Evaluation of dentists’ knowledge of the use of oral exfoliative cytology in clinical practice

Silva, Weber Adorno; Lima, Ana Paula de; Vasconcellos, Luana Marotta Reis de; Anbinder, Ana Lia

doi:10.1590/1807-3107bor-2014.vol28.0010

Cited by 3 publications

(3 citation statements)

References 17 publications

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“…The use of cytopathology in the head and neck region is well recognised; however, in the oral cavity it remains controversial. 3 Silva et al 15 highlighted the need to increase dentists' knowledge of EC and its use in diagnostic practices. The broad diagnoses of histopathological examination in oral and maxillofacial region have been previously investigated 14,16 ; however, no study was conducted to determine the uses of EC and FNAC in this region.…”

Section: Discussionmentioning

confidence: 99%

An audit of cytopathology in the oral and maxillofacial region: 18 years of experience

et al. 2020

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Introduction: The aim of this study was to perform an audit of oral and maxillofacial specimens submitted for cytological diagnosis to verify the importance of this complementary examination. Methods: A retrospective analysis of our institutional cytopathology database was performed over an 18-year period. Clinical information and cytological data were collected. Associations between independent variables and outcomes were assessed using the Pearson χ 2 test or Fisher's test, with a 5% significance level. When available, the histological diagnosis was compared with cytological diagnosis to identify the percentage of agreement and the specificity, sensitivity and accuracy of cytology in identifying malignant neoplasms. Results: A total of 1082 cases were identified, which included 65 different cytological diagnoses. Exfoliative cytology (EC) was performed in 312 cases (29.1%) and fine needle aspiration cytology (FNAC) in 770 cases (70.9%). EC was mainly employed to diagnose oral infectious diseases (P < 0.001) and FNAC to diagnose neoplasms, cystic, reactive and miscellaneous lesions (P < 0.001). Cell-block was performed in 555 FNAC cases (51.3%). Panoptic, Papanicolaou and haematoxylin-eosin staining were performed in FNAC and periodic acid-Schiff in EC (P < 0.001). In 211 cases (19.5%), the histological diagnosis was available and the percentage agreement with the cytological diagnosis was 41.2%. Sensitivity, specificity, positive predictive value, negative predictive value and accuracy to identify malignant neoplasms were 84.6%, 100%, 100%, 77.8% and 90.0%, respectively. Conclusions: EC was mainly performed for diagnosis of infectious diseases and FNAC for diagnosis of salivary gland tumours, odontogenic lesions, reactive lesions and cervical metastasis. K E Y W O R D S cytopathology, exfoliative cytology, fine needle aspiration cytology, head and neck, oral cavity PÉREZ-DE-OLIVEIRA Et AL. 1 | INTRODUC TI ON Oral diseases can share many clinical aspects, and complementary tests may be needed to achieve a diagnosis. Cytology represents a diagnostic tool based on a microscopic evaluation of the cell samples collected from mucosal surface via exfoliative cytology (EC) or internal sites via fine needle aspiration cytology (FNAC). 1 This complementary examination is widely used in many clinical routines, such as investigation of masses in lymph nodes, liver, thyroid and breast, as well as screening of precursor lesions of cervical carcinoma and follow-up of patients with lung carcinoma. 2,3 In oral diagnosis, the use of cytology examination remains controversial. Previous reports demonstrated that cytopathology can be a useful tool in head and neck lesions. 3,4 Most reports have focused on the thyroid, 5 major salivary gland 6 and cervical lymph node masses, 7 in which, when well indicated, performed and interpreted, cytopathology can achieve a high accuracy to allow for correct therapeutic management. Single reports in the literature suggest that cytopathology applications can go beyond the above-mentioned scenario...

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Section: Discussionmentioning

confidence: 99%

An audit of cytopathology in the oral and maxillofacial region: 18 years of experience

et al. 2020

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“…Exfoliative cytology has become popular as it is noninvasive and time-effective. [ 1 ] This test can be used to diagnose various oral diseases, such as squamous cell carcinoma, potentially malignant disorders, candidiasis, and hairy leukoplakia, as well as to assist in monitoring dysplastic areas. [ 1 2 ] Exfoliative cytology uses the scrapings of different areas of the oral cavity such as the gingiva and buccal mucosa to detect changes of the superficial epithelial cells.…”

Section: Introductionmentioning

confidence: 99%

Comparative assessment of exfoliated gingival cells in healthy individuals and chronic periodontitis patients with and without tobacco chewing habit: A cytomorphometric study

Naik¹,

Nayak²,

Kottrashetti³

et al. 2022

J Indian Soc Periodontol

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Background: Cytomorphometry is the analysis of the morphometry of exfoliated cells. Studies on exfoliated gingival epithelial cells in chronic periodontitis were not evident on the literature search. In the backdrop of this information, we were inspired to conduct a cytomorphometric study. Aim: The aim of the study is to correlate the nuclear area (NA), cytoplasm area (CA), and the nuclear: cytoplasm ratio (N/C) in the gingival cells of healthy individuals, patients with chronic periodontitis, and chronic periodontitis patients who are tobacco chewers. Materials and Methods: Ninety subjects, aged 35–45 years, were categorized into three equal groups of 30 each. Group I had individuals with healthy gingiva, Group II patients with chronic periodontitis without any deleterious habits, while Group III patients with chronic periodontitis and tobacco chewing habit. Exfoliated gingival cells were stained with Papanicolaou stain. Analysis of cells was done using binocular light microscope under ×40 and i-progress ® image analyzer software. Results: There was statistically significant increase in CA in all the groups, but NA and N/C ratio did not show any statistical significant difference. When Group II was compared with Group III, we found significant correlation of CA with pocket probing depth and clinical attachment loss (CAL) in Group II. Similarly, we also found a correlation of CA and N/C with pocket depth and CA with CAL in Group III. Conclusion: The results suggest that molecular level changes occur in the cells. Cytomorphometry can be used to analyze the cellular changes in chronic periodontitis patients even before clinical signs manifest. Hence, it can be used as an investigative tool.

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“…The use of cytopathology in the head and neck region has been proposed; however, in the oral cavity it remains controversial. 3 Silva et al 15 highlighted the need to increase dentists' knowledge of EC and its use in diagnostic practices. The broad diagnoses of histopathological exam in oral and maxillofacial region have been previously investigated 14,16 ; however, no study was conducted to determine the uses of EC and FNAC in this region.…”

Section: Discussionmentioning

confidence: 99%

Aplicação da citopatologia no diagnóstico da lesões orais e maxilofaciais

Hernandez¹

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Márcio Ajudarte Lopes.O presente trabalho foi realizado com apoio da Coordenação de Aperfeiçoamento de Pessoal de Nível Superior -Brasil (CAPES) -Código de Financiamento 001.Ao meu orientador, Prof. Dr. Pablo Agustin Vargas, pelos ensinamentos e oportunidades proporcionadas.À minha co-orientadora, Dra. Vivian Petersen Wagner, pelo apoio à elaboração desse trabalho e pelo carinho me dado nessa trajetória.A todos os amigos da pós-graduação, pela convivência e parceria. A rede de apoio que conseguimos criar é, sem dúvidas, essencial para nosso crescimento pessoal e profissional. Serei eternamente grata por tudo.Aos meus pais, Fernanda e Eduardo, por me proporcionar suporte e amor que são fundamentais para que eu possa ir em busca dos meus objetivos. Agradeço toda confiança depositada em mim. À minha irmã, Maria Amanda, por me mostrar o real significado da irmandade e por ser uma inspiração e uma fortaleza para mim.Ao meu noivo e grande amigo, Matheus, por seu apoio e incentivo incondicionais, sempre acreditando no meu potencial.Aos meus verdadeiros amigos que, mesmo distantes fisicamente, sei que estarão sempre torcendo pelo meu sucesso. Cativo cada um de vocês dentro do meu coração. RESUMOO objetivo desse estudo foi realizar um levantamento dos casos submetidos para o diagnóstico citopatológico para demonstrar as diversas aplicações desse exame complementar na região oral e maxilofacial. Uma análise retrospectiva foi realizada no Laboratório de Patologia Oral da Faculdade de Odontologia de Piracicaba num período de 18 anos. Informações clínicas e citológicas foram coletadas. Associações entre as variáveis clínicas e citológicas foram realizadas através do teste do qui-quadrado de Pearson ou teste de Fisher, com um nível de significância de 5%. Quando disponível, o diagnóstico histopatológico foi comparado com o diagnóstico citológico para identificar a porcentagem de concordância entre esses exames e foram calculadas a especificidade, sensibilidade e acurácia da citopatologia em identificar neoplasias malignas. 1.082 casos foram identificados compreendendo 65 diferentes diagnósticos. A citologia esfoliativa (CE) foi realizada em 312 casos (29,1%) e a punção aspirativa por agulha fina (PAAF) em 770 casos (70.9%). CE foi empregada principalmente para diagnóstico de lesões infecciosas e PAAF para diagnósticos do grupo de neoplasias, lesões císticas, reacionais e de patologia variada. Cell Block (CB) foi realizado em 555 casos (51.3%) derivados da PAAF. Colorações de Panótico, Papanicolaou e hematoxilina-eosina foram realizadas na PAAF e Ácido periódico de Schiff na EC. Em 211 casos (19.5%) o diagnóstico histopatológico estava disponível e a porcentagem de concordância com o diagnóstico citopatológico foi de 40.3%. Sensibilidade, especificidade, valor preditivo positivo, valor preditivo negativo e acurácia da citologia em identificar neoplasias malignas foi 84.6%, 100%, 77.8% e 90.0%, respectivamente. Em conclusão, a Citopatologia forneceu diferentes diagnósticos de lesões da região de cabeça e pescoço. A CE foi realiz...

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Evaluation of dentists’ knowledge of the use of oral exfoliative cytology in clinical practice

Cited by 3 publications

References 17 publications

An audit of cytopathology in the oral and maxillofacial region: 18 years of experience

An audit of cytopathology in the oral and maxillofacial region: 18 years of experience

Comparative assessment of exfoliated gingival cells in healthy individuals and chronic periodontitis patients with and without tobacco chewing habit: A cytomorphometric study

Aplicação da citopatologia no diagnóstico da lesões orais e maxilofaciais

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