Estudo Comparativo Entre Os Testes Adotados Pelo Pncebt Para O Diagnóstico Sorológico Da Brucelose Em Bovinos

Meirelles-Bartoli, R. B.; Mathias, Luís Antônio

doi:10.1590/1808-1657v77p0112010

Cited by 9 publications

(1 citation statement)

References 13 publications

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“…The occurrence of false-negative results is usually due to prozone phenomena, which, in turn, can be reduced by serum dilution or retesting after some time or by performing a more specific and confirmatory test such as the FC ( Organização Mundial da Saúde Animal, 2018 ). In a study conducted by Meirelles-Bartoli and Mathias (2010) , the authors reported relative sensitivity values of 99.6%, 98.8%, and 91.1%, respectively, for AAT, 2-ME, and FC. The comparison between the three tests adopted by the program highlighted a good agreement between the Acidified Buffered Antigen (AAT) and the confirmatory tests (2-ME, with Kappa = 0.80; and FC, with Kappa= 0.73).…”

Section: Discussionmentioning

confidence: 99%

Bovine brucellosis seroprevalence and flow network analysis in slaughterhouses in the state of Ceará

et al. 2022

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The present study aimed to assess the prevalence and risk factors associated with bovine brucellosis in slaughterhouses in the state of Ceará using spatial distribution and flow network analysis. Four slaughterhouses were sampled in Ceará: two under municipal inspection and two under state inspection. Blood samples were randomly collected from bovine animals, resulting in a total of 964 samples. The collected sera were subjected to the Acidified Buffered Antigen (AAT) test, and the complement fixation test (FC) was performed for positive cases. An epidemiological questionnaire was applied to 38 producers who slaughter animals at the sampled facilities to assess the risk factors for brucellosis. An apparent prevalence of 1.55% (15) was found in the AAT test and 0.2% (n=2) in the FC test. A higher percentage of reactive animals was observed (66.6%) in properties where cattle farming is not the main activity, with OR = 4.75. The absence of contact with neighboring animals is a factor associated with protection, with a lower prevalence of seroreactive animals (23.5%) when animals were raised without contact with others (OR = 0.30). Therefore, bovine brucellosis in herds and animals can be considered low in the studied region and under all production systems. Nevertheless, despite the importance of this disease to the economic and public health aspects and the advances of the PNCEBT Program, brucellosis is still circulating in Ceará.

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Section: Discussionmentioning

confidence: 99%

Bovine brucellosis seroprevalence and flow network analysis in slaughterhouses in the state of Ceará

et al. 2022

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Evaluation of dot-blot test for serological diagnosis of bovine brucellosis

Bastos

Mathias

Jusi

et al. 2018

Brazilian Journal of Microbiology

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The objective of this study was to standardize and validate the dot-blot test for the serological diagnosis of bovine brucellosis, compare the results with those found in the 2-mercaptoethanol (2-ME) and complement fixation test (CF), and estimate the relative sensitivity and specificity of the dot-blot compared to these tests. Fifty bovine blood serum samples were used for the test standardization, and 1315 samples were used for evaluation and comparison between the tests; the results were compared using the Kappa indicator. At the end of standardization, it was established as optimal for the antigen obtained from Brucella abortus B19 after passing through a microorganism rupture process, the blood serum samples diluted at 1:100, and the conjugate at 1:30,000. The comparison of the dot-blot results with 2-ME showed Kappa index of 0.9939, sensitivity of 99.48%, and specificity 99.91%, with CF, Kappa index of 0.8226, sensitivity 100% and specificity 95.32%. Using the combination of the test results 2-ME and CF to establish the true condition of the animal, the dot-blot showed relative sensitivity of 100%, and relative specificity of 99.91%. The evaluated test proved to be effective and reliable, besides being easy to handle and interpret the results.

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Turning chaotic sample group clusterization into organized ones by feature selection: Application on photodiagnosis of Brucella abortus serological test

de Rezende,

Franca,

de Paula

et al. 2023

Journal of Photochemistry and Photobiology B: Biology

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Estudo Comparativo Entre Os Testes Adotados Pelo Pncebt Para O Diagnóstico Sorológico Da Brucelose Em Bovinos

Cited by 9 publications

References 13 publications

Bovine brucellosis seroprevalence and flow network analysis in slaughterhouses in the state of Ceará

Bovine brucellosis seroprevalence and flow network analysis in slaughterhouses in the state of Ceará

Evaluation of dot-blot test for serological diagnosis of bovine brucellosis

Turning chaotic sample group clusterization into organized ones by feature selection: Application on photodiagnosis of Brucella abortus serological test

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