Involvement of tryptase-positive mast cells and angiogenesis in the growth of inflammatory odontogenic cysts

Abstract

Inflammatory periapical lesions are characterized by infiltration of different immune cell types, the functions of which depend on an effective vascular network. This study aimed to evaluate the mast cells density (MCD) in inflamatory odontogenic cysts capsules concerning microvascular density (MVD), microvascular area (MVA), and microvascular perimeter (MVP), and correlate such findings with the type of lesion, intensity of the inflammatory infiltrate, and thickness of the epithelial lining. Twenty inflamatory dentigerous cysts (IDCs), twenty radicular cysts (RCs), and twenty residual radicular cysts (RRCs) were submitted to immunohistochemical analysis using anti-tryptase and anti-CD34 antibodies. RCs exhibited the highest MCD, MVD, MVA, and MVP indexes (p = < 0.001, p = 0.008, p = 0.003 and p = < 0.001, respectively), and lesions with inflammatory infiltrate grade III showed the highest MVD (p = 0.044). Considering epithelial thickness, a higher MVP index was identified in lesions with hyperplastic epithelium (p = 0.018). In IDCs, RCs, and RRCs, a strong positive correlation was observed between MVA and MVP (r = 0.950 and p = < 0.001; r = 0.914 and p = < 0.001; r = 0.713 and p = < 0.001, respectively). In IDCs, a moderate correlation was observed between MCD and both MVA and MVP (r = 0.660 and p = 0.002; r = 0.634 and p = 0.003, respectively). These results suggest that tryptase-positive mast cells might play an important role in the angiogenic activity of IDCs, while RCs had the highest indexes. Our findings also confirmed that the intensity of the inflammatory infiltrate and epithelial thickness influence angiogenesis.

Impact of CD34-dependent Micro Vessel Density on Periapical Odontogenic Cysts

“…In the current study, we estimated MVD levels in a fast and accurate way using a digitized algorithm based on CD34 IHC expression levels. Our previous studies on oral malignancies are concordant with similar experimental studies (29)(30)(31)(32)(33)(34)(35). All of them suggest and enhance this practice because it provides a systematic screening and mapping of immunostained slides.…”

Mast Cell Tryptase and Carboxypeptidase A3 in the Formation of Ovarian Endometrioid Cysts

“…In the context of the endometrioid cyst pathogenesis, data on the close tryptase involvement in angiogenesis processes are of great interest [ 24 , 62 , 63 ]. Tryptase is a part of several mechanisms of new blood vessel growth and differentiation, including inflammation and tumorigenesis [ 64 , 65 , 66 ]. Concurrently, the formation of new vessels is combined with a pronounced remodeling of the connective tissue and the degradation of the amorphous and fibrous components of the extracellular matrix, due to the tryptase-induced activation of matrix metalloproteinases (MMPs), in particular, MMP-1, MMP-2, MMP-3, MMP-9, and MMP-13, etc.…”