Isolation and Probiotic Potential of Lactobacillus Salivarius and Pediococcus Pentosaceus in Specific Pathogen Free Chickens

Abstract

This study was conducted to isolate Lactobacillus salivarius and Pediococcus pentosaceus strains from cecal content and investigate their probiotic potential in specific pathogen free (SPF) chickens. L. salivarius and P. pentosaceus strains were isolated from the cecal content of SPF chickens and identified by 16s rDNA sequence analysis by BLAST analysis at the National Center for Biotechnology Information and phylogenetic analysis using DNAStar software. In an in vivo experiment, 180 7-day-old SPF chickens were randomly assigned into three groups. Group 1 served as a control that was fed a basal diet without probiotic supplementation, and groups 2 and 3 were fed the basal diets supplemented with L. salivarius and P. pentosaceus at 2×108 CFU/g, respectively. Body weight (BW), average daily gain (ADG), feed conversion ratio (FCR), dressing percentage (DP), and the apparent digestibility of crude protein (AD-CP) were calculated. We also determined meat color, fat content, shear force, water content and pH value of breast and thigh muscles; ammonia, urea nitrogen and uric acid content in plasma; fecal ammonia emission level and pH value; and Lactobacillus and Escherichia coli in ceca. Compared with the control group, L. salivarius and P. pentosaceus supplementation significantly increased BW, ADG, DP, AD-CP, fat content of meat, and the number of Lactobacillus in ceca (p<0.05), and decreased FCR, plasma ammonia content, fecal ammonia emission, and pH value and the number of E. coli in ceca (p<0.05). In the in vitro experiment, L. salivarius and P. pentosaceus treatments significantly decreased the ammonia content in medium compared with the control group without probiotic treatment (p<0.05). These results suggest that P. pentosaceus and L. salivarius strains show promising probiotic properties for improving growth, meat quality and microenvironment in chickens and decreasing ammonia content in the medium.

Comparative Genomics of Pediococcus pentosaceus Isolated From Different Niches Reveals Genetic Diversity in Carbohydrate Metabolism and Immune System

“…P. pentosaceus may also exhibit inhibitory activity toward other P. pentosaceus strains differing in antimicrobial production as well as toward P. acidilactici and Lactobacillus plantarum, which may share niches with P. pentosaceus (Luc de Vuyst, 1994). In addition to its antibacterial ability, other functions of P. pentosaceus have also attracted much attention, such as regulating immunity (Silva et al, 2017;Bamba et al, 2018), and improving the growth and final meat quality as well as gut micro-environment of chicken and fish (Dawood et al, 2016;Adel et al, 2017;Chen et al, 2017). Recently, some strains were reported to alleviate toxicity from vital organs such as liver and intestine caused by cadmium (Cd) exposure in a murine model (Dubey et al, 2019).…”

In vitro Assessment of the Probiotic Properties and Bacteriocinogenic Potential of Pediococcus pentosaceus MZF16 Isolated From Artisanal Tunisian Meat “Dried Ossban”

“…According to Hill et al ( 2014 ), probiotics are defined as live microorganisms that when consumed in adequate amounts exert beneficial effects in the host with a GRAS status (Generally Regarded As Safe). Probiotic strains mainly include members of the genera Lactobacillus and Bifidobacterium , but bacteria belonging to the genus Pedioccocus have also been tested and already used (Vidhyasagar and Jeevaratnam, 2012 ; Martino et al, 2013 ; Belhadj et al, 2014 ; Savedboworn et al, 2014 ; Dubey et al, 2015 ; Chen et al, 2017 ). Such investigations have led to an obvious support of the significance of Pediococci in the field of probiotics, indicating that new isolated strains from various food matrices and belonging to this genus may play a key role to be used in new generation of functional foods.…”

Administration of L. salivarius expressing 3D8 scFv as a feed additive improved the growth performance, immune homeostasis, and gut microbiota of chickens

“…The data obtained in this study revealed that the oral administration of L. salivarius expressing 3D8 scFv increased the body weight gain during the overall experimental period. Previous studies, such as those conducted by Chen, Zhu, and Qiu (2017) and Shokryazdan et al (2017), revealed that L. salivarius administered as feed significantly increases the body weight gain of chickens. Balamuralikrishnan, Lee, and Kim (2017) suggested that L. salivarius could increase the body weight gain without affecting the feed intake or feed conversation ratio during the overall experimental period.…”