Grapeseed Meal Used as Natural Antioxidant in High Fatty Acid Diets for Hubbard Broilers

Abstract

The 7-week feeding trial evaluated the effect of 2% grapeseed meal used as natural antioxidant in the diets for slow-growing Hubbard broiler chicks, aged 14 days. The chicks were weighed individually and assigned to two groups (C and E), with 40 chicks per group, housed under conditions of temperature, humidity and light regimen according to the hybrid management guide. The basal ingredients of the conventional diet were corn, wheat, gluten, soybean meal and 2% flaxseed meal, which is rich in polyunsaturated fatty acids. Compared to the control formulation, the experimental formulation included 2% grapeseed meal, both in the growing and finishing stages. The broilers had free access to feed and water. In the end of the feeding trial, blood samples were collected for serum biochemical determinations and six broilers per group were slaughtered and samples of breast and thigh meat were collected. The meat samples were assayed for the basic chemical composition, fatty acids profile and cholesterol content using standardized methods. The content of total polyunsaturated fatty acids (PUFAs) was significantly higher (p<0.05) in breast samples, while cholesterol content was significantly lower (p<0.05) in thigh samples from E group, compared to C. Blood glucose, cholesterol and triglyceride levels were significantly lower (p<0.05) in group E than in group C. The study showed that the grapeseed meal used as natural antioxidant in broiler diets enriched in polyunsaturated fatty acids given to Hubbard broilers had beneficial effects on broiler meat quality and on the metabolic profile of the blood plasma.

Enriching laying hens eggs by feeding diets with different fatty acid composition and antioxidants

“…The fatty acid (FA) profile from samples dried at 65 °C was determined using the fatty acid methyl ester (FAME) gas chromatography according to ISO/TS 17764-2 (2008), as described by Turcu et al 12 . The FA from the total lipid extracts were converted to their methyl esters by trans esterification in methanol containing 3% concentrated sulfuric acid at 80 °C for 4 h. Methyl esters of FA were analysed in a Perkin Elmer-Clarus 500 chromatograph equipped with flame ionization detector (FID) and fitted with a BPX70 capillary column (60 m × 0.25 mm i.d., 0.25 μm film thickness).…”

“…Grape is one of the world's largest fruit crops 6 . Grape seed is a natural agricultural by-product of grapes, considered a natural source of anti-oxidative constituents (vitamin E, flavonoids, pro-anthocyanidins and polyphenols) 12 . Sea buckthorn is widely distributed all over the world.…”

Effect of dietary orange and grapefruit peel on growth performance, health status, meat quality and intestinal microflora of broiler chickens

“…The fatty acids (FA) were determined by gas chromatography method as follows: the fatty acids from the sample are transformed in methyl esters of the fatty acids (FAME) by methylation, followed by component separation in capillary column, identification by comparison with standard chromatograms and quantitative determination of the fatty acids according to the method described by Turcu et al (2019), using Perkin Elmer Clarus 500 gas chromatograph, with capillary column injection system, high polarity stationary phase (BPX70:60m  0.25 mm inner diameter and 0.25 mm thick film).…”

Herbal Plants as Feed Additives in Broiler Chicken Diets

“…Cholesterol concentration of the breast meat samples was determined by gas chromatography, (Perkin Elmer-Clarus 500, GC) according to AOAC International 1996, as described elsewhere (Turcu et al, 2019).…”