Efficacy of parasitological diagnosis methods in wild animals kept in captivity

Abstract

Parasitic diseases are common in wild animals kept in captivity, and the research of these parasites is a tool used in wildlife management actions. Once the method most used in the practice of veterinary laboratories is the direct examination, it was considered appropriate to report the parasitism in captivity wildlife comparing this technique with modified methods of Hoffman, Pons and Janer (HPJ) and Willis. Fresh feces were surveyed in eleven precincts with specimens of the class Reptilia, Birds and Mammalia. A positive result for the presence of parasites was observed in 81.8% of them, of which 18.2% were, 9.1% helminths and 54.6% protozoa and helminths simultaneously. The direct and HPJ methods were more specific for the identification of parasites, 72.7 and 63.6%, respectively, while the method of Willis showed less positive results (36.4%). It is concluded that the ideal routine in veterinary laboratory fecal examinations of wild animals is to use the HPJ method associated with the direct method for the detection of protozoa and/or helminths, in order to obtain more accurate results.

Evaluation of General Health Status of Some Aged Tigresses at a Local Circus, Cairo, Egypt

“…Following the guidelines of (Carvalho et al, 2018 andDe Carli 2001), samples of fresh feces were taken from the collected specimens. First, they were diluted with 1mL buffered saline solution between the slide and cover slip and examined by the direct method of observation under the microscope.…”