Assessment of the effects of decontamination and storage methods on the structural integrity of human enamel

Freitas, Adriana Rodrigues de; Aznar, Fábio Duarte da Costa; Silva, André Luís da; Sales-Peres, Arsênio; Sales-Peres, Sílvia Helena de Carvalho

doi:10.1590/1807-2577.05915

Cited by 6 publications

(6 citation statements)

References 26 publications

(29 reference statements)

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“…Teeth were rinsed thoroughly with running water and cleaned with soft brush after extraction to remove the soft debris from their surfaces. Teeth then were stored in 0.1% thymol (5) .…”

Section: Collection Of Teethmentioning

confidence: 99%

Assessment of Remineralizing Effect of Bioactive Glass Based Toothpastes: an In-Vitro Comparative Study

2021

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Purpose:The purpose of the study was to assess the enamel remineralization potential of two bioactive glass-based toothpastes: Biomin ® based toothpaste in comparison with Novamin ® based toothpaste with sodium fluoride. Material and methods: Forty premolars were selected and divided equally into two groups named Novamin and Biomin groups after discarding their roots. First, the samples were soaked in a demineralizing solution for three days to get white spot lesion. Then, after rinsing them with deionized water, toothpastes were applied in the form of slurry twice daily, for two minutes each. The remineralization process lasted for two weeks. Environmental Scanning Electron Microscope (ESEM) was used to assess the remineralization degree.It was attached to Energy-dispersive X-ray spectroscopy (EDX) unit to measure the Ca, P weight percent and also the Ca: P for each sample. Three EDX readings were taken for each sample; baseline, after demineralization and after remineralization. Results: The results showed that both toothpastes have remineralization potential without statistically significant difference between them except in phosphate weight percent which was higher in the Biomin group. Conclusions: Within the limitations of the present study we concluded that the mineral content of the demineralized enamel significantly increase with using both Biomin and Novamin containing toothpastes twice daily for two weeks. Biomin may be more effective than Novamin in remineralization of demineralized enamel.

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“…Teeth were rinsed thoroughly with running water and cleaned with soft brush after extraction to remove the soft debris from their surfaces. Teeth then were stored in 0.1% thymol (5) .…”

Section: Collection Of Teethmentioning

confidence: 99%

Assessment of Remineralizing Effect of Bioactive Glass Based Toothpastes: an In-Vitro Comparative Study

2021

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“…The teeth were extracted in the context of the treatment plan, and all patients gave written informed consent for the use of their extracted teeth for scientific research. After extraction, the teeth were scaled to remove organic and inorganic debris and stored in 0.1% thymol solution for 1 week for disinfection (ISO TS11405:2003 specifications) [28,29]. To prevent dehydration, the samples were kept in distilled water until they were used [28,30].…”

Section: Specimen Preparationmentioning

confidence: 99%

The Effect of Finishing and Polishing Sequences on The Surface Roughness of Three Different Nanocomposites and Composite/Enamel and Composite/Cementum Interfaces

et al. 2020

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The purpose of this study was to investigate the effect of final surface treatment and dental composite type on the roughness of the composite surface, composite/enamel interface, and composite/cementum interface, as well as on the polishing time. Class V cavities prepared in extracted teeth (n = 126) were restored using one of the three nanohybrid composites with different filler sizes. The specimens were randomly assigned to three different finishing and polishing sequences. The roughness (Ra) of the investigated surfaces was measured using the contact profilometer. The time required to achieve visible gloss was documented. The data were analyzed using ANOVA with Tukey’s post hoc test (p < 0.05). There was no significant influence of the composite type on the restoration surface roughness (p = 0.088), while the polishing method had a significant impact (p < 0.001). The Ra of the composites ranged between 0.08 µm and 0.29 µm, with the lowest values (0.09 µm ± 0.05 µm) found in the aluminum oxide disc group (p < 0.001). The time to achieve a visible composite gloss was influenced by the polishing method, composite type, and interactions between these factors (p < 0.001). The interface roughness was significantly greater than that of the composite surface (p < 0.001), and depended on the composite type and polishing system employed.

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“…It is important to note that distilled water does not remove the organic compounds after 30 days of immersion [ 39 ]. Immersion in 0.1% thymol solution has shown a decline compared to the laser fluorescence values [ 40 ]. The area under the ROC curve (AUROC) can be defined as a composite measure of accuracy.…”

Section: Discussionmentioning

confidence: 99%

Effectiveness of Visual-Tactile Examination and DIAGNOdent Pen in Detecting Early Enamel Caries and Its Remineralisation: An In Vitro Study

Rashid

Karobari

Halim

et al. 2022

BioMed Research International

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Background. The caries preventive effect of Colgate Duraphat® and GC Tooth Mousse Plus® has been widely studied, but the remineralisation potential of initial occlusal caries using these two remineralisation materials remains unclear. Aim. This study is aimed at evaluating and comparing the remineralisation of early enamel caries on the occlusal surface of permanent posterior teeth using ICDAS II caries scoring system and DIAGNOdent Pen (DDPen) after remineralisation with Colgate Duraphat® and GC Tooth Mousse Plus®. Materials and Methods. Extracted posterior teeth ( N = 120 ) with incipient occlusal caries were included in this study. The occlusal surface of each tooth was scored using DDPen and ICDAS II scoring before remineralisation. Then, remineralisation of the teeth of the experimental group was carried out using either CPP-ACP-F or fluoride varnish. After the remineralisation procedures, the occlusal surface of each tooth was again scored using DDPen and ICDAS II scoring. The teeth were then fixed in dental stone blocks and sectioned longitudinally for histological examination using a stereomicroscope. Statistical analysis was performed to calculate the sensitivity and specificity of DDPen and ICDAS II to detect remineralisation and compare with the gold standard histological examination. Results. According to ICDAS-II scores, a significant difference was noted in GC Tooth Mousse Plus® and Duraphat® study samples, whereas the difference between the pre-and post-remineralisation of the control group was not significant. According to the DDPen score criteria, a statistically significant difference was noted among all study groups; however, a greater significance level was noted in the GC Tooth Mousse Plus® and Duraphat® study samples compared with the control group. The Spearman’s rank correlation of ICDAS-II and DDPen with Downer’s histological score (gold standard) revealed a higher association of DDPen score (.738) as compared to ICDAS-II scores (.430). Conclusion. The study concluded that both ICDAS II and DDPen could detect remineralisation of early enamel occlusal caries. DDPen was more sensitive than ICDAS-II to detect remineralisation compared with the Downers histological scores.

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Assessment of the effects of decontamination and storage methods on the structural integrity of human enamel

Cited by 6 publications

References 26 publications

Assessment of Remineralizing Effect of Bioactive Glass Based Toothpastes: an In-Vitro Comparative Study

Assessment of Remineralizing Effect of Bioactive Glass Based Toothpastes: an In-Vitro Comparative Study

The Effect of Finishing and Polishing Sequences on The Surface Roughness of Three Different Nanocomposites and Composite/Enamel and Composite/Cementum Interfaces

Effectiveness of Visual-Tactile Examination and DIAGNOdent Pen in Detecting Early Enamel Caries and Its Remineralisation: An In Vitro Study

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