Exploring the expression profile of vitamin D receptor and its related long non-coding RNAs in patients with acute lymphoblastic leukemia

Abstract

OBJECTIVE: Acute lymphoblastic leukemia (ALL) is the most common type of childhood cancer. Previous studies have indicated the involvement of vitamin D receptor (VDR) and related long noncoding RNAs (lncRNAs) signaling in the pathophysiology of several cancers. However, their contribution to ALL remains to be elucidated. METHODS:In this case-control study, 30 patients with newly diagnosed ALL and 30 age-and sex-matched healthy children were selected.Then, the level of 25(OH) vitamin D and the expression of VDR and four VDR-related lncRNAs were assessed. RESULTS:No significant difference in serum 25(OH) vitamin D was observed between patients with ALL (20.42±6.5 ng/mL) and healthy subjects (25.45±11 ng/mL). In addition, the expression of MALAT-1, HOTAIR, and P-21 was not statistically significant between the two groups. However, a significant reduction in VDR and H19 expression was observed in patients with ALL (p<0.05).CONCLUSIONS: 25(OH) vitamin D insufficiency was evident in both groups. VDR and H19 signaling might be contributed to the pathogenesis of ALL, which needs further investigations.

Expression analysis of vitamin D receptor-associated long noncoding RNAs in patients with relapsing-remitting multiple sclerosis

“…Then PBMCs were isolated using Ficoll density gradient centrifugation. After washing the PBMCs twice with phosphate-buffered saline solution, total RNA was extracted using Trizol (GeneAll, South Korea) (18). Subsequently, cDNA was synthesized from the extracted total RNAs using the cDNA synthesis kit (Parstous, Iran).…”

“…All reactions were carried out in duplicate using the Mic PCR system (BMS, Australia). The temperature profi le of PCR reactions was as follows: 95 °C for 15 min, followed by 40 cycles including 95 °C for 40 s, 62 °C for 25 s, and 72 °C for 15 s (18,19).…”

Exploring the nexus of nuclear receptors in hematological malignancies

“…For instance, the ER shows elevated expression in oral cancer, while its expression varies in lung cancer [ 50 , 51 ]. Similarly, VDR exhibits diminished expression in HM but presents differential expression in esophageal cancer [ 38 , 59 , 60 ]. Additionally, distinct isotypes of specific NRs, like PPAR, have unique functional implications.…”

“…The intracellular receptor, VDR, upon binding with its active metabolite 1,25(OH)2D, becomes the focal point for the transcriptional modulation of numerous target genes [ 229 , 232 ]. Multiple studies have reported the downregulation of VDR expression in HM [ 59 , 60 ].…”