Can kinematics, file diameter, and PUI influence the intracanal decontamination and apical bacterial extrusion?

Cuéllar, M. R. C.; Velásquez-Espedilla, Evelyn Giuliana; Pedrinha, Victor Feliz; Vivan, Rodrigo Ricci; Duarte, Marco Antônio Húngaro; Andrade, Flaviana Bombarda de

doi:10.1590/1807-3107bor-2021.vol35.0003

Cited by 5 publications

(2 citation statements)

References 35 publications

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“…Absorbent paper point #20 (Dentsply Maillefer, Ballaigues, Switzerland) was inserted into the canals for 1 min each and then transferred to microtubes containing 1 mL of BHI broth according to previous studies. 10,26 The microtubes were vortexed for 10 s, and 100 μL of the content was transferred by dilution to other microtubes with 900 µL of BHI until a concentration of 10 -3 was reached. Next, 100-μL aliquots of the dilutions were plated on Petri dishes with BHI agar, which were stored in a bacteriological incubator at 37°C for 48 h for subsequent CFU/ mL counts.…”

Section: Microbiological Samplesmentioning

confidence: 99%

“…The apical third of the tooth was not used due to the reduced number of dentinal tubules and variable anatomy, as previously observed. 21,24,25,26 In total, 80 images were obtained for each group, as well as 48 images for the positive controls (C + ) and 16 for the negative controls (C -). The images were obtained at a 1-μm step size deep and a resolution of 1024x1024 pixels.…”

Section: Preparation For Confocal Laser Scanning Microscopy (Clsm) Analysismentioning

confidence: 99%

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Impact of irrigation protocols with some chelators and mechanical agitation on intratubular decontamination

et al. 2021

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This study evaluated main canal and intratubular decontamination using different irrigation solutions followed by adjunctive agitation steps for infected root canals. Sixty-eight lower incisors were contaminated with Enterococcus faecalis and allocated to groups according to canal treatment (n = 10): G1, NaOCl followed by ethylenediaminetetraacetic acid (EDTA); G2, a mixture of NaOCl with hydroxyethylidene bisphosphonate (HEBP); and G3, NaOCl followed by EDTA-T (EDTA with sodium lauryl ether sulfate). All three groups of teeth were agitated with passive ultrasonic irrigation (PUI) using saline solution, whereby G4, G5, and G6 were prepared as above, and agitation was performed using an XP-Endo Finisher instrument. Microbiological samples were collected from the root canals with paper points at three times: before and after chemomechanical preparation and after agitation. The colony-forming units (CFU)/mL count was determined, and bacterial intratubular viability was analyzed via confocal laser scanning microscopy using Live/Dead staining. Statistical analysis was performed using a Kruskal-Wallis test followed by Dunn tests. A Friedman test was applied for colony-counting data (α = 0.05). CFU/mL counting indicated equally effective decontamination in the experimental groups (p > 0.05). According to microscopy images, the use of irrigation solutions followed by agitation with the XP-Endo Finisher yielded better results. Moreover, NaOCl+EDTA-T followed by XP-Endo Finisher resulted in significantly lower viability than in the PUI-activated groups (p < 0.05). The cervical and medium thirds of the specimens presented similar results. Overall, NaOCl+EDTA-T exhibited the best intratubular antibacterial activity, mainly for canals that were subsequently agitated using XP-Endo Finisher.

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Section: Microbiological Samplesmentioning

confidence: 99%

Section: Preparation For Confocal Laser Scanning Microscopy (Clsm) Analysismentioning

confidence: 99%

Impact of irrigation protocols with some chelators and mechanical agitation on intratubular decontamination

et al. 2021

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Influence of pecking frequency at working length on the volume of apically extruded debris: A micro-computed tomography analysis

Wang

Chen

Lin

et al. 2022

Journal of Dental Sciences

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Evaluation of irrigant extrusion following the use of different root canal irrigation techniques: A systematic review and meta‐analysis

Mazreah¹,

Shirvani

Mazreah³

et al. 2022

Aust Endodontic J

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This systematic review investigates whether different irrigation techniques have different effects on irrigant extrusion from mature tooth apices. Articles published between January 2000 and January 2022 were searched in six electronic databases (MEDLINE, Embase, Google Scholar, Web of Science, Scopus and Cochrane) using appropriate keywords. Overall, 2265 articles were screened by their titles and abstracts. Fifty‐six full‐text articles were selected based on the inclusion criteria. Of them, 17 in vitro studies were included in the systematic review and meta‐analysis. The meta‐analysis was conducted using the random‐effects inverse variance method. The results showed that the negative pressure technique caused a lesser amount (p = 0.00) and frequency (p = 0.00) of extrusion than the open‐ended needle irrigation. Sonic and ultrasonic activation caused less amount of extrusion than both open‐ended (p = 0.00 or p = 0.01) and closed‐ended needle (p = 0.00) irrigation.

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Can kinematics, file diameter, and PUI influence the intracanal decontamination and apical bacterial extrusion?

Cited by 5 publications

References 35 publications

Impact of irrigation protocols with some chelators and mechanical agitation on intratubular decontamination

Impact of irrigation protocols with some chelators and mechanical agitation on intratubular decontamination

Influence of pecking frequency at working length on the volume of apically extruded debris: A micro-computed tomography analysis

Evaluation of irrigant extrusion following the use of different root canal irrigation techniques: A systematic review and meta‐analysis

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