Fluoroquinolonas X Resistência Bacteriana Na Medicina Veterinária

Silva, J.M.B. da; Hollenbach, Clarissa Boemler

doi:10.1590/1808-1657v77p3632010

Cited by 12 publications

(6 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Ciprofloxacin was detected in the examined samples at variable rates. Such results were consistent with the findings of Silva and Hollenbach (2010 ), and Raphaella (2008 ), who showed that the kidneys and liver serve as the reservoir for ciprofloxacin residues. These findings were also supported by those published by Amjad et al .…”

Section: Discussionsupporting

confidence: 92%

Assessment of antibiotic residues in chicken meat

Kamouh,

Abdallah,

Kirrella

et al. 2024

Open Vet J

View full text Add to dashboard Cite

Background: Nowadays Veterinarians and poultry producers use antibiotics to increase growth rates, bird health, and feed efficiency, egg production, for preventative and therapeutic purposes, and to lessen the prevalence of poultry diseases. Most poultry producers have used a variety of antibiotics, either with or without veterinarian instruction. Although antibiotics are beneficial for the majority of their uses, their unauthorized use has resulted in residues accumulated in poultry products intended for human consumption which represent a serious risk to the general public that could be toxicological, microbiological or immunological. Aim: This study aimed at estimation of the residues of three major antimicrobial used in the intensive chicken rearing systems in Egypt, namely Oxytetracycline, Gentamicin, Ciprofloxacin. Moreover, the effect of cooking on such residues was investigated. Methods: A total of 100 chicken meat samples (breast, thigh, gizzard, liver, 25 each) were examined for detection of the aforementioned antimicrobials using the microbial inhibition assay and HPLC. Besides, samples containing the highest antimicrobial residues were examined for the effect of boiling for 30 min on such residues. Results: The obtained results revealed that 23%, 21%, and 17% of the examined samples were positive for oxytetracycline, gentamicin, and ciprofloxacin residues, respectively. As for cooking (boiling) for 30 minutes showed reduction of the antibiotic residue by 88.2%, 95.2%, and 31.3%, respectively. Conclusion: Antimicrobial residues were detected in the chicken meat parts retailed in Egypt. Cooking can reduce the antimicrobial residues at least in part.

show abstract

Section: Discussionsupporting

confidence: 92%

Assessment of antibiotic residues in chicken meat

Kamouh,

Abdallah,

Kirrella

et al. 2024

Open Vet J

View full text Add to dashboard Cite

show abstract

“…Fluoroquinolones have a high capacity to penetrate the cell wall of Gram‐positive and Gram‐negative bacteria, altering their biological activities, by inhibiting intracellular mechanisms. Thus, it is suggested that Meldrum acid derivatives could also act by reversing resistance mechanisms to fluoroquinolones at the intracellular level [30]. Such combinations can result in an increase in the antimicrobial spectrum, a reduction in its toxicity, the use of low doses of the drug, and the prevention of the selection of resistant bacteria during therapy [31].…”

Section: Discussionmentioning

confidence: 99%

Meldrum's acid derivates are MepA efflux pump inhibitors: In vitro and in silico essays

Araújo,

Pereira,

de Araújo

et al. 2023

J Basic Microbiol

View full text Add to dashboard Cite

Efflux pumps are proteins capable of expelling antibiotics from bacterial cells, have emerged as a major mechanism of bacterial resistance. In the ongoing pursuit to overcome and reduce bacterial resistance, novel substances are being explored as potential efflux pump inhibitors. Meldrum's acid, a synthetic molecule widely studied for its role in synthesizing bioactive compounds, holds promise in this regard. Therefore, the objective of this study is to evaluate the antibacterial activity of three derivatives of Meldrum's acid and assess their ability to inhibit efflux mechanisms, employing both in silico and in vitro approaches. The antibacterial activity of the derivatives was assessed using a broth microdilution testing method. Surprisingly, the derivatives did not exhibit direct antibacterial activity on their own. However, they displayed a significant effect in enhancing the efficacy of antibiotics, suggesting a potential role in potentiating their effects. Furthermore, fluorescence emission assays using ethidium bromide indicated that the derivatives could potentially block efflux pumps, as they exhibited fluorescence levels comparable to the positive control. To further investigate their inhibitory capacity, molecular docking studies were conducted in silico, revealing binding interactions similar to ciprofloxacin and carbonyl cyanide 3‐chlorophenylhydrazone, known efflux pump inhibitors. These findings highlight the potential of Meldrum's acid derivatives as effective inhibitors of efflux pumps. By targeting these mechanisms, the derivatives offer a promising avenue to enhance the effectiveness of antibiotics and combat bacterial resistance. This study underscores the importance of exploring novel strategies in the fight against bacterial resistance and provides valuable insights into the potential of Meldrum's acid derivatives as efflux pump inhibitors. Further research and exploration in this field are warranted to fully exploit their therapeutic potential.

show abstract

“…As fluorquinolonas atualmente são uma das maiores classes de agentes antimicrobianos sendo utilizadas, mundialmente, no tratamento de infecções de origem bacteriana (Martinez et al, 2006;Trouchon & Lefebvre, 2016). Este grupo de fármacos foi se desenvolvendo de forma a ultrapassar grande parte das suas adversidades, possuindo um espectro de ação amplo, boa disponibilidade oral, melhor difusão para os tecidos, uma meia vida maior e uma toxicidade consideravelmente reduzida (Silva & Hollenbach, 2010;Trouchon & Lefebvre, 2016). De acordo com Scholar (2002) as fluoroquinolonas atuam por inibição da atividade catalítica de duas enzimas responsáveis e essenciais à replicação e transcrição do DNA bacteriano: a DNA girase e a topoisomerase IV (topoisomerases do tipo II).…”

Section: Introductionunclassified

“…De acordo com Scholar (2002) as fluoroquinolonas atuam por inibição da atividade catalítica de duas enzimas responsáveis e essenciais à replicação e transcrição do DNA bacteriano: a DNA girase e a topoisomerase IV (topoisomerases do tipo II). A enrofloxacina tem uma substituição de flúor, a difloxacina tem duas substituições de flúor e a orbifloxacina tem uma substituição de três moléculas de flúor, mas a presença de mais de um flúor não aumenta os efeitos antibacterianos (Silva & Hollenbach, 2010;Trouchon & Lefebvre, 2016). As fluoroquinolonas são considerados antibióticos lipossolúveis, porem dentre deste grupo os menos lipossolúveis como marbofloxacina, orbifloxacina têm um volume de distribuição (Vd) menor do que aquelas com maior lipossolubilidade como enrofloxacina e difloxacina (Frazier et al, 2000).…”

Section: Introductionunclassified

Comparação da eficácia de difloxacina e orbifloxacina no tratamento de infeções de E. Coli e S. Intermedius em cães

Paiva¹,

Silva²,

Canassa³

et al. 2018

Pubvet

View full text Add to dashboard Cite

O objetivo do presente trabalho foi avaliar e comparar o efeito de mudanças na CIM, nas taxas de eficácia de difloxacina e orbifloxacina no tratamento das infecções bacterianas causadas por Escherichia coli (E. coli) ou Staphylococcus intermedius (S. intermedius) em cães. Realizou-se o modelamento farmacocinético/farmacodinâmico (PK/PD) para a determinação da eficácia do tratamento com 5 mg/kg de diclofaxina ou 2,5mg/kg de orbifloxacina via oral a cada 24 horas de infeções causadas por E. coli S. intermedius em cães. Foi realizada uma simulação de Monte Carlo dos parâmetros farmacocinéticos de 10.000 eventos. Os valores de PK foram obtidos de estudos realizados em cães, onde determinaram valores de ASC24 de 9.34 ± 2.09 ug/ml para difloxacina e de

show abstract

Fluoroquinolonas X Resistência Bacteriana Na Medicina Veterinária

Cited by 12 publications

References 18 publications

Assessment of antibiotic residues in chicken meat

Assessment of antibiotic residues in chicken meat

Meldrum's acid derivates are MepA efflux pump inhibitors: In vitro and in silico essays

Comparação da eficácia de difloxacina e orbifloxacina no tratamento de infeções de E. Coli e S. Intermedius em cães

Contact Info

Product

Resources

About