The Preventive Effects of Different Doses of Glucomannan on Experimental Aflatoxicosis in Japanese Quails

Abstract

This experimental study was performed to investigate whether there is a protective effect of different doses of Glucomannan using against aflatoxicosis in Japanese quail, and pathological changes and relative organ weights were compared. In the experiment, 60 one-day old male Japanese quails were used as divided into six different groups. Experimental groups were designated as Control(C), aflatoxin(A), glucomannan(GM), 2-fold dose of glucomannan(2GM), aflatoxin+glucomannan(A+GM) and aflatoxin+2-fold dose of glucomannan(A+2GM). While control group quails fed the standard ration as ad libitum, other groups were fed with the administrations additionally to standard diet respectively; 2mg/kg of aflatoxin to group A, 1g/kg of glucomannan to group GM, 2g/kg of glucomannan to group 2GM, 2mg/kg of aflatoxin and 1g/kg glucomannan to group A+GM, 2mg/kg of aflatoxin and 2g/ kg glucomannan to group A+2GM. All quails were euthanized at day 21 of the study and organs, (liver, spleen, kidney, thymus and bursa of Fabricius) were removed, weighed and subjected to routine histopathological procedures. Although any important macroscopic changes were not observed in the C, GM and 2GM groups, significant pathological changes were found in the groups of A, A+GM and A+2GM. In the A+GM group, the partial reduction in the severity of microscopic lesions were seen in liver, bursa of Fabricius, thymus and spleen, however a significant reduction in severity of lesions was noticed in A+2GM group. As a result of the study, 2g/kg of glucomannan has been found pathologically to be more effective than 1g/kg glucomannan in terms of the protection against aflatoxicosis by giving orally.

Hepatoprotective and Nephroprotective Effect of Aegle marmelos and Andrographis paniculata in Aflatoxicosis of Broiler Chicken

“…In the present study, no clinical signs and pathological gross lesions could be observed among all the groups, in any of the organs except yellowish discoulouration in liver of T2 group (Fig 1). Similar findings were previously observed among birds exposed to AF by Gholami-Ahangaran (2015), Kumar and Balachandran (2009) and Yavuz et al (2017). Gross appearance of organs such as kidney, spleen, thymus, bursa of Fabricius, gizzard and intestine of all other treatments were identical to that of T1.…”

Impact of Turmeric Curcuma longa on the Body Weight and Liver Function of Japanese Quails Exposed to Dietary Aflatoxins

“…Preparation and Evaluation of Histologic Sections: A part of each liver tissue was fixed in 10% formalin buffer, and was prepared for histological sectioning and staining with hematoxylin and eosin. Histopathological sections were examined under light microscopy (Olympus, Japan) and the lesions were graded according to the established method of Yavuz et al [15].…”

“…Macroscopic and microscopic examinations of the liver are effective methods of studying AF toxicity. Fatty changes are known to be the most common histopathologic lesions caused by AF toxicity in birds' liver [15,18,24]. The fatty changes are probably due to the inhibition or impaired lipid synthesis.…”

“…The proliferation of bile ducts, periportal fibrosis Control: Basal diet; TP: Turmeric powder; AF: Aflatoxins. The order of significance were a<b<c. and leukocytosis are believed to be the regenerative and inflammatory responses of the liver, subsequent to AF toxicity [8,15,24].…”

A Comprehensive Review on the Common Emerging Diseases in Quails.

“…Nigella sativa (black cumin seed) was found to be potent detoxifier for dietary aflatoxins in growing quails as inclusion of these seeds in the diet of quails induced significant improvement in immune responses, meat quality and intestinal E. coli populations (Rasouli-Hiq et al, 2016). Addition of glucomannan (2g/kg of the diet) overcome the adverse effects of aflatoxicosis in 60-day-old Japanese quails and reduced the pathological lesions in liver, kidneys, spleen, thymus glands and bursa of Fabricius (Yavuz et al, 2017). The role of dietary Saccharomyces cerevisiae inclusion to aflatoxin-contaminated diet was studied and the results indicated significant improvements in feed consumption, body weight and feed conversion ratio of Japanese quails (Parlat et al, 2001;Atalay, 2010).…”