo de Gallus gallus domesticus GOGD e dez grupos experimentais GE, contendo a adição da gema de ovo de Numida meleagris. Seguidamente, as alíquotas foram envasadas em palhetas francesas de 0,25 mL e congeladas com auxílio do aparelho TK3000® e armazenadas em nitrogênio líquido. Amostras foram descongeladas após sete dias e avaliadas quanto à integridade do DNA e quanto a sua ultraestrutura individual, por meio do teste cometa e microscopia eletrônica de transmissão, respectivamente. Nos resultados obtidos pelo teste cometa, não foi evidenciado diferença estatística quanto ao comprimento da cauda, entre os grupos TRIS acrescido de GONM, nas concentrações de 2,5%, 5% e 10% em relação ao grupo controle TRIS 2,5% de GOGD. Também não houve diferença estatística quanto ao percentual de fragmentação de DNA na cauda, nos grupos TRIS com 2,5%; 5%; e 20% de GONM em comparação ao grupo controle TRIS 2,5% GOGD (P>0,05). Os grupos ACP® com 10%, 15% e 20% de GONM apresentaram maior comprimento de cauda quando comparados aos grupos ACP com 2,5% e 5% de GONM e grupo controle (ACP® 2,5% de GOGD). Na análise ultraestrutural o grupo ACP® com 10% de GONM, destacou-se com melhor integridade celular frente aos demais grupos, inclusive, frente às amostras avaliadas do grupo controle. Dessa forma, conclui-se que a gema de ovo de Numida meleagris, como crioprotetor externo de membrana, acrescida aos diluentes ACP-101c® ou TRIS, pode reduzir os danos causados durante o processo de criopreservação do sêmen caprino.

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ADIÇÃO DE HIDROXITOLUENO BUTILADO (BHT) NO DILUIDOR ACP-106c PARA CONGELAÇÃO DE SÊMEN CANINO

Cited by 2 publications

References 24 publications

Sperm quality and morphometry characterization of cryopreserved canine sperm in ACP-106c or TRIS

Sperm quality and morphometry characterization of cryopreserved canine sperm in ACP-106c or TRIS

Avaliação da viabilidade espermática de sêmen caprino criopreservado em meio ACP-101c e TRIS acrescido de gema de ovo de Numida meleagris

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