Standardization and validation of an indirect ELISA based on the extraction of proteins from Leptospira spp. as an antigen for the diagnosis of canine leptospirosis
Thaise Alves,
Bruno Fernandes,
Vinícius Vieira
et al.
Abstract:Leptospirosis is an important zoonosis. The World Health Organization recommends the use of microscopic soroagglutination in the diagnosis of the disease. However, this is a time-consuming technique that offers a risk of contamination to the performer and requires a trained professional. The indirect enzyme-linked immunosorbent assay (ELISA) test presents less risk, is faster, and has lower cost benefit for high sampling. In view of the advantages of using the test, and the importance of the development of tec… Show more
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