Effects of β-mannanase on Egg Production Performance, Egg Quality, Intestinal Microbiota, Viscosity, and Ammonia Concentration in Laying Hens

Zheng, Lan; Cho, Seung-Hun; Kang, CW; Lee, Kyung-Woo; Kim, Kwan Eung; An, Byoung-Ki

doi:10.1590/1806-9061-2019-1180

Cited by 6 publications

(9 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Based on our in vitro data in this paper, and published in vivo data (Kong et al, 2011;Mussini et al, 2011, Zheng et al, 2020 we can confirm the two modes of action of CTCzyme as an NSPase: (1) Solubilization of mannan in the cells walls to loosen and destroy the compact cell wall architecture thereby making the protein more bioavailable, and (2) Release of mannose oligomers considered to have prebiotic functions for improving gut health and integrity.…”

Section: Oligomsupporting

confidence: 67%

“…In the same way, using suboptimal nutrient levels in a broiler trial Ferreira et al (2016) showed an improved AMEn of 1.5% with the inclusion of CTCzyme. With supplementation of CTCzyme β-mannanase in a low-energy and low-protein diet, laying hens were able to maintain similar production performance when compared to a high-energy and high-protein diet during early and late egg production (Zheng et al, 2020). There was no inclusion of any low molecular weight fermentable oligosaccharides in the above mentioned in vivo work besides the enzyme as opposed to the work of Cordero et al (2019) which included both enzyme and fermentable oligosaccharides (in the experiment: a xylanase and xylan oligosaccharides).…”

Section: Oligommentioning

confidence: 99%

See 1 more Smart Citation

Confirmation of Cage Effect and Prebiotic Production Potential of a β-Mannanase, with SBM as Substrate Using Microscopy and Wet Chemistry

Gomez-Osorio¹,

Oh²,

Lee³

2021

JAS

View full text Add to dashboard Cite

In vitro assays were carried out to investigate the solubilization of cell walls and generation of mannan oligosaccharides of a b-mannanase-containing commercial product on SBM. Using commercial dosages of the b-mannanase (500 g per ton of feed) cell wall degradation of mannan in SBM cell walls was visualized and an increase in reducing ends (0.12±0.02 mg/mL) and the generation of mannan oligosaccharides of degree of polymerization 2 and 4 (22.9±3.2 mg/L and 398.8±25.4 mg/L) were also measured using HPLC. Mannan, which is H-bonded to cellulose and xyloglucan, was solubilized using a single monocomponent enzyme, allowing for visualization of the disintegration of the entire SBM cell wall structure. This work is the first of its kind using strictly commercial dosage levels of enzyme for evaluating efficacy of the same microscopically. These data confirm the hypothesis that there most likely is a need for only a single relevant NSP enzyme targeting its specific substrate, independent of the concentration of the latter within the complex polysaccharide matrix in the plant cell wall to experience the beneficial effects of the enzyme both in vitro and in vivo. An analogy to compare our data would be destruction of the foundation (mannan) of a building or a bridge (soybean cell wall) which would inevitably lead to dismantling or demolition the entire building or bridge.

show abstract

Section: Oligomsupporting

confidence: 67%

Section: Oligommentioning

confidence: 99%

Confirmation of Cage Effect and Prebiotic Production Potential of a β-Mannanase, with SBM as Substrate Using Microscopy and Wet Chemistry

Gomez-Osorio¹,

Oh²,

Lee³

2021

JAS

View full text Add to dashboard Cite

show abstract

“…However, hens receiving a low AME diet with 0.04% β-mannanase had thicker eggshells than hens fed other diets. Supplemental β-mannanase (800,000 U/kg) had no effects on egg quality measures (shell color, thickness, strength, yolk color, and Haugh unit) during early and late lay in Hy-line brown hens ( Zheng et al, 2020 ).…”

Section: Introductionmentioning

confidence: 97%

“…However, the hens fed β-mannanase had higher egg production, egg mass, and retained more gross energy and crude protein. Supplemental β-mannanase (800,000 U/kg) in lower AME and crude protein diets resulted in comparable egg production and egg mass to high AME and crude protein diets during early (30 weeks) and late (>62 weeks) laying cycle in Hy-Line brown laying hens ( Zheng et al, 2020 ). The effect of dietary β-mannans (1.05% vs. 2.33%) and supplemental β-mannanase (0%, 0.04%, and 0.08%) was investigated for 8 weeks in 52-wk-old Hy-line brown hens ( Ryu et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Significance of single β-mannanase supplementation on performance and energy utilization in broiler chickens, laying hens, turkeys, sows, and nursery-finish pigs: a meta-analysis and systematic review

Kiarie

Steelman

Martinez

2021

Translational Animal Science

View full text Add to dashboard Cite

This review will give a brief description of β-mannans, abundance in feedstuffs, utility of supplemental feed β-mannanase and subsequent animal responses. Soybean products and co-products of processing palm, coconut and guar seeds are the major sources of β-mannans in poultry and livestock feed. β-mannans are linear polymers of mannose residues linked by β-1,4 glycosidic bonds and their ingestion elicit undesirable and metabolically costly responses. Web of Science was searched to retrieve published studies for meta-analyses of the impact of supplemental β-mannanase on performance and digestibility in pigs and poultry. The mean difference (MD) between ß-mannanase and control on average daily gain (ADG, g/d) was +0.23 (P=0.013; 95% CI 0.05; 0.41), +10.8 g/d (P=0.0005; 95% CI of 6.6; 15.0 g/day) and +20.68 (P<0.000; 95% CI of 17.15; 24.20 g/day) for broiler chickens, nursery pigs and grow-finish pigs, respectively. The MD on β-mannanase improvement on FCR was -0.02 (P<0.0001) with 95% CI (-0.03; -0.02) suggesting a 2-to-3-point FCR improvement in broiler chickens. ß-mannanase improvement on gain to feed (G:F) was +13.8 g/kg (P=0.027; 2.1; 25.4 g/kg) and +8.77 g/kg (6.32; 11.23 g/kg) in nursery and grow-finish pigs, respectively. β-mannanase improved AMEn by 47 kcal/kg (P=0.0004) with 95% CI (28.8; 65.7 kcal/kg) in broiler chickens. The improvement of gross energy digestibility in pigs was 1.08 percentage unit with 95% CI (0.90; 1.26) translating to the release of between 30.6 and 42.8 kcal/kg of digestible energy. Although data was limited, β-mannanase improved egg production but had no impact on egg quality in laying hens. Turkeys may be more adversely affected by ß-mannans because of the high protein/ amino acids requirements necessitating higher dietary inclusion of soybean meal. However, growth performance and feed efficiency responses of turkeys fed diets supplemented with β-mannanase was variable. In summary, ß-mannanase supplementation improved performance linked to energy and nutrient utilization. However, the magnitude of response was variable within and between species indicating further application refinement is warranted to achieve consistent efficacy, including an improved understanding of the functional contribution of β-mannans hydrolysis products.

show abstract

“…in the ileum and cecum. The effect of exogenous enzyme such as β-mannanase and xylanase on intestinal microorganism have been observed in previous studies [36][37][38][39]. Roofchaei et al [40] detected that the dietary supplemental xylanase, β-glucanase, and phytase in combination had an inhibitory effect on the E. coli population in the ileum.…”

Section: Discussionmentioning

confidence: 82%

Synergistic effect of exogenous multi-enzyme and phytase on growth performance, nutrients digestibility, blood metabolites, intestinal microflora and morphology in broilers fed corn-wheat-soybean meal diets

Kim

Ingale²,

Hosseindoust

et al. 2021

Anim Biosci

View full text Add to dashboard Cite

Objective: This study was conducted to investigate the synergistic effect of exogenous multienzyme and phytase on growth performance, nutrients digestibility, blood metabolites, intestinal microflora, and morphology in broilers fed corn-wheat-soybean meal diets. Methods: A 2×2 factorial design was used in this study. Four dietary treatments consisted of i) basal diets (corn-wheat-soybean meal based diets without multi-enzyme and phytase), ii) basal diets with phytase (0.05%), iii) basal diets with exogenous multi-enzyme (0.05%), and iv) basal diets with exogenous multi-enzyme including phytase (0.05%). A total of 480 broiler chickens (Ross 308 - one day old) were weighed and allotted to thirty-two cages (15 birds per cage), and chicks were randomly allocated to four dietary treatments. Results: The body weight gain and feed conversion rate were improved by supplementation of exogenous multi-enzyme containing phytase during the finisher period (p<0.05). The birds fed diets with exogenous multi-enzyme containing phytase had a significantly greater digestibility of dry matter, gross energy, crude protein, calcium, and phosphorus compared with birds fed non-supplemented diets (p<0.05). The chickens fed diets with exogenous multi-enzyme containing phytase showed a higher concentration of Ca and P in the serum (p<0.05). The population of Lactobacillus spp., Escherichia coli, and Clostridium were not affected in the ileum and cecum of chickens fed enzyme-supplemented diets. The dietary supplemental exogenous multi-enzyme containing phytase showed a significant improvement in villus height, crypt depth, and villus height and crypt depth ratio, compared to basal diets or dietary supplemental phytase (p<0.05). Conclusion: The supplementation of the exogenous multi-enzyme containing phytase synergistically improved the growth performance, nutrients digestibility, and villus height of the small intestine of broiler chickens fed a corn-wheat-soybean meal based diets.

show abstract

Effects of β-mannanase on Egg Production Performance, Egg Quality, Intestinal Microbiota, Viscosity, and Ammonia Concentration in Laying Hens

Cited by 6 publications

References 24 publications

Confirmation of Cage Effect and Prebiotic Production Potential of a β-Mannanase, with SBM as Substrate Using Microscopy and Wet Chemistry

Confirmation of Cage Effect and Prebiotic Production Potential of a β-Mannanase, with SBM as Substrate Using Microscopy and Wet Chemistry

Significance of single β-mannanase supplementation on performance and energy utilization in broiler chickens, laying hens, turkeys, sows, and nursery-finish pigs: a meta-analysis and systematic review

Synergistic effect of exogenous multi-enzyme and phytase on growth performance, nutrients digestibility, blood metabolites, intestinal microflora and morphology in broilers fed corn-wheat-soybean meal diets

Contact Info

Product

Resources

About