Evaluation of the rs35996865 polymorphism of the ROCK1 gene in sepsis

Abstract

OBJECTIVE: Sepsis is a complex and serious medical condition resulting from the activation of an innate host response to infections. The etiology of sepsis is complex and can be influenced by genetic susceptibility. The purpose of the present study was to investigate a possible association of Rhokinase 1 (ROCK1) gene polymorphism with sepsis in a Turkish population. METHODS:The study group consisted of 100 unrelated patients with sepsis and 100 healthy controls. Genomic DNA was isolated from peripheral leukocytes from EDTA-containing blood using the QIAamp DNA Blood Mini Kit. ROCK1 gene rs35996865 and rs112130712 (Lys1054Arg) polymorphisms were analyzed in genomic DNA using the LightCycler 480 II real-time polymerase chain reaction. RESULTS: There were no significant differences in allele and genotype frequencies for ROCK1 gene rs35996865 polymorphism between the patients with sepsis and control group (p>0.05). Additionally, no association was detected between the rs35996865 polymorphism and mortality in the patient group. No polymorphism was detected with ROCK1 gene rs112130712 (Lys1054Arg) in our study groups. CONCLUSIONS: Our data showed that there is no marked association between the rs35996865 polymorphism and sepsis. Therefore, these results suggest that ROCK1 gene rs35996865 polymorphism is not risk factor for the development of sepsis in the Turkish population.

Evaluation of the Rho-kinase gene expression and polymorphisms in adult patients with acute appendicitis: a differential impact of gender

“…However, it is not known whether this polymorphism is able to alter the expression level of the ROCK1 gene 6 . The ROCK1 gene rs35996865 variant mapping to the 5ʹ-UTR has been reported to be markedly associated with obesity-related metabolic syndrome 7 , respiratory distress syndrome 8 , and nonsyndromic cleft palate 6 , but not with sepsis 9 or primary open-angle glaucoma 10 . The result of our study showed that there was no association between the rs35996865 variant and AA.…”