Worse prognosis in breast cancer patients can be predicted by immunohistochemical analysis of positive MMP-2 and negative estrogen and progesterone receptors

Ramos, Edneia A.S.; Silva, Camila T da; Manica, Graciele Cristiane More; Pereira, Isabela Tiemy; Klassen, liliane Maria Bacaro; Ribeiro, E.; Cavalli, Iglenir João; Braun-Prado, Karin; Lima, Rubens Silveira de; Úrban, Cícero; Noronha, Lúcia de; Klassen, Giseli

doi:10.1590/1806-9282.62.08.774

Cited by 8 publications

(5 citation statements)

References 23 publications

(34 reference statements)

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“…Estrogen is a major driver of breast tumor cell growth (35) and ERα + breast cancer is the leading cause of breast cancer-associated mortality (36). ER and PR, along with matrix metalloproteinase-2, have a prognostic value in patients with breast cancer (37). Indeed, the ER and PR status of human breast cancer represent important prognostic and predictive markers for human breast cancer (38,39).…”

Section: Discussionmentioning

confidence: 99%

Overexpression of collagen type V α1 chain in human breast invasive ductal carcinoma is mediated by TGF-β1

Ren

Zhang

et al. 2018

Int J Oncol

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Collagen type V α1 chain (COL5A1) is a minor fibrillar collagen in mammals that co-polymerizes with type I collagen to adjust the diameter of collagen molecules. However, the function of COL5A1 in invasive ductal carcinoma (IDC) of the human breast remains unknown. In the present study, our group examined the expression of COL5A1 in IDC compared with its adjacent normal tissue and fibroadenoma of the breast. COL5A1 was revealed to be overexpressed in IDC compared with benign tumor and adjacent normal control tissues, and was associated with the expression of estrogen receptor and progesterone receptor. No association between COL5A1 expression and tumor size, lymph node metastasis, clinical stage, age, or Her2 expression was identified. High expression of COL5A1 mRNA was associated with distant metastasis free survival in patients with breast cancer. Knockdown of COL5A1 led to a decrease of cell viability, as detected by the WST-1 assay, and an inhibition of migration and invasion, as detected by wound healing and Transwell assays, respectively, in the breast cancer cell line MCF-7. The expression of COL5A1 in MCF-7 cells was downregulated by transforming growth factor (TGF)‑β1, which was abolished in the presence of SB-431542, an inhibitor of TGF-β type I receptor. In conclusion, these data indicated that COL5A1 is overexpressed in IDC and regulated by TGF-β1, suggesting that an increase of COL5A1 reflects tumor progression and may serve as a novel biomarker and therapeutic target for the treatment of breast IDC.

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Section: Discussionmentioning

confidence: 99%

Overexpression of collagen type V α1 chain in human breast invasive ductal carcinoma is mediated by TGF-β1

Ren

Zhang

et al. 2018

Int J Oncol

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“…The MMPs are involved in the key ECM degradation of proteolytic enzyme family (Curran et al 2004;Chen KE et al 2018;Qiao et al 2018) and are used to disrupt the interaction of cells-cells and cells-ECM by tissue remodelling. Studies have shown that the MMPs, particularly MMP-2 and MMP-9, key members of the MMP family, play a vital role in angiogenesis, invasion and transferring of tumours, including RCC (Ramos et al 2016;Chen et al 2017;Beardo et al 2019). Therefore, gelatine zymography was performed to determine the activity of MMP-2/-9.…”

Section: Discussionmentioning

confidence: 99%

Resveratrol inhibits ACHN cells via regulation of histone acetylation

Dai

Chen

Wang

et al. 2020

Pharmaceutical Biology

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Context: The relationship between resveratrol and histone acetylation in renal cell carcinoma (RCC) has not yet been reported. Objective: To explore the functional role of resveratrol in RCC. Materials and methods: Functional experiments were performed to determine proliferatio n of ACHN cells with treatment of resveratrol (0, 7.8125, 15.625, 31.25 and 62.5 lg/mL, for 12, 24 and 48 h of culture) or 0.1 lM SAHA. The enzyme activities of MMP-2/-9 were measured by gelatine zymography and histone acetylation by Western blot. Results: When the cells were treated with 15.625, 31.25 and 62.5 lg/mL resveratrol, ACHN cells viability was 73.2 ± 3.5%, 61.4 ± 3.1%, 50.2 ± 4.7% for 12 h, 62.7 ± 4.5%, 52.4 ± 5.5%, 40.2 ± 3.8% for 24 h, and 60.8 ± 3.7%, 39.4 ± 5.1%, 37.6 ± 2.7% for 48 h, and the wound closure (%) of migration was increased from 0.6 to 0.7, 0.85, 0.9 for 12 h and from 0.23 to 0.3, 0.48, 0.59 for 24 h. The invasion rate was 8.5 ± 0.9%, 7.4 ± 0.3% and 5.8 ± 0.6%, and cell cycle was arrested at G1 from 42.5 ± 2.9% to 55.3 ± 5.7%, 59.8 ± 3.4%, 68.7 ± 4.6%. MMP-2/-9 expression (p < 0.05) was inhibited by resveratrol. The protein levels of histone acetylation (p < 0.01) was increased by resveratrol. Discussion and conclusions: Our results suggest that these effects might be related to a high level of histone acetylation, and resveratrol can be considered as an alternative treatment for RCC.

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“…MMP-2 levels in circulating blood have been considered as a breast cancer metastasis marker and are related to mortality rates (19,20). 90% of patients that had worse prognoses (metastases and mortality), in a Brazilian cohort, had positive staining for MMP-2 in their breast cancer tumors (21), indicating they were in a "high-risk group". Clinical and preclinical studies have demonstrated MMP-2 involvement in several human cancers, including prostate, gastric, and pancreatic cancers.…”

Section: Role Of Evps Cargo As Potential Mechanisms For the Beneficia...mentioning

confidence: 99%

Potential involvement of circulating extracellular vesicles and particles on exercise effects in malignancies

et al. 2023

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Physical activity and exercise have been widely related to prevention, treatment, and control for several non-communicable diseases. In this context, there are innumerous pre-clinical and clinical evidence indicating the potential role of exercise, beyond cancer prevention and survival, improved quality of life, including on psychological components, bone health and cachexia, from cancer survivors is described as well. This mini-review raises the potential role of circulating extracellular and particles vesicles (EVPs) cargo, as exerkines, conducting several positive effects on adjacent and/or distant tissues such as tumor, immune, bone and muscle cells. We highlighted new perspectives about microRNAs into EVPs changes induced by exercise and its benefits on malignancies, since microRNAs can be implicated with intricated physiopathological processes. Potential microRNAs into EVPs were pointed out here as players spreading beneficial effects of exercise, such as miR-150-5p, miR-124, miR-486, and miRNA-320a, which have previous findings on involvement with clinical outcomes and as well as tumor microenvironment, regulating intercellular communication and tumor growth. For example, high-intensity interval aerobic exercise program seems to increase miR‐150 contents in circulating EVPs obtained from women with normal weight or overweight. In accordance circulating EVPs miR-150-5p content is correlated with prognosis colorectal cancer, and ectopic expression of miR-150 may reduce cell proliferation, invasion and metastasis. Beyond the involvement of bioactive miRNAs into circulating EVPs and their pathways related to clinical and preclinical findings, this mini review intends to support further studies on EVPs cargo and exercise effects in oncology.

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Worse prognosis in breast cancer patients can be predicted by immunohistochemical analysis of positive MMP-2 and negative estrogen and progesterone receptors

Cited by 8 publications

References 23 publications

Overexpression of collagen type V α1 chain in human breast invasive ductal carcinoma is mediated by TGF-β1

Overexpression of collagen type V α1 chain in human breast invasive ductal carcinoma is mediated by TGF-β1

Resveratrol inhibits ACHN cells via regulation of histone acetylation

Potential involvement of circulating extracellular vesicles and particles on exercise effects in malignancies

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