Patogenicidade De Nematóides Entomopatogênicos a Cochonilha-Daraiz-Do-Cafeeiro Dysmicoccus Texensis (Tinsley) (Hemiptera: Pseudococcidae) Em Laboratório

Alves, Viviane Sandra; Moino, Alcides; Santa-Cecília, Lenira Viana Costa; Andaló, Vanessa; Souza, Giselle C.

doi:10.1590/1808-1657v76p0672009

Cited by 10 publications

(1 citation statement)

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“…Such differences in the virulence of different isolates, even those of the same species, can be explained by several factors. Different isolates may have adapted to different climatic conditions or even developed local host specificities (Alves et al, 2009). According to Doucet et al (1999), a variation in susceptibility is to be expected, given that different isolates have co-evolved with different host species, and therefore have specificities that render them more or less virulent to certain insects, which may explain the differences observed in the present study.…”

Section: Andaló Et Al (2009b)mentioning

confidence: 72%

First report and biological characteristics of Heterorhabditis amazonensis in the state of Paraná, Brazil

et al. 2024

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Entomopathogenic nematodes (EPNs) from Heterorhabditidae and Steinernematidae families are extensively used to control insect pests. In Brazil, however, relatively few studies have identified and characterized these entomopathogens. The objective of this study was to identify and characterize an EPN isolate obtained from soil samples collected in the state of Paraná, Brazil. An isolate (UEL 08) of Heterorhabditis was detected in a soil sample collected from a pasture area cultivated with Brachiaria grass in Londrina, state of Paraná, Brazil (23°34ʹ311ʹʹS, 050°58ʹ298ʹʹW), using the insect-baiting technique with Galleria mellonella larvae as hosts. The nematode was identified through morphometric studies and molecular analyses based on amplification of the rDNA ITS region. Although we identified certain morphometric differences compared with the original description, the molecular data indicated that the ITS sequence obtained for the UEL 08 isolate is identical to the reference sequence of H. amazonensis (DQ665222) and presented 100% similarity. Thus, the findings of our morphological and molecular studies confirmed that the isolated nematode is H. amazonensis, which is the first time this species has been registered in Paraná. Study of the biological characteristics of H. amazonensis (UEL 08) revealed that the isolate has two distinct life cycles – one short (216 h) and the other long (288 h) – and produces two generations in both cycles. We observed that H. amazonensis (UEL 8) was pathogenic and virulent to the three evaluated hosts, although with different virulence against these hosts. The larvae of G. mellonella and Alphitobius diaperinus were more susceptible than adult Dichelops (Diacereus) melacanthus, with 100%, 85%, and 46% mortality, respectively. Furthermore, an in vivo production assay revealed a mean daily yield of 3.4 × 103 infective juveniles/g host larvae.

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Section: Andaló Et Al (2009b)mentioning

confidence: 72%