Evaluation of the antioxidant potential of Copaifera multijuga in Ehrlich tumor-bearing mice

Abstract

Copaifera multijuga, commonly known as copaiba, is popularly used in the form of tea for various conditions due to the presence of antioxidant substances in its composition, which protect cells against damage caused by free radicals. Its oleoresin is also used as an anti-inflammatory and antitumoral agent. The present study investigated the antioxidant effect of the ethanolic extract of copaiba stem bark on Swiss mice inoculated with solid Ehrlich tumors. Mice were inoculated subcutaneously with 1x106 Ehrlich’s tumor cells and treated via gavage with ethanolic extract of copaiba for thirty days, with doses varying between 100 and 200 mg kg-1. Biochemical analyses of enzymatic antioxidants [superoxide dismutase (SOD), catalase (CAT), glutathione-S-transferase (GST)], non-enzymatic antioxidants [reduced glutathione (GSH) and ascorbic acid (ASA)], substances reactive to thiobarbituric acid (TBARS) and protein carbonylation (carbonyl) in different tissues were significantly affected. The extract administered at 200 mg kg-1 presented higher antioxidant capacity in the liver, increased CAT, GST, GSH and decreased TBARS, as well as increased CAT activity and protein carbonylation in brain tissue. The results showed that the copaiba extract was able to reverse the oxidative stress caused by solid Ehrlich tumor, probably due to the presence of antioxidant compounds, and had potential antineoplasic effect after a 30-day treatment.

Natural killer activity of the spleen cells of Ehrlich tumor-bearing mice treated with Copaifera multijuga extract

“…C. multijuga bark ethanolic extract also increased the antioxidant capacity of the liver, increased catalase, reduced glutathione and glutathione-S-transferase activities, and decreased the levels of lipid peroxidation [19]. Results of the present study corroborate previous data [9,19], showing that the immunomodulatory effect of C. multijuga on Ehrlich tumor-bearing mice is also a consequence of NK cell activity stimulation.…”

“…Previous study by our group demonstrated that the ethanolic extract of C. multijuga bark can affect Ehrlich tumor cells, reducing their viability in vitro as well as their development in vivo, reducing tumor mass to 45%, and increasing the production of IL-12p70, TNF-α, and IFN-γ in Con A or SAC-stimulated spleen cell culture supernatants [9]. C. multijuga bark ethanolic extract also increased the antioxidant capacity of the liver, increased catalase, reduced glutathione and glutathione-S-transferase activities, and decreased the levels of lipid peroxidation [19]. Results of the present study corroborate previous data [9,19], showing that the immunomodulatory effect of C. multijuga on Ehrlich tumor-bearing mice is also a consequence of NK cell activity stimulation.…”

Evaluation of the antitumor and antioxidant effects of jatobá (Hymenaea courbaril) extracts / Avaliação do efeito antitumoral e antioxidante de extratos do jatobá (Hymenaea courbaril)

“…In fact, the methanolic fraction of the seed of Jatobá restored the activity of catalase, glutathione-S-transferase, and ascorbic acid 20 . Although we did not investigate the antioxidant enzymes, the analyses presented here provide some knowledge regarding the behavior of this part of the plant in relation to the Brazilian Journal of Development, Curitiba, v.7, n.12, p. 116001-116018 dec. 2021 parameters analyzed and revealed that the greatest effects were observed up to 14 days of treatment, unlike the studies conducted by Cunha et al 32 which assessed different concentrations of copaiba stem bark extract in animals with Ehrlich tumor for 30 days.…”

UHPLC-HRMS/MS on untargeted metabolomics: a case study with Copaifera (Fabaceae)

“…15,26 The presence of compounds 13 with C. lucens and C. venezuelana gives preliminary evidences of their antioxidative activity and an antineoplastic effect against Ehrlich ascites carcinoma, since this diterpene have already being reported as one of the responsible metabolites for C. multijuga extracts bioactivity. 18 The occurrence of condensed tannins only in barks indicate such compounds as chemical markers of this organ within the genus and can be considerate to compose a set of chemical parameters to evaluate quality control of herbal products or even identication of the Copaifera genus. 7,20,27 Blue and cyan group also emphasized a relationship among species.…”

“…However, copaíba extracts are not as famous as the oilresins, and present substantially few scientic exploitations. [16][17][18] Several review papers have already described copaíba oilresin composition and pharmacological properties. 19,20 Nonetheless, the composition of the extracts from Copaifera vegetative and reproductive organs (e.g.…”