Mineral deposition promoted by resin-based sealants with different calcium phosphate additions

Abstract

The aim of this study was to evaluate the influence of different calcium phosphates (CaPs) on the physical, biological, and remineralizing properties of experimental resin-based sealants (RBSs). Triethylene-glycol dimethacrylate (90wt%) and bisphenol A-glycidyl methacrylate (10wt%) were used to produce resin-based sealants. Hydroxyapatite (S HAp), α-tricalcium phosphate (Sα-TCP) and octacalcium phosphate (S OCP) were added to the sealants in a 10wt% concentration. One group without CaPs was used as the control group (S CG). The degree of conversion (DC) was assessed with Fourier-transformed infrared spectroscopy, whereas cytotoxicity was tested with the HaCaT keratinocyte cell line. The ultimate tensile strength (UTS) was used to assess the mechanical strength of the experimental RBSs. Sealed enamel was used for colorimetric assay. Mineral deposition was assessed with Raman spectroscopy after 7, 14, and 28 days of sample immersion in artificial saliva. Scanning electron microscopy was used to analyze the surface morphology after 28 days of immersion. The addition of 10wt% of fillers significantly reduced the DC of sealants. S OCP groups showed reduced cell viability. Higher UTS was found for Sα-TCP and S HAp. The color analysis showed that S GC and demineralized teeth presented higher mismatches with the sound tissue. Mineral deposition was observed for S HAp and Sα-TCP after 7 days, with increased phosphate content and mineral deposits for S HAp after 28 days. RBS with the addition of 10% HAp promoted increased mineralization in vitro after 28 days, and did not affect cell viability, DC, mechanical properties, or RBS color in the enamel.

Remineralizing Effects of Resin-Based Dental Sealants: A Systematic Review of In Vitro Studies

“…However, when the hardness was measured only for the material without measuring the baseline and the change in the hardness, it was considered a physical property, and was not included in this review. Furthermore, the reminreliaizng abilities was assessed using SEM-EDX analysis in two studies [33,44], and seven studies used SEM imaging [20,27,35,36,48,51,54]. There was a variation in the results between the studies.…”