P-glycoprotein expression in oral lichen planus

Abstract

Oral lichen planus (OLP) is a stress induced inflammatory condition with malignant potency. The mdr1 (multidrug resistance) is a stress gene overexpressed in cancerous conditions and its translated form, the p-glycoprotein efflux transporter is usually overexpressed with chemotherapy, leading to chemoresistance. OLP, a lesion with carcinogenic potency, is broadly classified into the asymptomatic reticular form and the aggressive erosive form. The objective of the study was to verify the expression level of p-glycoprotein in antifungaltreated and untreated reticular OLP, in untreated erosive OLP and erosive OLP patients treated with corticosteroid. Semi-quantitative reverse transcriptase polymerase chain reaction (SQ-RTPCR) and ELISA were performed on biopsy tissue samples to evaluate the mdr1 mRNA and protein expression of p-glycoprotein, respectively. The present study shows for the first time that mdr1 mRNA as well as its translated form p-glycoprotein are overexpressed in OLP subjects compared to healthy individuals. This overexpression is significantly higher in erosive than in reticular OLP patients, further confirming that the erosive form has higher risk for multidrug resistance. A higher expression is also observed in corticosteroid-treated erosive cases than similar untreated ones. The gradation of expression is in conformity with severity of the disease.

Relationship Between Amyloid-β Deposition and Blood–Brain Barrier Dysfunction in Alzheimer’s Disease

“…The expression and function of P-gP are compromised with age and in cases of AD ( Guerreiro and Bras, 2015 ). Aβ is considered to be a P-gP substrate ( Lam et al, 2001 ), and accumulating evidence supports its role in Aβ clearance from the brain ( Jana et al, 2017 ).…”

Photobiomodulation Improves Anti-Tumor Efficacy of Photodynamic Therapy against Resistant MCF-7 Cancer Cells

“…Cancer resistance has emerged as a threat because of the dynamic challenges of multidrug resistance, resulting from increased expression of P-gp that prevents and extrudes drugs from the cell [ 20 , 21 , 22 , 23 ]. Drug resistance stems from intrinsic and acquired mechanisms that often require a combination of therapies [ 10 ].…”

Response of MCF-7 Breast Cancer Cells Overexpressed with P-Glycoprotein to Apoptotic Induction after Photodynamic Therapy

“…Multidrug resistance is one of the main reasons why cancers prove challenging to treat. Most cancer cells with increased P-gp phenotype are resistant to chemotherapeutic treatment due to the P-gp membrane protein, which constantly extrudes harmful substances out of the cell [ 12 , 13 , 14 , 15 ]. The findings of the present study revealed that PDT on MCF-7/DOX cells with increased p-gp phenotype triggered the expression of caspase 8, Bax, and p53 proteins in the process of cell death.…”