Expression of TGF-β/Smads in Cecum and Spleen of Chicken Infected with E. Tenella

Huang, Jun; Yin, Huaizhi; Zhang, Y; Qiao, Hanzhen; Su, Lihe; Wang, Jinrong

doi:10.1590/1806-9061-2021-1446

Cited by 4 publications

(1 citation statement)

References 26 publications

(31 reference statements)

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“…Most species of the genus Eimeria have different sites of infection. Eimeria necatrix and E. tenella are considered to be the most pathogenic species in chickens and infect the small intestine and cecum, respectively ( 10 , 11 ). Mixed infections by different species can result in more severe presentations of the disease, as most species can affect different parts of the intestine ( 9 , 12 ).…”

Section: Introductionmentioning

confidence: 99%

Prevalence and diversity of Eimeria spp. in free-range chickens in northeastern Brazil

et al. 2022

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In tropical regions, family farming is a form of production and work that is highly present in rural areas. Because the production system for free-range chickens has a low level of technification, it frequently presents massive infection by coccidia. The objective of this study was to determine the prevalence and diversity of Eimeria species in free-range chickens in northeastern Brazil. Fecal analyses were carried out using materials collected from 100 farms, belonging to 10 different municipalities. The sample from each farm was composed of five stool samples collected from different animals. Coproparasitological analyses were performed and, in each positive sample, photomicrographs of 20 oocysts were used for morphological identification of coccidia. The presence of Eimeria spp. was detected in 59% (59/100) of the farms analyzed. Species identification was performed through morphometric analysis of 1,180 sporulated oocysts. The following eight species of Eimeria spp. were found, in decreasing order of prevalence: Eimeria necatrix (25%), Eimeria mitis (18.3%), Eimeria mivati (17.3%), Eimeria tenella (12.4%), Eimeria brunetti (9.9%), Eimeria acervulina (9.1%), Eimeria praecox (4.8%) and Eimeria maxima (3.2%). The prevalence and diversity of Eimeria spp. on farms producing backyard chickens in the semiarid region of Brazil were high, especially the diversity of species. Changing the management, with the adoption of sanitary measures, may be effective in reducing the high prevalence of coccidia on the farms studied.

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Section: Introductionmentioning

confidence: 99%

Prevalence and diversity of Eimeria spp. in free-range chickens in northeastern Brazil

et al. 2022

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Prophylactic and Therapeutic Efficacy of Ultrasonicated Rosmarinus officinalis Ethanolic Extract and its Chitosan-Loaded Nanoparticles Against Eimeria tenella Infected Broiler Chickens

Kasem,

Mira,

Helal

et al. 2024

Acta Parasit.

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Purpose The in vivo efficacy of ultrasonicated Rosmarinus officinalis ethanolic extract (UROEE) and its chitosan-loaded nanoparticles (UROEE-CsNPs) was investigated as a dietary prophylactic agent and as a therapeutic treatment against Eimeria tenella infected broiler chickens. Methods Chickens were infected with 4 × 104E. tenella oocysts at 21 days old for primary infection and with 8 × 104 oocysts at 35 days old for secondary infection. Eleven experimental groups were conducted. Dietary addition of 100 mg/kg UROEE and 20 mg/kg for CsNPs as well as UROEE-CsNPs were included for prophylactic groups from day 1 to 42. The same doses were used for therapeutic treatment groups for 5 constitutive days. Oocyst output in feces was counted. Histopathological and immunohistochemical studies were conducted. Gene expression of pro-inflammatory cytokines as IFN-γ, IL-1β and IL-6 as well as anti-inflammatory cytokines as IL-10 and TGF-β4 was analyzed using semi-quantitative reverse transcriptase-PCR. Results The results showed an efficacy of UROEE, CsNPs and UROEE-CsNPs in reduction of oocyst excretion and improving the cecal tissue architecture. CD4+ and CD8+ T lymphocytes protein expression were reduced. E. tenella infection lead to upregulation of pro-inflammatory cytokines as IFN-γ, IL-1β, IL-6 and anti-inflammatory cytokines as TGF-β4 following primary infection, while their expression was downregulated following secondary infection. Conclusion The dietary prophylactic additives and therapeutic treatments with UROEE, CsNPs and UROEE-CsNPs could decrease the inflammatory response to E. tenella as indicated by oocyst output reduction, histopathological improvements, CD4+ and CD8+ T cells protein expression reduction as well as reducing mRNA expression levels of the tested cytokines following primary and secondary infections. Consequently, these results will help to develop better-combating strategies for the control and prevention of coccidiosis on poultry farms as a dietary prophylactic agent or as a therapeutic treatment. Graphical Abstract

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Identification of the Hub Genes Linked to Lead (IV)-Induced Spleen Toxicity Using the Rat Model

Yang,

Wang,

et al. 2023

Biol Trace Elem Res

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Expression of TGF-β/Smads in Cecum and Spleen of Chicken Infected with E. Tenella

Cited by 4 publications

References 26 publications

Prevalence and diversity of Eimeria spp. in free-range chickens in northeastern Brazil

Prevalence and diversity of Eimeria spp. in free-range chickens in northeastern Brazil

Prophylactic and Therapeutic Efficacy of Ultrasonicated Rosmarinus officinalis Ethanolic Extract and its Chitosan-Loaded Nanoparticles Against Eimeria tenella Infected Broiler Chickens

Identification of the Hub Genes Linked to Lead (IV)-Induced Spleen Toxicity Using the Rat Model

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