Effect of Lactobacillus species on apoptosis-related genes BCL2, BAX, and caspase 3 in the testes of gamma-irradiated rats

Changizi, Vahid; Azadbakht, Omid; Ghanavati, Roya; Behrouj, Hamid; Motevaseli, Elahe; Khanzadeh, Pegah

doi:10.1590/1806-9282.20210634

Cited by 11 publications

(5 citation statements)

References 25 publications

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“…A high-fat diet induces intestinal flora disorders, resulting in testicular dysfunction and decreased spermatogenesis (Ding et al, 2020 ). In addition to this, Lactobacillus species significantly reversed testicular damage induced by gamma irradiation in rats (Changizi et al, 2021 ). Lactobacillus casei and Lactobacillus coagulans supplementation have protective effects against CCl 4 -induced testicular toxicity, thereby increasing the rate of spermatogenesis in rats (Keshtmand et al, 2021 ).…”

Section: Discussionmentioning

confidence: 98%

The Improvement of Semen Quality by Dietary Fiber Intake Is Positively Related With Gut Microbiota and SCFA in a Boar Model

et al. 2022

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Although fiber-rich diets have been positively associated with sperm quality, there have not been any studies that have examined the effects of dietary fiber and its metabolites on sperm quality in young or pre-pubescent animals. In this study, we aimed to explore the effect of dietary fiber supplementation on semen quality and the underlying mechanisms in a boar model. Sixty purebred Yorkshire weaning boars were randomly divided into the four groups (T1–T4). Groups T1, T2, and T3 boars were fed diets with different levels of fiber until reaching 160 days of age and were then fed the same diet, while group T4 boars were fed a basal diet supplemented with butyrate and probiotics. Compared with T1 boars, sperm motility and effective sperm number were significantly higher among T3 boars. Meanwhile, at 240 days of age, the acetic acid and total short-chain fatty acid (SCFA) contents in the sera of T3 and T4 boars were significantly higher than those in T1 boars. The abundance of microbiota in T2 and T3 boars was significantly higher than that in T1 boars (P < 0.01). Moreover, dietary fiber supplementation increased “beneficial gut microbes” such as UCG-005, Rumenococcus, Rikenellaceae_RC9_gut_group and Lactobacillus and decreased the relative abundance of “harmful microbes” such as Clostridium_sensu_stricto_1, Romboutsia and Turicibacter. Collectively, the findings of this study indicate that dietary fiber supplementation improves gut microbiota and promotes SCFA production, thereby enhancing spermatogenesis and semen quality. Moreover, the effects of dietary fiber are superior to those of derived metabolites.

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Section: Discussionmentioning

confidence: 98%

The Improvement of Semen Quality by Dietary Fiber Intake Is Positively Related With Gut Microbiota and SCFA in a Boar Model

et al. 2022

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“…Similarly, this study revealed that Gamma-radiation inhibited Bcl-2 and activated BAX and caspase-3 genes in healthy animal tissues, and Lactiplantibacillus spp. treatment was able to reverse such effects and acted as natural radioprotectors [ 44 ]. On the other hand, LP 06CC2 extract significantly suppressed cell viability of Caco2 colorectal cancer cells by inducing apoptosis through the JNK/p38 MAPK signaling system [ 45 ].…”

Section: Discussionmentioning

confidence: 99%

Lactiplantibacillus plantarum Induces Apoptosis in Melanoma and Breast Cancer Cells

Budu,

Mioc,

Soica

et al. 2024

Microorganisms

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Despite the notable advancements witnessed in the past decade in medical and health research domain, cancer remains a prominent global cause of mortality. Moreover, the conventional treatments employed to combat this disease have been found to considerably compromise the quality of life experienced by patients due to its severe side effects. Recent in vitro studies revealed encouraging findings on the potential beneficial effects of probiotics as adjuvants of anticancer therapy, and even as possible agents for the prevention and treatment of various types of malignancies. From this standpoint, the primary objective of this work was to investigate the anticancer properties of Lactiplantibacillus plantarum (LP) and elucidate its underlying mechanism of action. In order to investigate this matter, several doses of LP (ranging from 105 to 1010 CFU/mL) were examined in relation to melanoma cancer cell lines (A375) and breast cancer cell line (MCF-7). The cell viability findings, which were substantiated by morphological investigations and annexin V/PI assay, indicated that LP exerted inhibitory effects on cellular activity and triggered apoptosis. Additionally, upon further investigation into its mechanism, it was observed through the apoptosis assay and Western blot analysis that the administration of LP resulted in an elevation of pro-apoptotic BAX protein levels and an upregulation of cleaved poly-ADP-ribose polymerase (PARP) protein expression. Conversely, the levels of anti-apoptotic Bcl-2 protein were found to decrease in the A375 and MCF-7 cell lines. These findings provide insight into the pro-apoptotic mechanism of action of LP in these specific cell lines.

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“…Downregulation of Bcl‐2 and upregulation of Bax gene expression can both promote apoptosis. [ 21,35 ] Inhibition of the expression level of Bcl‐2 may induce the activation of p38, which promotes cell apoptosis. [ 36 ] An imbalance of Bcl‐2 and Bax proteins might result in the release of cytochrome C, which triggers caspase 3 and PARP activation and leads to apoptosis.…”

Section: Discussionmentioning

confidence: 99%

Fraxetin inhibits proliferation and induces apoptosis of bladder cancer through the Akt pathway in vitro and in vivo

Weng,

Deng,

Huang

et al. 2023

J Biochem & Molecular Tox

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Fraxetin, a natural compound extracted from the Chinese herb Cortex Fraxini, is reported to boast extensive antitumor properties in various cancers. However, whether fraxetin exhibited an anticancer effect on bladder cancer remains unknown. In this study, cell counting kit‐8 was utilized to detect cell viability. Flow cytometry analysis was performed for cell apoptosis analysis. Western blot analysis and real‐time PCR were used to ascertain gene expression analysis. A mouse bladder cancer xenograft model was established and subjected to fraxetin treatment. Fraxetin reduced the viability of bladder cancer cells, induced apoptosis in vitro, and inhibited the growth of bladder cancer in vivo. Fraxetin inhibited the Akt pathway in J82 cells. In conclusion, the growth inhibitory properties of fraxetin against bladder cancer may be mediated via an Akt inhibitory effect and cell apoptosis promotion.

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Effect of Lactobacillus species on apoptosis-related genes BCL2, BAX, and caspase 3 in the testes of gamma-irradiated rats

Cited by 11 publications

References 25 publications

The Improvement of Semen Quality by Dietary Fiber Intake Is Positively Related With Gut Microbiota and SCFA in a Boar Model

The Improvement of Semen Quality by Dietary Fiber Intake Is Positively Related With Gut Microbiota and SCFA in a Boar Model

Lactiplantibacillus plantarum Induces Apoptosis in Melanoma and Breast Cancer Cells

Fraxetin inhibits proliferation and induces apoptosis of bladder cancer through the Akt pathway in vitro and in vivo

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