Effects of restraint stress and high-fat diet on osseointegration of titanium implants: an experimental study

Abstract

This study aimed to investigate the effects of chronic restraint stress (RS) and a high-fat diet (HFD) on the osseointegration of titanium implants in a rat model. After the surgical insertion of titanium implants into the metaphysis of the tibial bone, the rats were randomly divided into four equal groups (n = 8 each): control (CNT), restraint stress (RS), high-fat diet (HFD), and restraint stress plus high fat diet (RS-HFD). CNT: Rats received no further treatment during the 92-day experimental period. RS: Stress was applied to the rats beginning from two days after the implant surgery for one hour per day for the first 30 days, two hours per day for the next 30 days, and three hours per day for the last 30 days. HFD: Rats were fed a HFD for the following 90 days starting two days after surgery. RS-HFD: Rats were fed a HFD and RS was applied to rats for the following 90 days, starting two days after surgery. At the end of the experimental period, the rats were euthanized, and the implants and surrounding bone tissues were removed for histological analysis. Statistical analysis was performed by one way ANOVA and Bonferrroni tests. There were no significant differences in the bone-implant connection levels between the groups (p > 0.05), but in the HFD and RS-HFD groups, the bone filling ratios were found to be lower compared with the controls (p < 0.05) The data analyzed in this study suggest that an HFD with or without chronic RS adversely affected bone tissue in the rats during the 90-day osseointegration period.

The effects of dual anti-platelet therapy on titanium implant osseointegration: an experimental study

“…During the experiment, the rats were not limited in terms of food and water, and the light cycle in the lab was adjusted to rotate through 12 hours of darkness and 12 hours of brightness. All of the rats were selected in the same estrus period to ensure standardization throughout the experimental protocols (13).…”

“…Many factors are known to affect wound and bone healing. As for osseointegration, defined as the integration of bones and implants, the main factors impacting its success are the quality and amount of bone placed in the dental implant;, the patient's systemic diseases, smoking and drug use or lack thereof; and the geometrical and surface features of the implant device (12,13). Osseointegration is critical for a dental implant's success.…”

“…Infections, loss of function, and pain are a few of the problems that could lead to the failure of the implant during the bone healing process. On the other hand, some circumstances and applications that can positively affect this process may contribute to more successful dental implant outcomes (12,13).…”

Effects of Systemic Propranolol Application on the New Bone Formation in Periimplant Guided Bone Regeneration

“…In addition, implants are applied to various bones in the body (such as mandiblemaxilla). Due to the application of the experiment to the tibia, the results may be different in implants applied to different regions [36].…”

Потенційний Вплив Особливостей Дієти На Процес Остеоінтеграції Дентальних Імплантатів

“…Супровідно зареєстрований значимо вищий рівень тригліцеридів у дослідній групі міг також свідчити про те, що механізм впливу перенасиченої жирами дієти на процес остеоінтеграції є комплексним та реалізується за рахунок декількох шляхів, включаючи метаболізм жиру в крові. У ще одному дослідженні Dundar et al (2020) не вдалось виявити статистично доведеного негативного впливу дієти із високим вмістом жирів не тільки на площу контакту кісткової тканини та поверхні імплантатів, але й на щільність кісткової тканини великогомілкової кістки (в якій проводилася імплантація) при провокаційному типі вигодовування тварин за три місяців до та три місяці після імплантації [14].…”