Isolation and characterization of a human cementocyte-like cell line, HCY-23

Almeida, Amanda Bandeira de; Santos, Elis Janaína Lira dos; Abuna, Gabriel Flores; Salmon, Cristiane R.; Casati, Márcio Zaffalon; Silvério, Karina Gonzáles; Nociti, Francisco Humberto

doi:10.1590/1807-3107bor-2019.vol33.0058

Cited by 6 publications

(5 citation statements)

References 21 publications

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“…The collagen isoform collagen 1α is encoded by the gene COL1A1. It is characteristic of cementoblasts and constitutes the main part of the extracellular matrix of dental cementum [ 50 , 51 ]. The extracellular protein is upregulated both at mRNA and protein level after CAP treatment, indicating a CAP-dependent increase in the overall physiological activity of the cementoblasts.…”

Section: Discussionmentioning

confidence: 99%

Cold Atmospheric Plasma Promotes Regeneration-Associated Cell Functions of Murine Cementoblasts In Vitro

Eggers

Marciniak

Deschner

et al. 2021

IJMS

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The aim of the study was to examine the efficacy of cold atmospheric plasma (CAP) on the mineralization and cell proliferation of murine dental cementoblasts. Cells were treated with CAP and enamel matrix derivates (EMD). Gene expression of alkaline phosphatase (ALP), bone gamma-carboxyglutamate protein (BGLAP), periostin (POSTN), osteopontin (OPN), osterix (OSX), collagen type I alpha 1 chain (COL1A1), dentin matrix acidic phosphoprotein (DMP)1, RUNX family transcription factor (RUNX)2, and marker of proliferation Ki-67 (KI67) was quantified by real-time PCR. Protein expression was analyzed by immunocytochemistry and ELISA. ALP activity was determined by ALP assay. Von Kossa and alizarin red staining were used to display mineralization. Cell viability was analyzed by XTT assay, and morphological characterization was performed by DAPI/phalloidin staining. Cell migration was quantified with an established scratch assay. CAP and EMD upregulated both mRNA and protein synthesis of ALP, POSTN, and OPN. Additionally, DMP1 and COL1A1 were upregulated at both gene and protein levels. In addition to upregulated RUNX2 mRNA levels, treated cells mineralized more intensively. Moreover, CAP treatment resulted in an upregulation of KI67, higher cell viability, and improved cell migration. Our study shows that CAP appears to have stimulatory effects on regeneration-associated cell functions in cementoblasts.

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Section: Discussionmentioning

confidence: 99%

Cold Atmospheric Plasma Promotes Regeneration-Associated Cell Functions of Murine Cementoblasts In Vitro

Eggers

Marciniak

Deschner

et al. 2021

IJMS

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“…Recent evidence, added to our findings, also contributes to the understanding of the cementocyte as an active cell type in cementum physiology. A remodeling compartment in cementum was recently identified [32], as well as the in vitro responsiveness of cementocyte-like cells to inorganic phosphate has been described, demonstrating a potential role in the development of cementum [10].…”

Section: Resultsmentioning

confidence: 95%

“…Although there are some similarities between osteocytes and cementocytes, differentiation and functions remains poorly understood [8][9][10]. The function of cementocytes in periodontal physiology was not well established.…”

Section: Introductionmentioning

confidence: 99%

Systemic rosiglitazone administration leads to cementocytes apoptosis in wild type mice

et al. 2021

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It has been shown that a class of drugs for diabetes control, the thiazolidinediones, leads to increased apoptosis in osteocytes. Considering the correlations between osteocytes and cementocytes, the aim of this study was to demonstrate the apoptosis on cementocytes of wild type mice that had received rosiglitazone. Twenty-four male C57BL/6 mice were divided into 3 groups: 1 control, which received only the vehicle administration via oral for 1 week (PBS+DMSO 10%) and other two groups, which received 10 mg/kg of RGZ+PBS+DMSO 10% for 1 or 2 weeks, respectively. Upon completion of the time courses, mice were killed by CO2 and the mandibles were dissected and subjected to routine histotechnical processing. The sections were analyzed through transferase-mediated dUTP nick-end labeling (TUNEL) and 4’,6- diamidino-2-phenylindole (DAPI) staining of nuclear morphology (α=0.05). Control group showed significantly lower apoptotic cells/total cells ratio when compared to the experimental groups with TUNEL and DAPI methods (p=0.010 and 0.004, respectively). TUNEL method showed approximately 20% TUNEL-positive cementocytes in control and 26% in both experimental groups, while the DAPI technique showed approximately 32% of DAPI-positive cementocytes in control and 38% to 40% in experimental groups. The rosiglitazone systemic administration can lead to cementocytes apoptosis in mice. Despite the differences between the experimental and control groups, the death of cementocytes occurred as a physiological phenomenon, important in understanding the role of these cells in periodontal tissue.

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“…Regarding the cells from its structure, the cementocytes are phosphate-responsive cells, able to regulate cementum homeostasis. They express sclerostin, SERFINF1, DMP1, SOD3 and E11/gp38/podoplanin, but no markers for odontoblasts [109,113,114].…”

Section: Cellular Mixed Cementummentioning

confidence: 99%

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2022

Rom J Morphol Embryol

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This article is a review of new advances in histology, concerning either classification or structure of different tissular elements (basement membrane, hemidesmosomes, urothelium, glandular epithelia, adipose tissue, astrocytes), and various organs' constituents (blood-brain barrier, human dental cementum, tubarial salivary glands, hepatic stellate cells, pineal gland, fibroblasts of renal interstitium, Leydig testicular cells, ovarian hilar cells), as well as novel biotechnological techniques (tissue engineering in angiogenesis), recently introduced.

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Isolation and characterization of a human cementocyte-like cell line, HCY-23

Abstract: Amanda Bandeira de ALMEIDA (a) Elis Janaína Lira dos SANTOS (a) Gabriel Flores ABUNA (b) Cristiane Salmon RIBEIRO (a) Márcio Zaffalon CASATI (a) Karina Gonzales Silvério RUIZ (a)

Cited by 6 publications

References 21 publications

Cold Atmospheric Plasma Promotes Regeneration-Associated Cell Functions of Murine Cementoblasts In Vitro

Cold Atmospheric Plasma Promotes Regeneration-Associated Cell Functions of Murine Cementoblasts In Vitro

Systemic rosiglitazone administration leads to cementocytes apoptosis in wild type mice

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