In vitro evaluation of Eugenia dysenterica in primary culture of human gingival fibroblast cells

Abstract

In vitro evaluation of Eugenia dysenterica in primary culture of human gingival fibroblast cells Abstract: Eugenia dysenterica is a Brazilian tree investigated for its properties and bioactive compounds, which are believed to have both pharmacological and phytochemical therapeutic effects. The leaves of this tree contain tannins, flavonoids, terpenes, and saponins, with reportedly beneficial effects to the human body. Despite these therapeutic applications, its effects have never been tested on oral tissues. Therefore, the aim of the present study was to evaluate the cytotoxic and antioxidant effects and the anti-inflammatory and repair properties of the acetone fraction of E. dysenterica on primary culture of human gingival fibroblasts and on the immortalized murine macrophage cell line (RAW 264.7). For this purpose, a metabolic activity assay, a wound healing assay, a nitric oxide assay, and RT-qPCR were performed. The assays revealed a cytoprotective effect of this plant, suggested by the increase in the expression of SOD1 and NRF2. An antioxidant potential effect was observed in the DPPH• assay. However, the fraction of E. dysenterica did not show anti-inflammatory activity. In conclusion, Eugenia dysenterica may promote cytoprotection when associated with chlorhexidine digluconate because of its antioxidant effect. However, additional studies are necessary on other human dental tissues using other parts of the plant in order to develop a possible mouthwash to assist patients with oral disorders.

Nutraceutic Potential of Bioactive Compounds of Eugenia dysenterica DC in Metabolic Alterations

“…The antioxidant effect of E. dysenterica demonstrated the ability to promote cytoprotection when associated with chlorhexidine gluconate, which can be used to help patients with oral disorders by preventing lipoperoxidation and acetylcholine activity [51,52].…”

Antioxidantes producidos por microorganismos acuáticos y terrestres con uso potencial en cosméticos

“…Para ensayos toxicológicos se utilizan, por lo general, células del tejido epitelial como los fibroblastos, ya que brindan resultados más exactos de toxicidad para humanos y no exigen condiciones complejas de crecimiento; se las puede mantener en medio DMEM (Dulbeco's Modified Eagle's Medium) a 37 • C en una atmósfera húmeda con 5% de CO 2 (Huang et al, 2016). Además, para evaluar cosméticos destinados a la cavidad bucal u otros que presenten el riesgo de ingresar a la misma, se pueden utilizar fibroblastos gingivales, los cuales pueden cultivarse también en el medio DMEM a 37 • C en una atmósfera húmeda con 5% de CO 2 (Costa et al, 2019).…”

Impact of different types of storage media on enamel surface roughness and granularity distribution of avulsed teeth (In vitro study)

“…Natural products had been investigated as potential pharmacological substitutes, and as interest in complementary and alternative medicine has grown, numerous studies on the use of natural products for tissue healing had been carried out (7) . Because natural ingredients are easily available and have the ability to keep PDL cells alive for longer periods of time, they can be employed as storage media (8) .…”