The use of high-quality seeds is a premise for the implantation of pasture for the generation of sustainable farming systems in livestock production. A usual practice is to mix brachiaria seeds with nitrogenous or phosphate fertilizers to sown and sidedressing. The objective of this study was to study the effect of contact time (0, 3, 12, 24, 48 and 72 hours) of urea mineral fertilizer with Brachiaria brizantha cv. Marandu high-quality seeds, in its physiological quality (germination and vigor). A completely randomized experimental design with additional treatment (seeds without fertilizer) was used, with three replicates, in laboratory and chamber conditions. Percentage of seedling emergence, germination of normal and abnormal seedlings and dead seeds were evaluated. Seeds were sown over the papers placed in gerboxes under controlled conditions of photoperiod and temperature (35°C for 8 hours of light and 20°C for 16 in dark conditions). It was found that after 72 hours of contact, all seeds lose its viability. The number of dead seeds presented significant (p <0.001) and negative correlation of 98.63% for the number of normal seedlings. The mix ratio between urea and seed affects the rate of seed deterioration, expressed as the lowest percentage of seedlings emerged in the sand, in relation to seeds not in contact with urea (control). It is concluded that from the reduction in viability and vigor begin after 3 hours of contact with fertilizers, so the number of unviable seeds highly increase after 48-hours contact.