SYMBIOTIC EFFICIENCY OF INOCULATION WITH NITROGEN-FIXING BACTERIA AND ARBUSCULAR MYCORRHIZAL FUNGI IN Tachigali vulgaris SEEDLINGS

Abstract

The present study aimed to evaluate the effect of inoculation with nitrogen-fixing bacteria (NFB) and arbuscular mycorrhizal fungi (AMF) on the development of Tachigali vulgaris seedlings under nursery conditions. The seedlings were produced in 1 kg bags on a substrate of sand and vermiculite (1:1), following a completely randomized experimental design in a 3 (NFB) x 2 (with and without AMF) factorial scheme with 3 additional control treatments consisting of: seedlings inoculated only with AMF (mycorrhizal control), non-inoculated seedlings fertilized with N (nitrogenized control) and without N (absolute control). The following variables were evaluated: height, stem diameter (SD), shoot dry mass (SDM), root dry mass (RDM) and nodule dry mass (NDM), P accumulation in the shoot, and root mycorrhizal colonization (RMC). Efficiency and effectiveness were calculated to evaluate the plant response to double inoculation. The treatments showed a significant effect for all variables, except for mycorrhizal colonization, nodule number, and dry mass, with the nitrogen treatment having the highest growth values. Plants submitted to double inoculation showed a higher accumulation of dry matter, height and SD, reaching a 124% higher RDM regarding the absolute control, 90% more SDM, and 207% more NDM regarding the seedlings inoculated only with rhizobia. The positive effect of double inoculation occurred regardless of the strain used. The results indicate that the joint inoculation of NFB and AMF was beneficial for the species, promoting its growth.

Potencial Simbiótico de Microrganismos do Solo na Promoção do Crescimento de Espécies Arbóreas da Mata Atlântica

“…A possible explanation is the amount of phosphorus present in the nutrient solution (3.2 mg of P per plant every two weeks). In general, plants show less responsiveness to mycorrhization in soils with high levels of P (Pedone-Bonfim et al 2018), as showed by Freire et al (2020) in the mycorrhizal colonization of the forest tree species Tachigali vulgaris L.F. Gomes da Silva & H.C. Lima when using the same nutrient solution as in the current research, both under greenhouse conditions. In another study, Silva et al (2016) showed a decrease in mycorrhizal colonization in Cecropia pachystachya Trécul with increasing P levels in the soil, but without affecting the height, stem diameter, biomass, and phosphorus levels in the aboveground parts of the plants.…”

“…Several other studies show how the synergistic effect of tripartite association can maximize nutrient acquisition, thus favoring the development and growth of tree legumes (Gross et al 2004;Borges et al 2016;Oliveira Júnior et al 2017;Bournaud et al 2018;Silva 2018;Afkhami et al 2020;Freire et al 2020;Silva 2022). For example, Gross et al (2004) found significantly higher nodulation in A. peregrina plants when inoculated with both NFB and AMF, compared to the treatment containing only NFB.…”

Estabelecimento de Anadenanthera colubrina var. cebil sob inoculação micorrízica e fertilização no Pantanal brasileiro

“…cebil sob inoculação micorrízica e fertilização no Pantanal brasileiro A. colubrina produzidas com inoculação micorrízica e sem fertilização química, em plantio estabelecido no bioma Mata Atlântica. Neste cenário, estudos recentes apontam como alternativa ao manejo microbiológico de espécies arbóreas a dupla inoculação envolvendo fungos micorrízicos e rizóbios (Marques et al, 2001;Gross et al, 2004;Dias et al, 2012;Cely et al, 2016;Tavares et al, 2016;Bournaud et al, 2018, Freire et al, 2020, não avaliada no presente estudo. Para as duas condições de inoculação, informações adicionais sobre a compatibilidade entre inóculos e hospedeiro (Gross et al, 2004), a competitividade dos materiais selecionados com microorganismos nativos do solo (Chu et al, 2001) e a prospecção de organismos indígenas simbiontes (Santos et al, 2008a;Barea et al, 2011) poderão subsidiar, no futuro, a adequação da inoculação às especificidades de cada contexto em que se aplica a recomposição vegetal (Santos et al, 2008b).…”

Soil of the parent plant and AMF mix improve Cerrado’s seedlings growth in forest nurseries

“…The aforementioned AMF species are frequently found in the Cerrado, which shows a high diversity of AMF (Pagano & Scotti 2010, Fernandes et al 2016, Pontes et al 2017. Previous studies reported satisfactory results in the growth of native Cerrado seedlings using the aforementioned AMF species (Abreu et al 2018, Freire et al 2020. Furthermore, the results of those studies indicated that H. speciosa and B. gaudichaudii can also benefit from the symbiotic association with AMF.…”