Métodos Diagnósticos Para Os Patógenos Alimentares: Campylobacter Sp., Salmonella Sp. E Listeria Monocytogenes

Andrade, Renata Barbosa; Gemelli, Tanise; Onder, L. P. D.; Cristina, Karla; Brito, Thales de; Barboza, Antônio Augusto Lazarini; Brito, Benito Guimarães de

doi:10.1590/1808-1657v77p7412010

Cited by 9 publications

(3 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…According to the analytical profile of RNLVISA, in 2019, 11 laboratories distributed in five regions of Brazil carried out research on L. monocytogenes in food, with the test characterized as microbiological or research for toxins (Agência Nacional de Vigilância Sanitária, 2018). These data contrast with the initiative to use techniques based on molecular biology in the detection of L. monocytogenes, which are useful during investigations in the food industry that require fast and sensitive methodologies to monitor the pathogen's incidence in production (Andrade et al, 2010).…”

Section: Laboratories Of the National Health Surveillance Systemmentioning

confidence: 79%

“…Traditional methods require long and laborious steps, which are against the urgency of control during food outbreaks. Thus, techniques based on polymerase chain reaction (PCR) can be used to identify pathogens (Andrade et al, 2010). To carry out PCR-based methodologies, it is necessary to carry out the steps of extraction of deoxyribonucleic acid (DNA), amplification of the target gene, and separation of the amplified products in agarose gel after the stage of cultivation in selective media for Listeria (Chen et al, 2017).…”

Section: Identification Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Listeria monocytogenes: challenges of microbiological control of food in Brazil

et al. 2022

View full text Add to dashboard Cite

Listeria monocytogenes is a gram-positive bacterium that can survive in food production environments and food products. This microorganism is associated with listeriosis, a serious infection caused by the consumption of contaminated food. The aim of this study was to conduct an integrative literature review on the regulation of L. monocytogenes in food in Brazil, its occurrence and methodologies used for identification in studies published from 2001 to 2021, and the epidemiological surveillance of cases of infection. The current regulations for L. monocytogenes prohibit ready-to-eat foods of more than 10 2 per gram or milliliter. Officially, only two cases of outbreaks have been identified in the Epidemiological Surveillance of Foodborne Diseases system; however, the circulation of L. monocytogenes was observed in foods of different origins, with occurrence ranging from 3.1 to 48.7. The most commonly used identification method was ISO 11290. The isolation and identification methods for this pathogen are expensive and laborious, making it difficult to implement these methodologies in many laboratories. This scenario contributes to the underreporting of cases of listeriosis and therefore represents a risk for the population that is exposed to potentially contaminated food.

show abstract

Section: Laboratories Of the National Health Surveillance Systemmentioning

confidence: 79%

Section: Identification Methodsmentioning

confidence: 99%

Listeria monocytogenes: challenges of microbiological control of food in Brazil

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Las pruebas serológicas como ELISA y la prueba rápida de seroaglutinación pueden detectar anticuerpos contra Salmonella spp. [3].…”

Section: Introductionunclassified

Re-emergencia de la Salmonella pullorum en ponedoras comerciales de Cochabamba, Bolivia

et al. 2022

View full text Add to dashboard Cite

En marzo de 2021, en el departamento de Cochabamba, provincia Quillacollo, Bolivia, se detectó un brote de Salmonelosis. Se trató de un lote de pollitas marrones de levante de la línea comercial H&N, edad 4 a 5 días.La mortalidad ascendió al 30 %, el resto mostraron todas signos de deshidratación, deposición de uratos en uréteres, hepatomegalia, taponamiento cloacal, esplenitis, saco vitelino de color verduzco, vesícula biliar licuefacta de color amarillo, molleja erosionada y ulcerosa. En cultivo bacteriológico de hígado, corazón y médula ósea se obtuvo exclusivamente Salmonella spp. (inmóvil) en regular cantidad, abundante cantidad en saco vitelino y escasa en bilis. La prueba de Acriflavina reveló presencia de cepa lisa (patógena). En algunas muestras crecieron además escasamente, Eschericha coli y Proteus spp. En incubadoras, los cultivos bacteriológicos de huevos picados o licuefactos mostraron crecimiento de Salmonella spp. Todas las muestras presentaron además alta contaminación con Proteus spp. y Pseudomonas spp. A las reproductoras de las pollitas infectadas les fue aplicado el fusil sanitario en la totalidad de los lotes. Los aislamientos bacteriológicos de hígado, bazo, médula ósea, tonsilas cecales e hisopados cloacales de otras reproductoras de la misma granja, resultaron negativos a Salmonella spp. Sin embargo, se aisló Salmonella spp. (inmóvil) de hisopados del contenido cloacal de aves reproductoras muertas. Las pruebas bioquímicas de Glucosa, Lisina descarboxilasa, Indol, Ornitina descarboxilasa y Manitol resultaron positivas con producción de ácido y gas. Se analizaron las muestras de ADN aisladas de varios casos positivos con PCR multiplex, confirmando la presencia de Salmonella enterica biovar Pullorum en un laboratorio oficial dependiente del Servicio Nacional de Sanidad Agropecuaria e Inocuidad Alimentaria “SENASAG”, Cochabamba, Bolivia, con lo cual queda oficialmente confirmado la presencia de Salmonella enterica biovar Pullorum.

show abstract

Development of a multiplex qPCR in real time for quantification and differential diagnosis ofSalmonellaGallinarum andSalmonellaPullorum

et al. 2017

View full text Add to dashboard Cite

Currently there are 2659 Salmonella serovars. The host-specific biovars Salmonella Pullorum and Salmonella Gallinarum cause systemic infections in food-producing and wild birds. Fast diagnosis is crucial to control the dissemination in avian environments. The present work describes the development of a multiplex qPCR in real time using a low-cost DNA dye (SYBr Green) to identify and quantify these biovars. Primers were chosen based on genomic regions of difference (RoD) and optimized to control dimers. Primers pSGP detect both host-specific biovars but not other serovars and pSG and pSP differentiate biovars. Three amplicons showed different melting temperatures (Tm), allowing differentiation. The pSGP amplicon (97 bp) showed Tm of 78°C for both biovars. The pSG amplicon (273 bp) showed a Tm of 86.2°C for S. Gallinarum and pSP amplicon (260 bp) dissociated at 84.8°C for S. Pullorum identification. The multiplex qPCR in real time showed high sensitivity and was capable of quantifying 10-10 CFU of these biovars.

show abstract

Métodos Diagnósticos Para Os Patógenos Alimentares: Campylobacter Sp., Salmonella Sp. E Listeria Monocytogenes

Cited by 9 publications

References 47 publications

Listeria monocytogenes: challenges of microbiological control of food in Brazil

Listeria monocytogenes: challenges of microbiological control of food in Brazil

Re-emergencia de la Salmonella pullorum en ponedoras comerciales de Cochabamba, Bolivia

Development of a multiplex qPCR in real time for quantification and differential diagnosis ofSalmonellaGallinarum andSalmonellaPullorum

Contact Info

Product

Resources

About