Impact of Phytobiotic Growth Promoters Supplementation on the Morphology and Biometry of the Vital Organs in Broilers

Abstract

Supplementation of Cv (clove) and Ts (tulsi) augments the growth rate and gut health of broilers. However, studies on their impact on the vital organs and male reproductive system remain scarce. Therefore, the current study aimed to investigate the impact of Cv and Ts supplementation on hematological profiles, as well as the heart, lungs, liver, kidney, and testes of broilers, both morphologically and biometrically. Sixty one-day-old broiler chicks, divided into four homogenous groups (control-T0 and three supplement groups-T1, T2, and T3), were fed 0.5% Cv and 2% Ts (T1), 1% Cv and 3% Ts (T2), and 1.5% Cv and 4% Ts (T3) with drinking water for 21 d. Organ samples were collected on d 14 and d 28 for macroscopic and microscopic (hematoxylin and eosin stain) investigation. Cv and Ts didn't affect the hematological profile, organ weight gain, and their general histoarchitectures. However, the histomorphometric investigation showed an increased (p<0.05) parabronchus surface area of the lungs, size of the renal glomeruli, the diameter of the proximal and distal convoluted tubules of the kidney in the T1 and T2 groups, and size of the seminiferous tubules of the testes in group T1. These findings suggest that unlike antibiotic or steroid growth promoters (GPs), Cv and Ts have beneficial impacts on the vital organs as well as on the male gonad of broilers from the morphologic and biometric perspectives.