The effects of fat graft and platelet-rich fibrin combination after epineurectomy in rats

Kastamoni, Menekşe; YAVAŞ, Senem Esin; Özgenel, Güzin Yeşim; ERSOY, Semiha

doi:10.1590/1806-9282.20220982

Cited by 3 publications

(1 citation statement)

References 17 publications

(26 reference statements)

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“…The scientific literature indicates the efficiency of immunofluorescence methods for analyzing the regeneration of Schwann cells and peripheral remyelination [61,62]. In agreement, Kastamoni et al (2023) [32] found that the expression of EphA4 was associated with a negative effect on the axonal repair of the sciatic nerve, consistent with previous studies [63][64][65].…”

Section: Histological Analysissupporting

confidence: 82%

Evaluation of Postoperative Nerve Regeneration in Murine Experimental Models Subjected to Nerve Transection

Araújo,

Mussalem,

Bani

et al. 2024

Preprint

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Objectives: This study aims to explore the use of murine models in peripheral nerve transection research, evaluating and synthesizing key methods for analyzing nerve regeneration to guide future research and clinical interventions. Methodology: A systematic review was conducted in February 2024, adhering to Cochrane and PRISMA 2020 guidelines, using Medline, Scielo, and Lilacs databases. The focus was on experimental studies of nerve regeneration in animal models post-transection. Only experimental clinical trials reporting nerve regeneration outcomes were included; studies lacking a comparator group or functional evaluation methods were excluded. Results: Out of 273 studies initially identified from MEDLINE, 19 were selected for detailed analysis. The average study included 32.5 subjects, with about 10.16 subjects per intervention subgroup. The predominant model was the sciatic nerve injury with a 10mm gap. The most common intervention involved unprocessed adipose-derived stem cells, utilized in 14 articles. Conclusions: The review underscores the significant potential of current methodologies in peripheral nerve regeneration, particularly highlighting the use of murine models and thorough evaluation techniques. These studies significantly contribute to our understanding of nerve regeneration processes and inform directions for future research.

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Section: Histological Analysissupporting

confidence: 82%

Evaluation of Postoperative Nerve Regeneration in Murine Experimental Models Subjected to Nerve Transection

Araújo,

Mussalem,

Bani

et al. 2024

Preprint

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Adipose Tissue Derivatives in Peripheral Nerve Regeneration after Transection: A Systematic Review

Araújo,

Mussalem,

Carrijo

et al. 2024

Bioengineering

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Introduction: Peripheral nerve injury (PNI) is increasingly prevalent and challenging to treat despite advances in microsurgical techniques. In this context, adipose tissue derivatives, such as adipose-derived stem cells, nanofat, and stromal vascular fraction have been gaining attention as potential allies in peripheral nerve regeneration. Objectives: This study aims to explore the use of adipose tissue derivatives in nerve regeneration following peripheral nerve transection in murine models. Thus, we assess and synthesize the key techniques and methods used for evaluating the obtained nerve regeneration to guide future experimental research and clinical interventions. Methodology: A systematic review was conducted in February 2024, adhering to the Cochrane and PRISMA 2020 guidelines, using the PubMed, SciELO, and LILACS databases. The focus was on experimental studies involving adipose tissue derivatives in nerve regeneration in animal models post-transection. Only experimental trials reporting nerve regeneration outcomes were included; studies lacking a comparator group or evaluation methods were excluded. Results: Out of 273 studies initially identified from MEDLINE, 19 were selected for detailed analysis. The average study included 32.5 subjects, with about 10.2 subjects per intervention subgroup. The predominant model was the sciatic nerve injury with a 10 mm gap. The most common intervention involved unprocessed adipose-derived stem cells, utilized in 14 articles. Conclusions: This review underscores the significant potential of current methodologies in peripheral nerve regeneration, particularly highlighting the use of murine models and thorough evaluation techniques.

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Identification of Mast Cells and Immunophenotypic Subtypes in Peripheral Nerve Damage Caused by Epineurotomy and Systemic Inflammation

Yavas,

Kavruk

et al. 2023

Neurological Sciences and Neurophysiology

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Purpose: This study aims to investigate the numerical increase, localization, granulation status, and immunophenotypic properties of mast cells (MCs) in epineurectomy-induced nerve damage and lipopolysaccharide (LPS)-induced systemic infection models. Materials and Methods: In this study, the animals were divided into three groups of 6 each. One of the groups was determined as the control group, epineurectomy was applied to one group, and systemic inflammation was created by regular LPS injections in the other group. Then, the obtained nerve tissues were stained histochemically with Hematoxylin and Eosin toluidine blue, and the increase, localization, and granulation status of MCs were examined. Immunohistochemically, antitryptase and antichymase staining were performed to determine the immunophenotypes of MCs. Results: As a result, while the number of MCs increased in both groups compared to the control group, MCs in the LPS group were in the epineurium, and MCs in the epineurotomy group were located between the nerve fibers. While MCs in the LPS group showed very severe degranulation, mild degranulation was observed in the epineurotomy group, and almost no degranulated MCs were observed in the control group. Conclusion: This study is critical because it is one of the first studies to compare MCs in different nerve damage types and examine the expression of chymase and tryptase.

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The effects of fat graft and platelet-rich fibrin combination after epineurectomy in rats

Cited by 3 publications

References 17 publications

Evaluation of Postoperative Nerve Regeneration in Murine Experimental Models Subjected to Nerve Transection

Evaluation of Postoperative Nerve Regeneration in Murine Experimental Models Subjected to Nerve Transection

Adipose Tissue Derivatives in Peripheral Nerve Regeneration after Transection: A Systematic Review

Identification of Mast Cells and Immunophenotypic Subtypes in Peripheral Nerve Damage Caused by Epineurotomy and Systemic Inflammation

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