Decalcifying efficacy of different irrigating solutions: effect of cetrimide addition

Abstract

The objective of the present study was to evaluate and compare the influence of cetrimide on decalcifying capability of different irrigating solutions. Fifteen maxillary central incisor teeth has been collected. The canals were prepared in order to obtain four samples from each root. The specimens were randomly divided into 6 experimental groups (n=10) according to tested irrigating agents. Irrigating agents consisted in different composition of EDTA and citric acid solutions, addicted or not with cetrimide. Each specimen was submitted to three successive 5-min immersions in each solution. After exposures, the concentration of Ca2+ extracted was measured by inductively coupled plasma-atomic emission spectrometry (ICP-AES). Data were analysed by means of Kruskal Wallis and Mann Whitney tests. Significance was predetermined at p < 0.05. For all irrigants, the amounts of Ca2+ extracted from root canal dentin samples at 10 minutes were not significantly different from values reported after 15 minutes respectively. Therefore, for all irrigants tested, 10 minutes of application are sufficient to obtain maximum Ca2+ release. Moreover citric acid based agents observed a higher release of Ca2+. The addition of cetrimide did not affect the decalcifying capability of the EDTA and citric acid solutions.

Decalcifying capability of irrigating solutions on root canal dentin mineral content

“…The addition of 1% cetrimide did not affect the extraction properties of the EDTA and citric acid solutions because the values of concentration of Ca 2+ released in the two solutions did not significantly differ. [ 26 ] Higher amount of Ca 2+ extracted from root dentin in the present study is due at a greatest concentration of citric acid and surfactants of Tetraclean NA than Tetraclean as confirmed in the previous study. [ 26 ] It indicates that to obtain an efficient smear layer removal and to facilitate the biomechanical procedures, citric acid-based agents can be applied and cetrimide can help in improving efficacy of the irrigating solutions, moreover cetrimide does not affect the ability of demineralization of citric acid and EDTA solutions.…”

“…[ 26 ] Higher amount of Ca 2+ extracted from root dentin in the present study is due at a greatest concentration of citric acid and surfactants of Tetraclean NA than Tetraclean as confirmed in the previous study. [ 26 ] It indicates that to obtain an efficient smear layer removal and to facilitate the biomechanical procedures, citric acid-based agents can be applied and cetrimide can help in improving efficacy of the irrigating solutions, moreover cetrimide does not affect the ability of demineralization of citric acid and EDTA solutions. Cationic surfactants are potent antimicrobial agents that have also been shown to act on biofilm components, but they have no decalcifying effects on root canal dentin.…”

Desmineralización de la Dentina Humana Producida por Irrigantes Endodónticos Combinados

“…Estudios demostraron alteraciones de la microdureza (López et al, 2015), resistencia a la flexión, módulo de elasticidad (Gu et al, 2017), permeabilidad y solubilidad (Uzunoglu et al, 2012). Así mismo la modificación del radio de Ca2+/PO43 podría modificar la habilidad de sellado y adhesión de los cementos utilizados para la obturación posterior del conducto (Poggio et al, 2014).…”