Protective effect of green tea catechins on eroded human dentin: an in vitro/in situ study

Moraes, Maria Denise Rodrigues de; Passos, Vanara Florêncio; Padovani, G; Bezerra, Lady Clarissa Brito da Rocha; Vasconcelos, Ilka M.; Santiago, Sérgio Lima

doi:10.1590/1807-3107bor-2021.vol35.0108

Cited by 5 publications

(7 citation statements)

References 41 publications

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“…EGCG has been identi ed as an inhibitor of MMPs 2 and 9, with minimum inhibitory concentration values of 6 and 0.8 µM, respectively [43]. Therefore, the concentration of 0.1% or 4000 µM used in the current study was considered su cient to inhibit these collagenases, as showed in a previous study [31]. However, in the present study, the wear was like EGCG gel and negative control.…”

Section: Discussionmentioning

confidence: 58%

“…Five indentations 100 µm apart were made in the center of the specimens using a microhardness tester with Knoop indenter and automatic measuring system (Future Tech Corp, FM-ARS 9000 and FM 100, Tokyo, Japan). A Knoop diamond indenter was used with a load of 10 g applied for 5s [21,31]. Eighty-eight dentin specimens, which had an average hardness of 54.53 ± 5.4 Kg/mm², were selected, packaged, and identi ed for ethylene oxide sterilization.…”

Section: Preparation Of Dentin Specimensmentioning

confidence: 99%

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Gel Containing Catechin and Mesoporous Silica Nanoparticles for Protecting Root Dentin Against Erosion: An In Situ Study

Alves,

Gomes,

Uchoa

et al. 2024

Preprint

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Objective This study aimed to evaluate the in situ anti-erosive effect of gels containing epigallocatechin-3-gallate (EGCG) isolated and adsorbed on mesoporous silica nanoparticles (EGCG/MSN) on eroded dentin. Materials and Methods Eleven volunteers participated in this randomized, controlled, cross-over study, which consisted of 4 phases of 5 days. The participants wore an acrylic palatal device containing two dentin blocks treated with one of the gels: placebo (negative control), SnF2 (0.05% - positive control), EGCG (0.1%), and EGCG/MSN (0.093%). During each phase, the specimens were immersed in citric acid (0.05 M; pH 3.75) for 60 s, 4x/day, followed by treatment with the assigned gel for 60 s. The alterations were evaluated by measuring the percentage of surface hardness loss (%SHL) and through profilometry analysis (wear). Morphological changes were assessed using scanning electron microscopy (SEM). The data were analyzed using ANOVA, followed by Tukey's post-test. Results %SHL did not show a significant difference among the groups (p = 0.067). Regarding surface wear, the mean results in micrometers were: placebo, 0.66 (± 0.38); EGCG, 0.57 (± 0.11); EGCG/MSN, 0.48 (± 0.05); and SnF2, 0.32 (± 0.08). A significant difference was observed only between the SnF2 group and the placebo and EGCG groups (p = 0.003 and p = 0.046, respectively). However, there was no difference between the SnF2 and EGCG/MSN groups (p = 0.306). Conclusion EGCG/MSN shows promise as a protective measure in reducing dentin wear under erosive conditions. Clinical Relevance: Gels containing EGCG adsorbed on mesoporous silica nanoparticles have a protective effect against dentin erosion.

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Section: Discussionmentioning

confidence: 58%

Section: Preparation Of Dentin Specimensmentioning

confidence: 99%

Gel Containing Catechin and Mesoporous Silica Nanoparticles for Protecting Root Dentin Against Erosion: An In Situ Study

Alves,

Gomes,

Uchoa

et al. 2024

Preprint

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“…In the terms of flavonoids and flavonoid glycosides, GTE mainly consisted of kaempferol‐3‐ O ‐galactoside (Kae‐3‐ O ‐Gal, 10.23 ± 0.97 mg/g) and myricetin‐3‐ O ‐glucoside (Myr‐3‐ O ‐Glu, 7.80 ± 0.70 mg/g) (Figure 1d). Previous studies have provided evidence that catechins, AA, and CAF decreased inflammatory response in oral epithelial cells, alleviated oral submucous fibrosis, and protected teeth against acid‐induced erosion (De Moraes et al., 2021; Vaillancourt et al., 2021). Moreover, research has shown that oral administration of flavonoids like diosmin and hesperidin inhibited leukocytes adhering to the venular endothelium in the hamster cheek (Bouskela & Donyo, 1997).…”

Section: Resultsmentioning

confidence: 99%

“…In vitro, GTE has demonstrated the ability to inhibit the growth of cariogenic and periodontopathic bacteria, including S. mutans, A. actinomycetemcomitans, Porphyromonas gingivalis, and Prevotella intermedia (Araghizadeh et al, 2013;Kong et al, 2022). Furthermore, GTE has exhibited promising effects in alleviating oral diseases, such as oral cancer, dental erosion, and halitosis (De Moraes et al, 2021;Liao et al, 2021;Sharma et al, 2023). In terms of modulating probiotics, Zhang et al (2013) found that GTE significantly increased the abundance of beneficial bacteria such as Bifidobacterium in vitro.…”

Section: Introductionmentioning

confidence: 99%

Green tea extracts alleviate acetic acid‐induced oral inflammation and reconstruct oral microbial balance in mice

Pan,

Lv,

Zhang

et al. 2023

Journal of Food Science

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Oral cavity contains the second largest microbial community in the human body. Due to the highly vascularized feature of mouth, oral microbes could directly access the bloodstream and affect the host healthy systemically. The imbalance of oral microbiota is closely related to various oral and systemic diseases. Green tea extracts (GTE) mainly contain tea polyphenols, alkaloids, amino acid, flavones, and so on, which equipped with excellent anti‐inflammatory activities. Previous studies have demonstrated the beneficial effects of GTE on oral health. However, most researches used in vitro models or focused on limited microorganisms. In this study, the regulatory effect of GTE on oral microbiome and the alleviative effect on oral inflammation in vivo were evaluated. The results showed that GTE could efficiently alleviate the inflammations of the tongue, cheek pouch, as well as throat. GTE effectively inhibited the activation of NF‐κB through the upregulation of the anti‐inflammatory cytokine interleukin (IL)‐10, consequently leading to reduced expression of pro‐inflammatory cytokines IL‐6 and tumor necrosis factor‐α. The indexes of spleen and thymus were also elevated by GTE in stomatitis mice. Moreover, GTE promoted the growth of probiotics Lactobacillus and Bacillus, inhibited the reproduction of pathogens Achromobacter, reversing the microbiota disorders in oral cavity. This study not only presents a novel approach for enhancing oral microecology but also facilitates the wider adoption of tea consumption.

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“…10,11 EGCG has been demonstrated to prevent or treat oral diseases by protecting the oral mucosa and decreasing enamel and dentin erosion. [12][13][14] The inhibitory effects of EGCG on oral pathogens have been demonstrated. For instance, EGCG has been proven to effectively restrain the expression of the gtfB, gtfC, and gtfD genes, which are related to bacterial biofilm formation, thereby suppressing the initial attachment of pathogens.…”

Section: Introductionmentioning

confidence: 99%

Epigallocatechin gallate (EGCG) alleviates the inflammatory response and recovers oral microbiota in acetic acid-induced oral inflammation mice

Pan,

Lv,

Feng

et al. 2023

Food Funct.

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Protective effect of green tea catechins on eroded human dentin: an in vitro/in situ study

Cited by 5 publications

References 41 publications

Gel Containing Catechin and Mesoporous Silica Nanoparticles for Protecting Root Dentin Against Erosion: An In Situ Study

Gel Containing Catechin and Mesoporous Silica Nanoparticles for Protecting Root Dentin Against Erosion: An In Situ Study

Green tea extracts alleviate acetic acid‐induced oral inflammation and reconstruct oral microbial balance in mice

Epigallocatechin gallate (EGCG) alleviates the inflammatory response and recovers oral microbiota in acetic acid-induced oral inflammation mice

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