Evaluation of fine needle aspiration biopsy in oral cavity and head and neck region with different stains techniques

Santos, Ana Paula Candido dos; Sugaya, Norberto Nobuo; Pinto, Décio dos Santos; Lemos, Celso Augusto

doi:10.1590/1807-3107bor-2015.vol29.0080

Cited by 5 publications

(6 citation statements)

References 18 publications

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“…HE staining can also be used, mainly in CB preparations. 27 In our study, all cytological smears from FNAC were stained with Panoptic and Papanicolaou because these stains are used routinely in cytology. HE was the third most common stain, performed in 574 cases (53.0%).…”

Section: Discussionmentioning

confidence: 99%

“…Santos et al 27 Grocott-Gomori methenamine silver stain (n = 2;0.2%). Associating EC and FNAC with stains, there was a positive correlation between PAS with EC (P > .001) and between HE with CB preparations from FNAC (P < .001).…”

Section: Discussionmentioning

confidence: 99%

“…Panoptic staining is used in air‐dried smears and Papanicolaou in slides fixed in 95% alcohol. HE staining can also be used, mainly in CB preparations 27 . In our study, all cytological smears from FNAC were stained with Panoptic and Papanicolaou because these stains are used routinely in cytology.…”

Section: Discussionmentioning

confidence: 99%

“…HE was the third most common stain, performed in 574 cases (53.0%). Santos et al 27 evaluated the different stain techniques in FNAC; they found that HE stain showed better accuracy to provide a definitive diagnosis, followed by Papanicolaou and Panoptic stains. Slides fixed in alcohol and formalin, such as those used for Papanicolaou and HE stain, presented better preservation of cell morphologies.…”

Section: Discussionmentioning

confidence: 99%

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An audit of cytopathology in the oral and maxillofacial region: 18 years of experience

et al. 2020

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Introduction: The aim of this study was to perform an audit of oral and maxillofacial specimens submitted for cytological diagnosis to verify the importance of this complementary examination. Methods: A retrospective analysis of our institutional cytopathology database was performed over an 18-year period. Clinical information and cytological data were collected. Associations between independent variables and outcomes were assessed using the Pearson χ 2 test or Fisher's test, with a 5% significance level. When available, the histological diagnosis was compared with cytological diagnosis to identify the percentage of agreement and the specificity, sensitivity and accuracy of cytology in identifying malignant neoplasms. Results: A total of 1082 cases were identified, which included 65 different cytological diagnoses. Exfoliative cytology (EC) was performed in 312 cases (29.1%) and fine needle aspiration cytology (FNAC) in 770 cases (70.9%). EC was mainly employed to diagnose oral infectious diseases (P < 0.001) and FNAC to diagnose neoplasms, cystic, reactive and miscellaneous lesions (P < 0.001). Cell-block was performed in 555 FNAC cases (51.3%). Panoptic, Papanicolaou and haematoxylin-eosin staining were performed in FNAC and periodic acid-Schiff in EC (P < 0.001). In 211 cases (19.5%), the histological diagnosis was available and the percentage agreement with the cytological diagnosis was 41.2%. Sensitivity, specificity, positive predictive value, negative predictive value and accuracy to identify malignant neoplasms were 84.6%, 100%, 100%, 77.8% and 90.0%, respectively. Conclusions: EC was mainly performed for diagnosis of infectious diseases and FNAC for diagnosis of salivary gland tumours, odontogenic lesions, reactive lesions and cervical metastasis. K E Y W O R D S cytopathology, exfoliative cytology, fine needle aspiration cytology, head and neck, oral cavity PÉREZ-DE-OLIVEIRA Et AL. 1 | INTRODUC TI ON Oral diseases can share many clinical aspects, and complementary tests may be needed to achieve a diagnosis. Cytology represents a diagnostic tool based on a microscopic evaluation of the cell samples collected from mucosal surface via exfoliative cytology (EC) or internal sites via fine needle aspiration cytology (FNAC). 1 This complementary examination is widely used in many clinical routines, such as investigation of masses in lymph nodes, liver, thyroid and breast, as well as screening of precursor lesions of cervical carcinoma and follow-up of patients with lung carcinoma. 2,3 In oral diagnosis, the use of cytology examination remains controversial. Previous reports demonstrated that cytopathology can be a useful tool in head and neck lesions. 3,4 Most reports have focused on the thyroid, 5 major salivary gland 6 and cervical lymph node masses, 7 in which, when well indicated, performed and interpreted, cytopathology can achieve a high accuracy to allow for correct therapeutic management. Single reports in the literature suggest that cytopathology applications can go beyond the above-mentioned scenario...

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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An audit of cytopathology in the oral and maxillofacial region: 18 years of experience

et al. 2020

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“…Panoptic staining is used in air-dried smears and Papanicolaou in slides fixed in 95% alcohol. HE staining can also be used, mainly in CB preparations, 27 In our study, all cytological smears from FNAC were stained with Panoptic and Papanicolaou because these stains are used routinely in cytology. HE stain was the third most common stain, performed in 574 cases (53.0%).…”

Section: Discussionmentioning

confidence: 99%

Aplicação da citopatologia no diagnóstico da lesões orais e maxilofaciais

Hernandez¹

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Márcio Ajudarte Lopes.O presente trabalho foi realizado com apoio da Coordenação de Aperfeiçoamento de Pessoal de Nível Superior -Brasil (CAPES) -Código de Financiamento 001.Ao meu orientador, Prof. Dr. Pablo Agustin Vargas, pelos ensinamentos e oportunidades proporcionadas.À minha co-orientadora, Dra. Vivian Petersen Wagner, pelo apoio à elaboração desse trabalho e pelo carinho me dado nessa trajetória.A todos os amigos da pós-graduação, pela convivência e parceria. A rede de apoio que conseguimos criar é, sem dúvidas, essencial para nosso crescimento pessoal e profissional. Serei eternamente grata por tudo.Aos meus pais, Fernanda e Eduardo, por me proporcionar suporte e amor que são fundamentais para que eu possa ir em busca dos meus objetivos. Agradeço toda confiança depositada em mim. À minha irmã, Maria Amanda, por me mostrar o real significado da irmandade e por ser uma inspiração e uma fortaleza para mim.Ao meu noivo e grande amigo, Matheus, por seu apoio e incentivo incondicionais, sempre acreditando no meu potencial.Aos meus verdadeiros amigos que, mesmo distantes fisicamente, sei que estarão sempre torcendo pelo meu sucesso. Cativo cada um de vocês dentro do meu coração. RESUMOO objetivo desse estudo foi realizar um levantamento dos casos submetidos para o diagnóstico citopatológico para demonstrar as diversas aplicações desse exame complementar na região oral e maxilofacial. Uma análise retrospectiva foi realizada no Laboratório de Patologia Oral da Faculdade de Odontologia de Piracicaba num período de 18 anos. Informações clínicas e citológicas foram coletadas. Associações entre as variáveis clínicas e citológicas foram realizadas através do teste do qui-quadrado de Pearson ou teste de Fisher, com um nível de significância de 5%. Quando disponível, o diagnóstico histopatológico foi comparado com o diagnóstico citológico para identificar a porcentagem de concordância entre esses exames e foram calculadas a especificidade, sensibilidade e acurácia da citopatologia em identificar neoplasias malignas. 1.082 casos foram identificados compreendendo 65 diferentes diagnósticos. A citologia esfoliativa (CE) foi realizada em 312 casos (29,1%) e a punção aspirativa por agulha fina (PAAF) em 770 casos (70.9%). CE foi empregada principalmente para diagnóstico de lesões infecciosas e PAAF para diagnósticos do grupo de neoplasias, lesões císticas, reacionais e de patologia variada. Cell Block (CB) foi realizado em 555 casos (51.3%) derivados da PAAF. Colorações de Panótico, Papanicolaou e hematoxilina-eosina foram realizadas na PAAF e Ácido periódico de Schiff na EC. Em 211 casos (19.5%) o diagnóstico histopatológico estava disponível e a porcentagem de concordância com o diagnóstico citopatológico foi de 40.3%. Sensibilidade, especificidade, valor preditivo positivo, valor preditivo negativo e acurácia da citologia em identificar neoplasias malignas foi 84.6%, 100%, 77.8% e 90.0%, respectivamente. Em conclusão, a Citopatologia forneceu diferentes diagnósticos de lesões da região de cabeça e pescoço. A CE foi realiz...

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Three-dimensional Pattern of Inflammatory Periapical Lesion Extension in the Premolar’s Region: An Application of K-means Clustering

Kazemipoor

Valizadeh

Jambarsang

2021

CMIR

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Background: Cone-beam computed tomography (CBCT) provides better diagnosis of endodontic lesions. Introduction: The present study would assess the pattern of periapical lesion extension in premolar teeth using CBCT. Method: In this descriptive study‚ 330 roots in the regions of maxillary and mandibular premolars have been evaluated. Maximum periapical lesion extensions in the three orthogonal planes (axial, coronal and sagittal) were measured and recorded in millimeters. Measurements were compared based on gender‚ dental arch, tooth type and root. Statistical analysis was performed using repeated measure ANOVA, Bonferroni, Chi-square tests and clustering data analysis (K-means method). The significant level was set at 0.05. Result: There were significant differences between the lesion expansions in the three dimensional planes (p-value<0.001). The highest average of lesion extension in the premolar regions of the examined population was reported in the vertical dimension (4.1± 1.3), followed by horizontal buccolingual dimension (3.4±1.1) and horizontal mesiodistal dimension (3.1±1.0) respectively. According to independent variables, in the premolar region only tooth roots showed significant differences in the lesion extension (p-value=0.002). Clustering data analysis showed that the majority of the participants categorized in a cluster with lower lesion extension. Based on clustering data analysis, the small lesions were significantly observed in the first premolar and buccal roots. Conclusion: Since the periapical lesion extension in the buccolingual dimension, which could not be detected in the 2-D imaging techniques, was rather high in the region of premolar teeth, CBCT -as a 3-D imaging technique- is a suitable option for the precise evaluation of periapical lesion extension. Also, the majority of the lesions in this tooth area are small and located in the buccal roots.

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Evaluation of fine needle aspiration biopsy in oral cavity and head and neck region with different stains techniques

Cited by 5 publications

References 18 publications

An audit of cytopathology in the oral and maxillofacial region: 18 years of experience

An audit of cytopathology in the oral and maxillofacial region: 18 years of experience

Aplicação da citopatologia no diagnóstico da lesões orais e maxilofaciais

Three-dimensional Pattern of Inflammatory Periapical Lesion Extension in the Premolar’s Region: An Application of K-means Clustering

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