Bioactive glass-ceramic for bone tissue engineering: an in vitro and in vivo study focusing on osteoclasts

Bighetti‐Trevisan, Rayana Longo; Souza, Alann Thaffarell Portilho; Tosin, Ingrid Wezel; Bueno, Natália Pieretti; Crovace, Murilo C.; Beloti, Márcio Mateus; Rosa, Adalberto Luiz; Ferraz, Emanuela Prado

doi:10.1590/1807-3107bor-2022.vol36.0022

Cited by 4 publications

(2 citation statements)

References 39 publications

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“…The functional effects of the biomaterials used for bone grafting on osteoclast activity remain controversial. Scaffolds containing silica-based glass can accelerate the viability and osteoclast differentiation of RAW 264.7, promoting bone regeneration when compared with control scaffolds (Bighetti-Trevisan et al 2022). Conversely, strontium-incorporated silica-based glass scaffolds can enhance bone regeneration by inhibiting osteoclast activity (Wei et al 2014).…”

Section: Discussionmentioning

confidence: 99%

Zinc- and Fluoride-Releasing Bioactive Glass as a Novel Bone Substitute

Kondo,

Otake,

Kakinuma

et al. 2024

J Dent Res

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Bioglass 45S5, a silica-based glass, has pioneered a new field of biomaterials. Bioglass 45S5 promotes mineralization through calcium ion release and is widely used in the dental field, including toothpaste formulations. However, the use of Bioglass 45S5 for bone grafting is limited owing to the induction of inflammation, as well as reduced degradation and ion release. Phosphate-based glasses exhibit higher solubility and ion release than silica-based glass. Given that these glasses can be synthesized at low temperatures (approximately 1,000°C), they can easily be doped with various metal oxides to confer therapeutic properties. Herein, we fabricated zinc- and fluoride-doped phosphate-based glass (multicomponent phosphate [MP] bioactive glass) and further doped aluminum oxide into the MP glass (4% Al-MP glass) to overcome the striking solubility of phosphate-based glass. Increased amounts of zinc and fluoride ions were detected in water containing the MP glass. Doping of aluminum oxide into the MP glass suppressed the striking dissolution in water, with 4% Al-MP glass exhibiting the highest stability in water. Compared with Bioglass 45S5, 4% Al-MP glass in water had a notably reduced particle size, supporting the abundant ion release of 4% Al-MP glass. Compared with Bioglass 45S5, 4% Al-MP glass enhanced the osteogenesis of mouse bone marrow–derived mesenchymal stem cells. Mouse macrophages cultured with 4% Al-MP glass displayed enhanced induction of anti-inflammatory M2 macrophages and reduced proinflammatory M1 macrophages, indicating M2 polarization. Upon implanting 4% Al-MP glass or Bioglass 45S5 in a mouse calvarial defect, 4% Al-MP glass promoted significant bone regeneration when compared with Bioglass 45S5. Hence, we successfully fabricated zinc- and fluoride-releasing bioactive glasses with improved osteogenic and anti-inflammatory properties, which could serve as a promising biomaterial for bone regeneration.

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Section: Discussionmentioning

confidence: 99%

Zinc- and Fluoride-Releasing Bioactive Glass as a Novel Bone Substitute

Kondo,

Otake,

Kakinuma

et al. 2024

J Dent Res

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“…Além disso, apesar de ambas as culturas não proliferarem a partir do sétimo dia, com exceção do terceiro dia o número de DM-MSC foi menor que NG-MSC nos demais períodos de proliferação celular avaliados, semelhante ao observado anteriormente (BUENO et al, 2022;BUENO et al, 2021). Dessa forma, como as MSC contribuem para a manutenção da homeostase óssea sendo fonte de progenitores para os osteoblastos, agentes anabólicos e reguladores da osteoclastogênese (KFOURY;SCADDEN, 2018), os resultados sugerem que ao menos em parte, a perda óssea na DM pode estar relacionada à redução da capacidade de diferenciação osteoblástica de MSC, semelhante ao observado em outros estudos (GHODSI et al, 2015).…”

Section: Análise Histológica Do Tecido óSseo Formado Em Defeitos Cria...unclassified

Efeitos das interações entre células-tronco mesenquimais de ratos saudáveis e com osteoporose, diabetes mellitus ou hipertensão arterial na diferenciação osteoblástica e na regeneração de defeitos ósseos

Souza¹

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as ORX-MSC não prejudicaram a diferenciação de SD-MSC, as DM-MSC prejudicam pouco e as HA-MSC prejudicam ainda mais essa diferenciação. Nessas interações houve modulação de genes das vias de sinalização das BMP, WNT e ITG em diferentes graus. As SD-MSC permanecerem nos defeitos de todas as doenças e a terapia celular resultou em maior formação óssea comparada à injeção de PBS nos defeitos de animais com osteoporose e HA, mas não com DM. Os resultados desse estudo são relevantes para o desenvolvimento de terapia celular com vistas à regeneração do tecido ósseo em condições adversas como a osteoporose, DM ou HA.

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