Analysis of GLUT-1, GLUT-3, and angiogenic index in syndromic and non-syndromic keratocystic odontogenic tumors

Abstract

Analysis of GLUT-1, GLUT-3, and angiogenic index in syndromic and non-syndromic keratocystic odontogenic tumors Abstract: The aim of this study was to evaluate the immunoexpression of glucose transporters 1 (GLUT-1) and 3 (GLUT-3) in keratocystic odontogenic tumors associated with Gorlin syndrome (SKOTs) and non-syndromic keratocystic odontogenic tumors (NSKOTs), and to establish correlations with the angiogenic index. Seventeen primary NSKOTs, seven recurrent NSKOTs, and 17 SKOTs were selected for the study. The percentage of immunopositive cells for GLUT-1 and GLUT-3 in the epithelial component of the tumors was assessed. The angiogenic index was determined by microvessel count. The results were analyzed statistically using the nonparametric Kruskal-Wallis test and Spearman's correlation test. High epithelial immunoexpression of GLUT-1 was observed in most tumors (p = 0.360). There was a higher frequency of negative cases for GLUT-3 in all groups. The few GLUT-3-positive tumors exhibited low expression of this protein in epithelial cells. No significant difference in the angiogenic index was observed between groups (p = 0.778). GLUT-1 expression did not correlate significantly with the angiogenic index (p > 0.05). The results suggest that the more aggressive biological behavior of SKOTs when compared to NSKOTs may not be related to GLUT-1 or GLUT-3 expression. GLUT-1 may play an important role in glucose uptake by epithelial cells of KOTs and this process is unlikely related to the angiogenic index. GLUT-1 could be a potential target for future development of therapeutic strategies for KOTs.

Roles of GLUT-1 and HK-II expression in the biological behavior of head and neck cancer

“…Leite et al (2017) detected GLUT-1 and GLUT-3 expression in both keratocystic odontogenic tumors associated with Gorlin syndrome (SKOTs) and non-syndromic keratocystic odontogenic tumors (NSKOTs) by IHC. They revealed positive GLUT-1 expression in the epithelial component in all cases [78]. They found that GLUT-1 and GLUT-3 were not associated with the angiogenic index in SKOTs, primary NSKOTs, or recurrent NSKOTs [78].…”

“…They revealed positive GLUT-1 expression in the epithelial component in all cases [78]. They found that GLUT-1 and GLUT-3 were not associated with the angiogenic index in SKOTs, primary NSKOTs, or recurrent NSKOTs [78].…”

Immunoexpression of CXCL12 and CXCR4 in sporadic and Gorlin-Goltz syndrome-related odontogenic keratocysts

“…This syndrome is an autosomal dominant inherited disorder characterized by the presence of multiple OKCs, basal cell carcinomas distributed throughout the body, and bone malformations ( 6 ). Studies have suggested syndromic OKCs are potentially more aggressive ( 7 ), with higher probability of growth and expansion and higher recurrence when compared with sporadic OKCs ( 4 , 5 ).…”

“…Numerous proteins, including those related to apoptosis ( 7 ), innate immune response ( 8 ), glucose transport ( 5 ), and bone resorption ( 4 ), have been investigated in order to identify the differences in biological behavior between OKCs associated with Gorlin-Goltz syndrome and sporadic OKCs. Even though these investigations have contributed to understanding the pathogenesis of these lesions, the mechanisms underlying these differences between sporadic and syndromic OKCs have not been fully elucidated.…”

“…OKC is possibly associated with Gorlin-Goltz syndrome ( 4 , 5 ). This syndrome is an autosomal dominant inherited disorder characterized by the presence of multiple OKCs, basal cell carcinomas distributed throughout the body, and bone malformations ( 6 ).…”